Cell Biology and Cellular Imaging

总体目标和依据： 现有的神经科学和成像核心已经重组，现在被命名为“细胞生物学和细胞成像”。“我们的目标是提供专业知识，服务和设备，以广泛支持细胞和分子神经科学研究，在我们的中心，重点是阐明与影响CNS发育和功能的遗传和环境诱导的发育疾病相关的病理生理学机制。我们使用最先进的仪器，如蔡司激光扫描共聚焦显微镜LSM 510 Meta和徕卡激光细胞捕获显微切割系统，升级了组织学和细胞成像的组织处理。我们还配备了3个新的细胞培养室，以支持我们对神经干/祖细胞培养方法的不断扩大的需求，以研究神经胶质和神经元细胞生物学， 移植研究。组织学部分现已取消，因为BRI组织学核心现已提供类似服务。虽然提出了一个新的和单独的干细胞核心集中在人类胚胎干细胞（核心C），细胞生物学和细胞成像核心将继续支持啮齿动物和人脑细胞亚群的细胞培养，并将扩大细胞成像组件。对后者的需求急剧增长，尤其是在研究发育性疾病动物模型的教师中大量使用。我们还将启动一个新的子组件，以提供细胞免疫学领域的专业知识。这是随着越来越多的人认识到免疫功能和/或炎症反应影响大量IDD而完成的，并且是人类研究以及我们许多动物模型中研究的关键组成部分。这一新功能包含在本核心中，因为它利用细胞培养， 细胞成像作为主要工具，尽管在许多情况下方法是高度专业化的，例如，使用流式细胞术检查和研究免疫应答的细胞和分子特征。

Overall Objective and Rationale: The existing Neuroscience and Imaging Core has been reorganized and is now entitled "Cell Biology and Cellular Imaging." The aim is to provide expertise, services and equipment to broadly support cellular and molecular neuroscience research which, in our Center, is focused on elucidating the mechanisms of pathophysiology associated with the genetic and environmentally-induced developmental diseases affecting CNS development and function. We upgraded tissue processing for histology and cellular imaging with stateof-the art instruments like the Zeiss Laser Scanning Confocal Microscope LSM 510 META and the Leica Laser Cell Capture Microdissection system. We also equipped 3 new cell culture rooms to support our expanding needs for neural stem/progenitor cell culture methodologies to study glial and neuronal cell biology and for transplantation studies. The histology component has now been eliminated, as similar services are now available from the BRI histology core. Although a new and separate Stem Cell Core is proposed to focus on human embryonic stem cells (Core C), the Cell Biology and Cellular Imaging Core will continue to support cell culture of rodent and human brain cell subpopulations, and will expand the cellular imaging component. The demand for the latter has grown dramatically, becoming particular heavily used by faculty working with animal models of developmental diseases. We will also initiate a new subcomponent to provide expertise in the area of cellular immunology. This is done with the growing recognition that immune function and/or inflammatory responses impact a great number of IDD, and is a critical of component of investigation in human studies as well as our many animal models. This new function is included in this Core because it utilizes cell culture and cellular imaging as principle tools, although the methodologies in many cases are highly specialized, for example, the use of FACS to examine and investigate the cellular and molecular character of the immune response.