Development of a Biomarker for Dietary Furanocoumarins

该项目希望通过教学和研究活动来发展候选人的职业生涯， 补充候选人研究呋喃香豆素和人类癌症预防的5年目标和10- 研究饮食模式和预防癌症的年度目标。具体来说，职业发展计划 包括以下目标：1)获得生物标记物开发方面的知识和技能；2)发展 饮食和癌症预防的流行病学研究，以及3)建立跨学科研究协会和 合作。该研究计划将利用加州大学的广泛资源和专业知识。 明尼苏达州共济会癌症中心，NCI指定的综合性癌症中心，以解决两个具体的问题 目标。第一个目标是开发一种饮食暴露于呋喃香豆素的尿液生物标记物，这是一组 可以通过抑制致癌物质的激活来降低癌症风险的化合物。第二个目标是 验证生物标记物在流行病学研究中的使用，以在该癌症领域产生更多的人类数据 预防研究。来自共济会癌症中心的资深教员将提供科学和专业的 除了来自新加坡一个大型预期队列的数据和尿样外，还提供了指导。首先，我们将 修改现有的植物和各种人类生物制剂中呋喃香豆素的分析方法，并开发一种 与添加了感兴趣的分析物的人尿样混合使用的方法。在确定之后 每个分析物的最佳表征所需的条件和步骤，我们将在化学上验证 方法使用10个人的尿样，给出受控数量的呋喃香豆素食物来源和 研究排泄时间和剂量反应。最后，使用饮食摄入量数据(通过食物频率获得 问卷)和来自新加坡华人健康研究中心的尿样，我们将验证 尿呋喃香豆素生物标志物与现场尿样。这将包括对呋喃香豆素食品的分析 来自新加坡的消息来源根据食物频率问卷数据计算呋喃香豆素的摄入量。 相关性(请参阅说明)： 这一项目的结果将为后续的饮食研究提供急需的工具 呋喃香豆素可以降低癌症的风险，癌症是美国第二大死亡原因。 该项目还将扩大可用的饮食生物标记物的数量，以及评估 不同的饮食模式可以预防癌症。

This project expects to develop the career of the candidate through didacticand research activities that complement the candidate's 5-year goal of studyingfuranocoumarins and cancer prevention in humansand 10- year goal ofstudying diet patterns and protection against cancer. Specifically, the career developmentplan includes the following objectives: 1) acquire knowledge and skillsin biomarker development,2) develop skills in epidemiologic study of diet and cancer prevention, and 3) build interdisciplinaryresearch associations and collaborations. The research plan will utilize the extensive resources and expertise at the Universityof Minnesota Masonic CancerCenter, an NCIdesignated ComprehensiveCancerCenter,to address two specific aims. The first aim is to develop a urinary biomarker of dietary exposure to furanocoumarins which are a group of compoundsthat may decrease cancer risk through inhibiting.activation of carcinogens. The second aim is to validate the biomarker for use in epidemiologicstudies to generate more human data in this area ofcancer prevention research. Senior faculty from the MasonicCancer Center will provide scientific and professional guidance in addition to the data and urine samples from a large prospective cohort in Singapore. First,we will modify existing methods for furanocoumarin analysis ofplants and various human biospecimens and develop a method for use with pooled human urine samples spiked with the analytes of interest. After determinationof the necessary conditionsand steps for optimal characterization of each analyte, we will chemically validate the method usingurine samples from 10 individualsgiven controlled amounts of furanocoumarin food sources and study excretion time and dose response. Lastly,usingdiet intake data (obtained by food frequeny questionnaire) and urine samples from the Singapore Chinese HealthStudy, we will validate the use of the urinary furanocoumarin biomarker with spot urine samples. This will includeanalysis of furanocoumarin food sources from Singaporeto calculate furanocoumarin intake based on the food frequencyquestionnaire data. RELEVANCE (See instructions): The results of this project will provide a much-needed tool for subsequent study of whether dietary furancoumarins can decrease the risk of cancer, the second leading cause of death in the United States. The project will also expand the number of dietary biomarkers available and the ability to assess whether various diet patterns protect against cancer.