Mitochondrial Biomarkers of Cardiomyopathy and Cure in Chagas Disease

描述（由申请人提供）：恰格巴氏心肌病（CCM）由原生动物克氏锥虫引起，是全球第三大热带疾病负担。据估计，美国有> 300，000名患者，流行国家有> 1000万名患者，查加斯病管理的年总成本估计为> 80亿美元。胆固醇血症和其他心血管疾病的共同特征是线粒体的功能变化，这是参与维持氧化磷酸化（OXPHOS）途径和线粒体生物发生的基因/蛋白质表达变化的结果。在这项提案中，我们将开发工具来捕获线粒体改变作为心脏病易感性和特定治疗效果（或毒性）的指标， 抑郁症患者。为此，我们将利用微粒（MP），其作为片段从真核细胞的质膜释放，并在细胞间通讯中发挥选择性作用;以及外周血单核细胞（即PBMC），其携带宿主的固有遗传特征，并反映身体的体内状态。使用这些容易获得的患者样本，我们将测试一种新的假设，即MP和PBMC携带CCM进展和治疗功效的特定特征，并且这些特征可以通过高通量体外筛选测定中线粒体生理学和生物发生的变化来捕获。支持我们假设的主要初步观察结果包括：（1）chagglutinase患者的心肌细胞和心脏活检组织中的DNA损伤与线粒体生物发生和OXPHOS途径的基因表达受损有关，（2）来自chagglutinase患者的MP在体外测定中影响线粒体功能和细胞活力， 以及（3）线粒体对药物的敏感性整合在整个细胞的背景下。我们将 采用PI实验室建立的尖端实验和高通量方法，以确定（1）MP是否携带MT生理学和生物发生的体内变化特征，并预测与CCM进展相关的分子过程，以及（2）基于MT的高通量筛选是否会捕获药物的作用，并有助于设计CCM的患者导向治疗。从目标1中MP诱导的线粒体反应和目标2中药物的线粒体毒性的协调分析和建模中获得的知识将导致改善对chagglutinase（和其他心肌病）患者的控制和治疗性治疗策略。相关性和创新：创新之处在于，我们的高通量方法将在DNA，RNA，蛋白质和功能水平上观察线粒体，并开发出对个性化医疗有价值的生物标志物概要，首先预测心脏病进展的风险，然后确定特定治疗是否会对个体产生不良影响或无效。重要的是，我们将开发的工具将适用于线粒体发挥作用的其他慢性疾病（例如，糖尿病、阿尔茨海默病、帕金森病、亨廷顿舞蹈症）和测试环境污染物的毒性。

DESCRIPTION (provided by applicant): Chagasic cardiomyopathy (CCM) is caused by the protozoan Trypanosoma cruzi, and represents the third greatest tropical disease burden globally. There are an estimated >300,000 patients in the US and >10 million patients in endemic countries, and total annual cost of Chagas disease management is estimated at >8 billion US dollars. A common feature of chagasic and other cardiovascular diseases is the functional changes in mitochondria as an outcome of changes in expression of genes/proteins involved in maintaining the oxidative phosphorylation (OXPHOS) pathway and mitochondrial biogenesis. In this proposal, we will develop tools to capture the mitochondrial alterations as an indicator of cardiac disease susceptibility and efficacy (or toxicity) of a particular treatment in chagasic patients. For this, we will utilize microparticles (MPs) that are released as fragments from the plasma membrane of eukaryotic cells, and play selective roles in intercellular communication; and peripheral blood mononuclear cells (i.e. PBMCs) that carry the inherent genetic signature of the host, and reflect the in vivo state of the body. Using these easily available patient samples, we will test a novel hypothesis that MPs and PBMCs carry the specific signature of CCM progression and the treatment efficacy, and these signatures can be captured via changes in mitochondrial physiology and biogenesis in high-throughput in vitro screening assays. The major preliminary observations supporting our hypothesis include (1) DNA damage in cardiomyocytes and heart biopsies of chagasic patients is associated with compromised mitochondrial biogenesis and gene expression for OXPHOS pathway, (2) MPs from chagasic patients influenced the mitochondrial function and cell viability in in vitro assays, and (3) mitochondrial sensitivity to drugs is integrated within the context of whole cells. We will employ cutting edge experimental and high-throughput methodologies, established in the PI's laboratory, to determine (1) whether MPs carry the signature of in vivo changes in mt physiology and biogenesis and predict the molecular processes associated with CCM progression, and (2) whether mt-based high-throughput screening will capture the drugs' effects and will be useful in designing the patient-oriented treatment for CCM. The knowledge gained from the coordinated analysis and modeling of MP-induced mitochondrial responses in aim 1 and mitochondrial toxicity of drugs in aim 2 will lead to improved control and therapeutic treatment strategies for chagasic (and other cardiomyopathy) patients. Relevance and innovation: The innovation lies in the idea that our high-throughput approach will look at mitochondria at the DNA, RNA, protein and functional levels and develop a compendium of biomarkers valuable in personalized medicine, for first predicting the risk of cardiac disease progression, and then determining if a particular treatment will have adverse effects or be inefficacious for an individual. Importantly, the tools we will develop will be applicable to other chronic diseases where mitochondria plays a role (e.g., diabetes, Alzheimer, Parkinson, Huntington) and to testing the toxicity of environmental pollutants.