CALERIE Study Biospecimen Repository

描述(由申请人提供)： 动物研究表明，减少卡路里的消耗可以延长寿命，减缓衰老相关疾病的发展。过去对人类卡路里限制的研究有限，但国家老龄化研究所目前正在支持一项为期两年的健康年轻男女研究，其中一些人将有正常的饮食，另一些人将减少25%的卡路里摄入量。将收集血液、尿液和组织样本，以便研究人员寻找两组之间可能出现的差异。更具体地说，研究人员将确定在长寿、健康的人身上观察到的变化是否在摄入较少卡路里的志愿者身上发现。 研究计划要求收集更多的样本，该项目将提供一个冷冻库和液氮储存库，将额外的样本储存在其中以供将来测试。这个储存库将是一个有价值的研究资源，因为冷冻的样品将可用于测试关于老化的新想法，并用于使用将在研究期间和之后开发的新测试技术。以前的研究表明，对冷冻样本进行的测试是有效的，即使在最初收集样本15年或更长时间后，与各种类型研究相关的冷冻样本储存库也被证明对研究人员有用。该项目将支持储存库的维护和运作，包括温度监测警报、冰柜的后备电源、储存库的安全和记录保存的费用。此外，该项目将支持储存库工作人员的工资，他们将与研究人员合作，确定这些冷冻样本是否对他们的研究有用，以及是否有适当的样本可用。当研究人员的样本请求获得批准时，储存库人员将仔细确定要从储存库中移除哪些样本，将其适当包装，运往研究人员的实验室，并更新储存库记录。 拥有这项研究的样本库将有助于研究人员确定减少卡路里是否会导致在健康老年人中观察到的身体化学变化。如果这是大体上正确的，那么人们会考虑减少卡路里的摄入量，以此来增加他们活得更长、随着年龄的增长保持健康的机会。

DESCRIPTION (provided by applicant): Animal studies have shown that reducing the number of calories consumed extends the lifespan and slows the development of aging-related diseases. Studies of caloric restriction in humans have been limited in the past, but the National Institute on Aging is currently supporting a two- year study of healthy young men and women in which some will have a normal diet and others will consume 25% fewer calories. Samples of blood, urine, and tissue will be collected so that researchers can look for differences that may develop between the two groups. More specifically, researchers will be determining whether changes that have been observed in people living long, healthy lives are found in the volunteers who consume fewer calories. The study plan calls for extra samples to be collected, and this project would provide for a repository of freezers and liquid nitrogen storage units in which the extra samples would be stored for future testing. This repository will be a valuable research resource, as the frozen samples will be available for testing new ideas about aging and for using new testing techniques that will be developed during and after the study. Previous studies have shown that testing performed on frozen samples is valid, and repositories of frozen samples associated with studies of various types have proven useful to researchers even 15 or more years after the samples were originally collected. This project would support the maintenance and operation of the repository, covering the costs of temperature monitoring alarms, back-up power for the freezers, security for the repository, and record-keeping. Additionally, this project will support the salaries of repository staff members who will work with researchers to determine whether these frozen samples would be useful in their research and whether appropriate samples are available. When a researcher's request for samples is approved, repository personnel will carefully determine which samples to remove from the repository, pack them appropriately, ship them to the researcher's laboratory, and update the repository records. Having a repository of samples from this study will assist researchers as they try to determine whether cutting calories leads to changes in body chemistry that have been observed in healthy older people. If this is generally true, then people would want consider reducing their calorie intake as a way to increase their chances of living longer and being healthy as they age.