Core E

核心E

• 批准号: 8879400
• 负责人: GARNETT H KELSOE
• 金额: $ 19.16万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8879400
• 关键词: Affinity; Antibody Affinity; Antigen-Antibody Complex; Antigens; B cell repertoire; B-Lymphocytes; Biotechnology; Case Study; Cell Culture System; Cell Lineage; Cells; Data; Development; Epitopes; Generations; Hemagglutinin; Human; Immunity; Immunization; Infection; Influenza; Joints; Maps; Modeling; Mus; Mutation; Names; Nature; Population; Reaction; Resolution; Seasons; Structure; Structure of germinal center of lymph node; Surface; Testing; Vaccines; Variant; Virus; Work; base; design; flu; immunogenic; influenza virus vaccine; influenzavirus; mouse model; neutralizing antibody; novel; pathogen; response; stem; transmission process; vaccine development

We have added Core E, to support a new joint Aim of the POI, described in both in the plans for the coming year in Project 3 and more briefly in Project 1. Dr. Kelsoe, the Pi of this Core, has been working closely with Haynes, Liao, and Harrison, and he is a coauthor (with Haynes, Harrison, and Kepler) ofthe review that describes (and names) B-cell lineage based immunogen design (Haynes, B.F., Kelsoe, G., Harrison, S.C, Kepler, T.B. B-cell-lineage immunogen design in vaccine development with HlV-1 as a case study. Nature Biotechnology 10: 423 (2012), PMC3512202). The novel, single-cell, B-cell culture system will allow us to test this notion on a feasible timescale in a tractable mouse model, and it will yield data that should facilitate more realistic models of the germinal center reaction and antibody affinity maturation (in humans as well as in mice) in the context of an influenza virus immunogen.

我们增加了核心E，以支持POI的新联合目标，在未来的计划中， 项目3中的一年，项目1中的一年更短。凯尔索博士，这个核心的Pi，一直在与 海恩斯，廖和哈里森，他是一个共同作者（与海恩斯，哈里森和开普勒）的评论， 描述了（和命名）基于B细胞谱系的免疫原设计（Haynes，B.F.，Kelsoe，G.，南卡罗来纳州哈里森 开普勒，TB疫苗开发中的B细胞谱系免疫原设计，以HlV-I作为案例研究。性质 Biotechnology 10：423（2012），PMC3512202）。这种新颖的单细胞B细胞培养系统将使我们能够 在一个易处理的小鼠模型中，在一个可行的时间尺度上测试这个概念，它将产生数据， 更现实的模型的germinal中心反应和抗体亲和力成熟（在人类以及 在小鼠中）。