权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Title: Dielectric properties of chondrocytes in a changing environment.

标题：变化环境中软骨细胞的介电特性。

基本信息

批准号：
8690767
负责人：
Michael William Stacey
金额：
$ 15.97万
依托单位：
OLD DOMINION UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-07-01 至 2016-02-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8690767
关键词：
Address Animals Arrhythmia Attention Binding Biology Biomechanics Calcium Signaling Cartilage Cell Count Cell Size Cell membrane Cell physiology Cells Cellular biology Charge Chondrocytes Chronic Cystic Fibrosis Cytoplasm Cytoskeleton Defect Deposition Devices Disease Drug Targeting Dysplasia Electric Capacitance Electrolytes Electrophysiology (science)Environment Epilepsy Equilibrium Event Fostering Gene Expression Health Heterogeneity Homeostasis Housing Human Hypoxia Intervention Investigation Ion Channel Ions Measurement Measures Mechanics Membrane Microfluidic Microchips Microfluidics Miniaturization Movement Muscle Myotonia Nerve Osmolalities Osmolar Concentration Pathway interactions Pharmaceutical Preparations Physiological Play Production Proteins RNA Regulation Research Reverse Transcriptase Polymerase Chain Reaction Role Spectrum Analysis Stimulus System Targeted Toxins Therapeutic Therapeutic Intervention Time Tissues Water cell type dielectric property electrical potential experience extracellular novel pressure public health relevance response

项目摘要

DESCRIPTION (provided by applicant): Chondrocytes, the only cell type that produces cartilage, occupy a unique bioelectrical environment. Mechanical load generates bioelectrical stimulation through rapid changes in ionic and water content around cells. A chondrocytes response to changing ionic gradients plays an essential role in regulation of extra cellular matrix synthesis. An abnormal response by the cell may result in abnormal deposition of proteins leading to degeneration of tissue with pathological consequences. Typically, response to ionic and osmotic gradients across cell membranes is by ion movement through channels as a cell reacts to maintain equilibrium. Chondrocytes possess an array of ion channels on par with electrically excitable tissues, yet chondrocyte electrophysiology is much understudied. Ion channel presence in chondrocytes is now becoming apparent, however, extensive studies in human chondrocytes and how potential channel defects relate to disease are understudied. This proposal will quantify in real time dielectric properties of the membrane and cytoplasm in response to electrochemical changes to cells related to ion channel gene expression. We hypothesize that dielectric response of chondrocytes to changes in environment will be dependent upon ion channel activity. The objectives of this proposal are (1) to document dielectric changes in chondrocytes in real time when exposed to changes in osmolality, pH and ion flow using a novel microfluidic device. (2) Measure gene expression of ion channels in chondrocytes as a response to these changes. (3) Chondrocytes function under hypoxic conditions. We will directly compare dielectric response under normal versus hypoxic conditions and identify ion channels active in chondrocytes under hypoxic conditions. To our knowledge, our results will be the first description of dielectric properties of chondrocytes measured in real time and correlated to ion channel expression under hypoxia and will fundamentally impact cartilage biology unifying biomechanical and bioelectrical events in cartilage through ion channel response.

描述（由申请人提供）：软骨细胞是唯一产生软骨的细胞类型，占据独特的生物电环境。机械负荷通过细胞周围离子和水含量的快速变化产生生物电刺激。软骨细胞对离子梯度变化的响应在细胞外基质的调节中起着重要作用