Mouse Genetics

小鼠遗传学

• 批准号: 8526216
• 负责人: JAMES O PHILLIPS
• 金额: $ 13.89万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8526216
• 关键词: Affect; Auditory; Auditory Brainstem Responses; Behavior; Behavioral; Biological Models; Breeding; Caring; Child; Collaborations; Communication; Data Analyses; Defect; Development; Disease; Drosophila genus; Embryonic Development; Equilibrium; Fostering; Genes; Genetic; Genetic Models; Genetic Techniques; Genomics; Genotype; Goals; Health; Hearing; Human; Human Resources; Induced Mutation; Injection of therapeutic agent; Instruction; Knowledge; Lead; Lentivirus Vector; Modification; Molecular Genetics; Motor; Mouse Strains; Mus; National Institute on Deafness and Other Communication Disorders; Notification; Organism; Perinatal; Physiological; Prevention; Process; Production; Proteomics; Protocols documentation; Research; Research Personnel; Research Project Grants; Resources; Role; Rotarod Performance Test; Scientist; Sensory; Services; System; Techniques; Technology; Testing; Training; Transfection; Transgenic Organisms; United States; Weaning; Zebrafish; behavior test; body system; computerized tools; deafness; efficacy research; experience; hearing impairment; improved; meetings; mouse model; mutant; neonate; operation; ranpirnase; vestibulo-ocular reflex

PROJECT SUMMARY (See instructions): Hearing loss is the most frequent sensory defect in humans. Congenital, perinatal or eariy onset hearing loss occurs in approximately 7 out of 1000 neonates in the United States. In approximately half of the children born with severe hearing impairment, a genetic contribution is suspected. The powerful molecular and genetic techniques available in zebrafish and mouse combined with the functional similarities between mouse and human audition make these organism useful model systems for studying deafness. Core D supports users of genetic models to study genes affecting auditory, vestibular and olfactory system function. We provide technical support in the form of expert mouse husbandry (receiving, setting up crosses, weaning, fostering, background transfers, etc), genotyping, and notification/delivery of requested mice to the users. Core D also supports several facilities for behavioral and physiological analyses of mutants including auditory brainstem responses (ABR), simple motor tests, rotarod testing, and vestibulo-ocular reflex (VOR) analysis. Other services include access to lentiviral transfection technologies and resources for genomic and proteomic data analysis. Advice and training is available to all users from a knowledgeable and helpful Core staff. The Genetics Core will provide greater efficiency for researchers and promote new collaborations between investigators. Quarteriy user meetings provide information on Core resources, discussion of protocols, and talks by Core users on their own research projects currently being pursued. Our goal is to facilitate the use of genetic models to broaden our knowledge of communicafive disorders toward improving human health.

项目总结(见说明)： 听力损失是人类最常见的感觉缺陷。在美国，大约每1000名新生儿中就有7名发生先天性、围产期或早发性听力损失。在出生时患有严重听力障碍的儿童中，大约有一半被怀疑是遗传因素造成的。斑马鱼和小鼠拥有强大的分子和遗传技术，再加上小鼠和人类听觉功能的相似性，使这些生物成为研究耳聋的有用模型系统。 核心D支持遗传模型的用户研究影响听觉、前庭和嗅觉系统功能的基因。我们以专家饲养老鼠(接收、设置杂交、断奶、寄养、背景转移等)、基因分型以及向用户通知/交付所需小鼠的形式提供技术支持。Core D还支持多种对突变体进行行为和生理分析的设施，包括听觉脑干反应(ABR)、简单运动测试、转杆测试和前庭眼球反射(VOR)分析。其他服务包括获得慢病毒转基因技术以及基因组和蛋白质组数据分析资源。所有用户都可以从知识渊博、乐于助人的核心员工那里获得建议和培训。 遗传学核心将为研究人员提供更高的效率，并促进研究人员之间的新合作。每季度召开一次用户会议，提供关于核心资源的信息，讨论协议，并由核心用户就他们目前正在进行的研究项目进行演讲。我们的目标是促进遗传模型的使用，以拓宽我们对通讯和五种疾病的知识，从而改善人类健康。