Mouse Genetics

项目总结（见说明）： 听力损失是人类最常见的感觉缺陷。在美国，每1000名新生儿中约有7名发生先天性、围产期或早发性听力损失。在出生时患有严重听力障碍的儿童中，约有一半怀疑是遗传因素造成的。在斑马鱼和小鼠中可用的强大的分子和遗传技术结合小鼠和人类听觉之间的功能相似性使这些生物体成为研究耳聋的有用模型系统。 核心D支持遗传模型的用户研究影响听觉、前庭和嗅觉系统功能的基因。我们提供技术支持的形式，专家小鼠饲养（接收，建立杂交，断奶，培养，背景转移等），基因分型，并通知/交付所需的小鼠给用户。Core D还支持几种用于突变体行为和生理分析的设施，包括听觉脑干反应（ABR），简单运动测试，旋转棒测试和前庭眼反射（VOR）分析。其他服务包括获得慢病毒转染技术和用于基因组和蛋白质组数据分析的资源。知识渊博、乐于助人的核心工作人员向所有用户提供咨询和培训。 遗传学核心将为研究人员提供更高的效率，并促进研究人员之间的新合作。季度用户会议提供有关核心资源的信息，讨论协议，并由核心用户讨论他们目前正在进行的研究项目。我们的目标是促进遗传模型的使用，以拓宽我们对通讯障碍的了解，从而改善人类健康。

PROJECT SUMMARY (See instructions): Hearing loss is the most frequent sensory defect in humans. Congenital, perinatal or eariy onset hearing loss occurs in approximately 7 out of 1000 neonates in the United States. In approximately half of the children born with severe hearing impairment, a genetic contribution is suspected. The powerful molecular and genetic techniques available in zebrafish and mouse combined with the functional similarities between mouse and human audition make these organism useful model systems for studying deafness. Core D supports users of genetic models to study genes affecting auditory, vestibular and olfactory system function. We provide technical support in the form of expert mouse husbandry (receiving, setting up crosses, weaning, fostering, background transfers, etc), genotyping, and notification/delivery of requested mice to the users. Core D also supports several facilities for behavioral and physiological analyses of mutants including auditory brainstem responses (ABR), simple motor tests, rotarod testing, and vestibulo-ocular reflex (VOR) analysis. Other services include access to lentiviral transfection technologies and resources for genomic and proteomic data analysis. Advice and training is available to all users from a knowledgeable and helpful Core staff. The Genetics Core will provide greater efficiency for researchers and promote new collaborations between investigators. Quarteriy user meetings provide information on Core resources, discussion of protocols, and talks by Core users on their own research projects currently being pursued. Our goal is to facilitate the use of genetic models to broaden our knowledge of communicafive disorders toward improving human health.