Development of a Biomarker for Dietary Furanocoumarins

膳食呋喃香豆素生物标志物的开发

• 批准号: 8305071
• 负责人: Sabrina Trudo
• 金额: $ 14.9万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-24 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8305071
• 关键词: Address; Animal Model; Apiaceae; Area; Biological; Biological Assay; Biological Markers; Botanicals; CYP1A2 gene; CYP3A4 gene; Carcinogen Metabolism; Carcinogens; Carrots - dietary; Cause of Death; Characteristics; Chemopreventive Agent; Chemoprotective Agent; Chinese People; Citrus; Collaborations; Complement; Cytochrome P-450 CYP1A1; DNA Adducts; Data; Development; Diet; Diet Research; Dietary Practices; Dose; Drug Metabolic Detoxication; Eating; Edible Plants; Enzymes; Epidemiologic Studies; Epidemiology; Equilibrium; Excretory function; Exposure to; Faculty; Family; Food; Frequencies; Fruit; Furanocoumarins; Goals; Health; Hour; Human; In Vitro; Individual; Instruction; Intake; Intervention; Investigation; Isothiocyanates; Knowledge; Malignant Neoplasms; Measurement; Mediating; Metabolic; Metabolic Biotransformation; Methodology; Methods; Minnesota; Pattern; Phase; Phytochemical; Prevention Research; Principal Investigator; Questionnaires; Records; Reporting; Research; Research Activity; Resources; Rutaceae; Sampling; Second Primary Cancers; Singapore; Source; Spottings; Testing; Time; United States; Urine; Vegetables; Xenobiotics; anticancer research; base; bioactive food component; cancer prevention; cancer risk; carcinogenesis; career; cohort; cytochrome P-450 CYP2A6 (human); diet and cancer; feeding; fruits and vegetables; human data; interest; prospective; response; skills; tool; urinary

This project expects to develop the career of the candidate through didacticand research activities that complement the candidate's 5-year goal of studyingfuranocoumarins and cancer prevention in humansand 10- year goal ofstudying diet patterns and protection against cancer. Specifically, the career developmentplan includes the following objectives: 1) acquire knowledge and skillsin biomarker development,2) develop skills in epidemiologic study of diet and cancer prevention, and 3) build interdisciplinaryresearch associations and collaborations. The research plan will utilize the extensive resources and expertise at the Universityof Minnesota Masonic CancerCenter, an NCIdesignated ComprehensiveCancerCenter,to address two specific aims. The first aim is to develop a urinary biomarker of dietary exposure to furanocoumarins which are a group of compoundsthat may decrease cancer risk through inhibiting.activation of carcinogens. The second aim is to validate the biomarker for use in epidemiologicstudies to generate more human data in this area ofcancer prevention research. Senior faculty from the MasonicCancer Center will provide scientific and professional guidance in addition to the data and urine samples from a large prospective cohort in Singapore. First,we will modify existing methods for furanocoumarin analysis ofplants and various human biospecimens and develop a method for use with pooled human urine samples spiked with the analytes of interest. After determinationof the necessary conditionsand steps for optimal characterization of each analyte, we will chemically validate the method usingurine samples from 10 individualsgiven controlled amounts of furanocoumarin food sources and study excretion time and dose response. Lastly,usingdiet intake data (obtained by food frequeny questionnaire) and urine samples from the Singapore Chinese HealthStudy, we will validate the use of the urinary furanocoumarin biomarker with spot urine samples. This will includeanalysis of furanocoumarin food sources from Singaporeto calculate furanocoumarin intake based on the food frequencyquestionnaire data. RELEVANCE (See instructions): The results of this project will provide a much-needed tool for subsequent study of whether dietary furancoumarins can decrease the risk of cancer, the second leading cause of death in the United States. The project will also expand the number of dietary biomarkers available and the ability to assess whether various diet patterns protect against cancer.

该项目预计将通过教学和研究活动， 补充候选人的研究呋喃香豆素和人类癌症预防的5年目标和10- 年的目标是研究饮食模式和预防癌症。具体而言，职业发展计划 包括以下目标：1）获得生物标志物开发的知识和技能，2）开发 饮食与癌症预防的流行病学研究，3）建立跨学科研究协会， 合作。该研究计划将利用大学的广泛资源和专业知识。 明尼苏达共济会癌症中心，一个NCIdesignated综合癌症中心，以解决两个具体的 目标。第一个目标是开发一种饮食暴露于呋喃香豆素的尿液生物标志物， 通过抑制致癌物的活化来降低癌症风险的化合物。第二个目标是 验证用于流行病学研究的生物标志物，以产生更多癌症领域的人类数据 预防研究。来自共济会癌症中心的高级教师将提供科学和专业的 除了来自新加坡一个大型前瞻性队列的数据和尿样外，一是 修改现有的植物和各种人类生物标本的呋喃香豆素分析方法， 用于加标目标分析物的合并人尿样的方法。在确定 每种分析物的最佳表征的必要条件和步骤，我们将化学验证 方法使用尿液样本从10个人给予控制量的呋喃香豆素食物来源， 研究排泄时间和剂量反应。最后，使用饮食摄入数据（通过食物频率获得）， 问卷）和尿液样本从新加坡华人健康研究，我们将验证使用的 尿呋喃香豆素生物标志物与点尿样品。这将包括对呋喃香豆素食品的分析 根据食物频率问卷数据计算呋喃香豆素摄入量。 相关性（参见说明）： 该项目的结果将为随后的研究提供急需的工具， 在美国，糠醛可以降低癌症的风险，而癌症是导致死亡的第二大原因。 该项目还将扩大可用的饮食生物标志物的数量，以及评估是否 不同的饮食模式可以预防癌症。