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Hydrodynamic Chirality: A novel method for chiral separation and analysis

流体动力学手性：一种手性分离和分析的新方法

基本信息

批准号：
8445275
负责人：
Osman Kibar
金额：
$ 56.56万
依托单位：
DYNAMIC CONNECTIONS, LLC
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-01-01 至 2014-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8445275
关键词：
Adverse effects Agriculture Analytical Chemistry Antibodies Binding Blood capillaries Cells Chemicals Chemistry Chromatography Complement Computer software Coupling Crystallization Crystallography Custom Dependence Detection Electronics Feedback Frequencies Hand Handedness High Pressure Liquid Chromatography Industry Institution Lead Left Libraries Marketing Methods Microfluidics Miniaturization Modeling Molecular Motion Performance Pharmaceutical Chemistry Pharmaceutical Preparations Pharmacologic Substance Phase Physics Preparation Process Recording of previous events Research Research Personnel Risk Rotation Running Sampling Savings Services Small Business Innovation Research Grant Solutions Solvents System Techniques Technology Testing Therapeutic Index Time Universities Vibrational Circular Dichroism Work capillary catalyst chiral molecule commercial application commercialization cost design drug development drug discovery electric field enantiomer flexibility improved innovation instrument milligram molecular dynamics molecular mechanics nanoscale novel polarized light prototype public health relevance real world application screening small molecule stereochemistry tool voltage

项目摘要

DESCRIPTION (provided by applicant): In this SBIR Fast Track we focus on a novel chiral chemistry process technology that enables baseline separation, identification of absolute stereochemistry and purity analysis, all in one instrument and within minutes. The technology offers significant cost and time savings in chiral chemistry processing through inexpensive components and predictive software. Briefly, when placed in a capillary and exposed to a Rotating Electric Field (REF), left and right-handed chiral molecules rotate with the field and act as nanoscale propellers with respect to their solvent. Due to their opposite handedness, they propel along the axis of field rotation in opposite directions, enabling both isolation and analysis, including absolute configuration determination. Current techniques for chiral analysis and isolation such as high pressure liquid chromatography, x-ray crystallography and vibrational circular dichromism are frequently time consuming, expensive, low fidelity and are generally hindrances to the widespread study of chiral chemistry. This proposed new technique, relying on hydrodynamic chirality and principles of physics, promises to enable the creation of an easy-to-use, order of magnitude less expensive, benchtop instrument for chiral analysis and isolation. With more researchers, companies and universities able to afford chiral analysis and isolation, the pace of chiral drug study, discovery and commercialization is bound to increase. The FDA, due to the generally proven benefits of single enanatiomer compounds, mandates that most new drugs be enantiospecific, increasing the need for new chiral separation and analysis techniques. In Phase I, the Specific Aims are to 1) Design and build a robust and reliable REF separation chamber operating at up to 300MHz and 100V p-p; 2) Demonstrate quantitatively, 99% pure enantiomeric separation and high fidelity absolute configuration determination of at least two chiral molecules in both polar and nonpolar solvents; and 3) Investigate design parameter tradeoffs including voltage and frequency dependence and throughput up to gram per day scale. We will use rotating electric fields, microfluidics and chiral detection to achieve chiral analysis and isolation. Once the technique is proven in Phase I, for Phase II the Specific Aims 1) Design & build a board- level-integrated separation assembly; 2) Design a disposable separation chamber with cost of goods less than $10 at analytical scale; 3) Develop and integrate predictive software with high fidelity and ease-of-use for non-experts; 4) Demonstrate broad applicability for at least 100 chiral molecules in polar and nonpolar solvents; and 5) Demonstrate baseline separation at milligram scale. In addition to Phase I methods, we will use ab initio, molecular mechanics and molecular dynamics simulations to determine propulsion direction and velocity.

描述（由申请人提供）：在SBIR快速通道中，我们专注于一种新型手性化学工艺技术，该技术可在一台仪器中在几分钟内实现基线分离、绝对立体化学鉴定和纯度分析。该技术通过廉价的组件和预测软件在手性化学加工中节省了大量成本和时间。简而言之，当置于毛细管中并暴露于旋转电场（REF）时，左手和右手手性分子随场旋转并相对于其溶剂充当纳米级推进器。由于其相反的旋向性，它们沿相反方向沿着场旋转轴推进，从而实现隔离和分析，包括绝对配置确定。目前用于手性分析和分离的技术，如高压液相色谱、X射线晶体学和振动圆二色性通常是耗时、昂贵、低保真度的，并且通常是手性化学的广泛研究的障碍。这种拟议的新技术，依赖于流体动力学手性和物理原理，有望使创建一个易于使用，数量级更便宜，台式仪器手性分析和隔离。随着越来越多的研究人员、公司和大学能够负担得起手性分析和分离，手性药物研究、发现和商业化的步伐必将加快。由于单一对映体化合物的普遍证明的益处，FDA要求大多数新药是对映体特异性的，增加了对新的手性分离和分析技术的需求。在第一阶段，具体目标是：1）设计和建立一个稳定可靠的REF分离室，工作频率高达300 MHz和100 V p-p; 2）定量演示，99%纯对映体分离和高保真绝对构型确定至少两个手性分子在极性和非极性溶剂中;以及3）研究设计参数折衷，包括电压和频率依赖性以及高达克/天规模的吞吐量。我们将使用旋转电场，微流体和手性检测来实现手性分析和分离。一旦该技术在第一阶段得到证明，对于第二阶段，具体目标是1）设计和构建板级集成的分离组件; 2）设计在分析规模下商品成本低于10美元的一次性分离室; 3）开发和集成对于非专家具有高保真度和易用性的预测软件; 4）证明在极性和非极性溶剂中对至少100个手性分子的广泛适用性;和5）证明在毫克规模下的基线分离。除了第一阶段的方法，我们将使用从头算，分子力学和分子动力学模拟，以确定推进方向和速度。