CORE B: IN UTERO ELECTROPORATION CORE

该核心设施对计划项目的主要好处是，它为动物提供统一的外科手术， 和分子操控的能力。因此，将对动物进行治疗 从他们的母亲到达设施的时间到他们被运送到各个地方的时间， 项目此外，在这些动物中操纵的神经元将被用同样的方法完成。 shRNA和表达质粒的制备。这不仅从解剖学的角度来看很重要， 希望注射量和位置的个体间差异最小化，但 从动物的发展历史来看，一个特别重要的因素， 进行行为测试的动物。项目I和IH将使用活体动物， 大脑会被切到二号计划内核已经成功地在子宫内进行了这些操作 手术和shRNA设计和使用了7年。

The main benefit of this Core facility to the Program Project is that it provides animals with uniform surgical and molecular manipulations to all the Projects tnat require them. Thus, animals will be treated identically from the time of their mothers' arrival to the facility to the time they are shipped to various Projects. In addition, the neurons manipulated in these animals will have been done so with the same preparations of shRNA and expression plasmids. This is important not only from an anatomic perspective¿ where it is hoped that inter-individual variability in injection size and location is minimized¿but also from the point of view of the developmental history of the animal, an especially important factor for animals undergoing behavioral testing. Live animals will be used by Projects I and IH, and perfused brains will be snipped to Project II. The Core has been successfully performing these in utero electtoporations surgeries and shRNA design and use for 7 years.