Cell Biology and Multigene Transcriptional Profiling Core
细胞生物学和多基因转录分析核心
基本信息
- 批准号:8378445
- 负责人:
- 金额:$ 18.55万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2002
- 资助国家:美国
- 起止时间:2002-06-13 至
- 项目状态:未结题
- 来源:
- 关键词:AntibodiesApoptosisBiological PreservationBreast Cancer CellCancer cell lineCell Culture TechniquesCell CycleCell LineCellsCellular biologyCharacteristicsColon CarcinomaComplementary DNAConsultCultured CellsDataData SetDermalEndothelial CellsEnsureGene ExpressionGene Expression ProfilingGenesGrowthGrowth FactorHeartHumanIndividualLeadLewis Lung CarcinomaLibrariesLungMaintenanceMalignant Epithelial CellMeasurementMeasuresMessenger RNAMolecular ProfilingMonitorMusNamesPhenotypePlayProcessQuality ControlRattusReagentRegulationResearch PersonnelSamplingSerumStatistical Data InterpretationTechniquesTestingTimeUmbilical veinValidationangiogenesisbasedesigndimerfetalglioma cell linemeetingsnovel markerprogramsrepositoryspellingtissue culture
项目摘要
The Cell Biology and Gene Profiling Core will provide standardized, homogenous and well-characterized cell
cultures of human and mouse origin to the investigators of the individual projects. In addition, this Core will
provide multi-gene transcriptional profiling (MGTP) for the characterization of cells in culture and tissues.
The core will produce human dermal endothelial cells (HDMEC), mouse heart and lung endothelial cells,
ymphatic endothelial cells, and large-scale amplification of human umbilical vein endothelial cells (HUVEC).
The core has developed a panel of antibodies for endothelial cell markersthat will be used to monitor
preservation of phenotype in culture and will function as a repository for cell lines that are generated by the
orogram. The Cell Biology and Gene Profiling Core will also be responsible for quality control testing of
certain key reagents (e.g. fetal serum, growth factors, media) and the bulk purchase of these reagents for
the use of all projects. This function ensures consistent growth and maintenance conditions for cultured cells
from their isolation and propagation within the core through their manipulation in the various individual
projects. This core will also culture the various cancer cell lines that will be used within the project.
Through MGTP (multi-gene transcriptional profiling), this core also makes available to the investigators of the
individual projects a facility for accurate quantification of gene expression. MGTP is a gene expression
profiling technique based on quantitative real-time PCR using primers designed for uniform amplification
efficiencies and a master cDNA template for high experimental throughput for multi-gene panels. MGTP is
capable of accurately and reproducibly measuring mRNA copy numbers expressed per cell. Our primer
library currently includes over 1000 different human and mouse genes that play critical functions in
angiogenesis, the cell cycle, and apoptosis, and we will continue to grow the library by several hundred
genes per year. The Core will provide expertise in the statistical analysis of the gene profiling data. MGTP
will also be used to characterize the HDMEC that are prepared by the core to assure lot-to-lot consistency
and to develop new markers.
细胞生物学和基因分析核心将提供标准化,同质和良好表征的细胞
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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John W LAWLER的其他文献
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{{ truncateString('John W LAWLER', 18)}}的其他基金
Inhibition of angiogenesis by thrombospondin-1 (A2)
血小板反应蛋白-1 (A2) 抑制血管生成
- 批准号:
8193192 - 财政年份:2009
- 资助金额:
$ 18.55万 - 项目类别:
Inhibition of angiogenesis by thrombospondin-1 (A2)
血小板反应蛋白-1 (A2) 抑制血管生成
- 批准号:
7876959 - 财政年份:2009
- 资助金额:
$ 18.55万 - 项目类别:
Inhibition of angiogenesis by thrombospondin-1 (A2)
血小板反应蛋白-1 (A2) 抑制血管生成
- 批准号:
8270525 - 财政年份:2009
- 资助金额:
$ 18.55万 - 项目类别:
Inhibition of angiogenesis by thrombospondin-1 (A2)
血小板反应蛋白-1 (A2) 抑制血管生成
- 批准号:
7650776 - 财政年份:2009
- 资助金额:
$ 18.55万 - 项目类别:
Thrombospondins and other Matricellular Proteins in Tissue Organization and Hemeo
组织组织和血红蛋白中的血小板反应蛋白和其他基质细胞蛋白
- 批准号:
7275863 - 财政年份:2007
- 资助金额:
$ 18.55万 - 项目类别:
Cell Biology and Multigene Transcriptional Profiling Core
细胞生物学和多基因转录分析核心
- 批准号:
8459039 - 财政年份:2002
- 资助金额:
$ 18.55万 - 项目类别:
Structure and Function of Thrombospondin-1
Thrombospondin-1 的结构和功能
- 批准号:
6625601 - 财政年份:2002
- 资助金额:
$ 18.55万 - 项目类别:
Structure and Function of Thrombospondin-1
Thrombospondin-1 的结构和功能
- 批准号:
6477678 - 财政年份:2002
- 资助金额:
$ 18.55万 - 项目类别:
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