Mechanism Underlying Nitrite Sensitivity of Mucoid Pseudomonas in COPD

COPD 中粘液假单胞菌亚硝酸盐敏感性的机制

• 批准号: 8391607
• 负责人: DANIEL J. HASSETT
• 金额: --
• 依托单位: CINCINNATI VA MEDICAL CENTER RESEARCH
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-10-01 至 2013-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8391607
• 关键词: Accounting; Adverse effects; Aerobic; Affect; Alginates; Anabolism; Animal Model; Animals; Bacteria; Bathing; Biochemistry; Biological Models; Blood; Caring; Cell Death; Cells; Cerebrospinal Fluid; Chronic; Chronic Bronchitis; Chronic Obstructive Airway Disease; Clinical Trials; Communities; Complex; Diagnosis; Dyspnea; Expenditure; Exposure to; Genes; Genetic; Genetic Transcription; Goals; Growth; Health Care Costs; Hospitalization; Hospitals; Human; In Vitro; Infection; Journals; Kinetics; Libraries; Liquid substance; Lung; Lung diseases; Mediating; Microbial Biofilms; Modeling; Molecular; Morbidity - disease rate; Mucous body substance; Mus; Mutation; NBL1 gene; Nitric Oxide; Nitrites; Opportunistic Infections; Organism; Paper; Patients; Pattern; Play; Prevalence; Protein S; Proteins; Pseudomonas; Pseudomonas aeruginosa; Publishing; Pulmonary Emphysema; Relative (related person); Resistance; Role; Sigma Factor; Site; Sodium Nitrite; System; Testing; Thick; Urinary tract; airway epithelium; airway surface liquid; base; bronchial epithelium; cystic fibrosis airway; design; improved; in vivo; killings; member; mortality; mouse model; mucoid; mutant; novel; public health relevance; research study; respiratory

DESCRIPTION (provided by applicant): Nearly 40% of Cincinnati VA patients suffer from chronic obstructive pulmonary disease (COPD) that often suffer from airway infection by opportunistic bacteria, the most prevalent of which is Pseudomonas aeruginosa (PA). PA is found at high titers in chronically infected COPD airways and many strains are mucoid, resulting from overproduction of a viscous exopolysaccharide called alginate. The major mechanism of mucoid conversion of PA is via mutations. These mutations occur predominantly (>84-92%) within mucA, encoding an anti-sigma factor. Without MucA, the sigma factor AlgT(U) directs transcription of genes involved in alginate biosynthesis, resulting in mucoidy. In 2006, we published a paper in the Journal of Clinical Investigation, demonstrating that mucoid mucA mutant bacteria are killed during anaerobic exposure to acidified nitrite (A- NO2-). However, inactivation of algT(U) in the mucA background did not relieve sensitivity to acicified nitrite strongly indicating the affects observed were MucA-specific. Provision of mucA in trans restored A-NO2- resistance and subsequent experiments established that nitric oxide (NO) plays a role in cell death. Importantly, no adverse effects were observed when A-NO2- was applied to human airway epithelia. In summary, we have discovered a novel, non-toxic agent that could potentially achieve the translational goal of eradicating mucoid PA from the airways of COPD patients. Three specific aims are proposed and designed to determine (i) the mechanism(s) underlying A-NO2- sensitivity in mucA mutant bacteria, (ii) the role of MucA and members of the anaerobic respiratory cascade in biofilm sensitivity to A-NO2-, and (iii) to test the hypothesis that mucA and double anaerobic regulator mutants will be even more sensitive to A-NO2- in a tried-and-true mouse model of chronic lung infection. Aim 1. Identify the molecular basis underlying anaerobic acidified NO2- sensitivity in mucoid mucA mutant PA. Although our discovery in 2006 describes an "Achilles' Heel" of mucoid, mucA mutant bacteria, we still do not know the mechanism of killing of these organisms by A-NO2-. Specifically, the role of MucA, NO3-/NO2- transport, anaerobic regulatory machinery and NO-sensitive sulfhydryl/Fe-containing proteins is very much underappreciated. The molecular basis will be determined by (i) micoarray studies of mucA and wild-type strains grown under aerobic and anaerobic conditions; (ii) determination of the cellular MucA levels that allow nitrite sensitivity, (iii) determining the rates/levels of NO2- and NO3- transport in mucA and WT and mucA double (anaerobic regulatory hierarchy genes) and; (iv) elucidate the status of critical cellular proteins known to be targets of nitrosylation. Aim 2. Determine the effects of NO2- on viability of wild-type versus mucoid, DmucA mutants in complex, highly organized communities known as biofilms using 3 different established model systems. The biofilm mode of growth is that which has been determined to exist and actually thrive within the thick CF airway mucus. We will use 3 complimentary yet contrasting approaches that include (i) a static biofilm system, representing the stagnant mucus of COPD airways, (ii) a flow-through system that represents a contrasting biofilm mode of growth, and finally (iii) growth is static biofilms in airway surface liquid derived from human primary cells. Aim 3. Determine the effects of NO2- on viability of wild-type versus DmucA and double mucA anaerobic regulatory mutants in an established murine chronic lung infection model. Proof-of-principle animal studies are required to show the relative efficacy of the aforementioned treatments on not only mucA mutant organisms, but also mucA mutants with selected second site mutations in genes encoding proteins that are S-nitrosylated upon exposure to A-NO2-.

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