Animal Models to Study Bone Marrow Mesenchymal Stem Cells

研究骨髓间充质干细胞的动物模型

• 批准号: 8337401
• 负责人: PETER MAYE
• 金额: $ 20.69万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-22 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8337401
• 关键词: Adult; Animal Model; Area; Biological Assay; Biology; Bone Marrow; Bone Marrow Cells; Bone Regeneration; Bone Tissue; Calvaria; Cell Fraction; Cell Lineage; Cell surface; Cells; Colony-forming units; Complex; Defect; Development; Environment; Equipment; Fibroblasts; Flow Cytometry; Flushing; Genes; Genetic; Goals; Growth Factor Gene; Histology; Image; Imagery; In Vitro; Knowledge; Maps; Mesenchymal Stem Cells; Mus; Osteoblasts; Photography; Population; Positioning Attribute; Process; Proliferating; Property; Reporter; Reporter Genes; Research; Running; Skeletal Development; Skeletal bone; Specificity; System; Testing; Tetanus Helper Peptide; Therapeutic; Time; Tissues; Transgenes; Transgenic Animals; Transgenic Mice; Transgenic Organisms; Transplantation; Treatment Efficacy; Work; adult stem cell; bone; bone cell; cell type; connective tissue growth factor; experience; immunocytochemistry; in vivo; long bone; magnetic beads; member; multipotent cell; regenerative; repaired; skeletal; skeletal tissue; stem cell population; substantia spongiosa; success; tissue repair

DESCRIPTION (provided by applicant): For many adult skeletal stem cell populations, we lack the ability to identify them in complex tissue environments and determine whether they contribute to the repair of skeletal tissue. The inability to study adult stem cells within their natural environment is a critical barrier that must be overcome in order to advance our understanding of how they contribute to regenerative processes. Here we focus on bone marrow derived mesenchymal stem cells (BMSCs), an adult stem cell population with great therapeutic potential. Genetic approaches in mice can be used that would allow us to identify and fate map BMSCs in bone. Recent developments in our work have identified a new transgenic reporter gene animal model that identifies BMSCs in bone marrow. The objectives of this proposal will continue the characterization of this animal model to confirm our preliminary studies and establish an inducible Cre version that will allow us to fate map this cell population in vivo. Collectively, these animal models will allow us to assay the number of BMSCs present in the bone marrow and determine their contribution over time to skeletal repair.

描述（由申请人提供）：对于许多成人骨骼干细胞群体，我们缺乏在复杂组织环境中识别它们并确定它们是否有助于骨骼组织修复的能力。无法在自然环境中研究成体干细胞是一个必须克服的关键障碍，以促进我们对它们如何促进再生过程的理解。在这里，我们重点研究骨髓间充质干细胞（BMSCs），这是一种具有巨大治疗潜力的成体干细胞群体。小鼠的遗传方法可以让我们在骨骼中识别骨髓间充质干细胞并绘制其命运图。我们最近的工作进展已经确定了一种新的转基因报告基因动物模型，可以识别骨髓中的骨髓间充质干细胞。本提案的目标是继续表征该动物模型，以确认我们的初步研究，并建立一个可诱导的Cre版本，使我们能够在体内绘制该细胞群的命运图。总的来说，这些动物模型将使我们能够测定骨髓中存在的骨髓间充质干细胞的数量，并确定它们对骨骼修复的贡献。