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RNA Interference Core

RNA Interference Core
RNA干扰核心
批准号：
8604116
负责人：
AMY WOLVEN LASEK
金额：
$31.88万
依托单位：
UNIVERSITY OF ILLINOIS AT CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
项目状态：
已结题
起止时间：
2006-09-30 至 2016-08-31
来源：
https://reporter.nih.gov/project-details/8604116
关键词：
AddressAdenovirus VectorAdenovirusesAlcohol consumptionAnimal ModelAnimalsBehaviorBehavioralBrainBrain regionCandidate Disease GeneCellsCharacteristicsComplementary DNAEffectivenessGene ClusterGene ExpressionGene SilencingGene TargetingGenesGoalsHeavy DrinkingImmunohistochemistryIn VitroInjection of therapeutic agentLentivirus VectorMediatingMessenger RNAMolecularMonitorNeurobiologyPlasmidsProcessProteinsRNA InterferenceResearch PersonnelRodentRoleShippingShipsSignal PathwaySubfamily lentivirinaeTestingTimeTransfectionTransgenic AnimalsTransgenic MiceValidationViralViral VectorVirusWestern BlottingWorkbehavior testcDNA Expressiondesigndrinking behaviorin vivointerestknock-downlaser capture microdissectionmRNA Expressionoverexpressionprotein expressionscreeningsmall hairpin RNAtool

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英文摘要
DESCRIPTION (provided by applicant): This project will address the need of INIA-West for validating the role of INIA candidate genes in excessive drinking. The RNA Interference Core will provide viral vectors for RNA interference-mediated knockdown or over-expression of candidate genes to INIA-West Investigators. The specific aims of the project are to design short hairpin RNAs (shRNAs) targeting genes of interest and perform in vitro screening of shRNA efficacy, to produce virus expressing shRNAs for gene silencing in specific brain regions or in transgenic animals, to produce virus for over-expression of genes of interest, and to validate the effectiveness of gene silencing or over-expression in vivo. The viral tools developed by the INIA-West RNA Interference Core are crucial to understanding the role of specific genes in regulating excessive alcohol consumption.
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4/11 Neuroimmune and extracellular matrix interactions in alcohol consumption
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  • 财政年份：
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  • 依托单位：
    VIRGINIA COMMONWEALTH UNIVERSITY
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  • 批准号：
    10227050
  • 项目类别：
  • 资助金额：
    $39.94万
  • 财政年份：
    2019
  • 负责人：
    AMY WOLVEN LASEK
  • 依托单位：
    UNIVERSITY OF ILLINOIS AT CHICAGO
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  • 批准号：
    10732813
  • 项目类别：
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  • 财政年份：
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  • 负责人：
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  • 依托单位：
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