Novel therapeutic agents from the bacterial symbionts of Brazilian invertebrates

来自巴西无脊椎动物细菌共生体的新型治疗剂

• 批准号: 8785545
• 负责人: Cameron Robert Currie
• 金额: $ 7.61万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8785545
• 关键词: Actinobacteria class; Anti-Bacterial Agents; Antibiotics; Antifungal Agents; Ants; Bacteria; Bioinformatics; Biological Assay; Biological Factors; Brazil; Cataloging; Catalogs; Central America; Chagas Disease; Chemicals; Collaborations; Communicable Diseases; Ecology; Evolution; General Practitioners; Genomics; Geographic Distribution; Goals; Health; Hematopoietic Neoplasms; Human; Insecta; Invertebrates; Leishmaniasis; Mass Spectrum Analysis; Methods; Microbe; Parasites; Pattern; Pharmaceutical Preparations; Phylogenetic Analysis; Production; Research; Ribosomal RNA; Sampling; Sequence Analysis; Soil; Source; Specificity; Streptomyces; Students; Symbiosis; System; Technology; Testing; Therapeutic; Therapeutic Agents; Training; Transcription Factor AP-1; Universities; Wisconsin; Work; base; experience; fungus; in vivo imaging; insight; interest; microbial; next generation sequencing; novel; novel therapeutics; pathogen; rRNA Genes; small molecule; social; success

PROJECT SUMMARY/ABSTRACT Discovering novel natural products that can serve as drugs or drug leads is of critical importance to human health. Our recent work has identified that bacterial symbionts of insects represents a rich, diverse, and largely untapped source of novel compounds. This ICBG focuses on identifying novel natural products from symbiotic bacteria associated with fungus-growing ants and other social insects in Brazil. We will also determine their potential to treat fungal pathogens, Chagas disease, leishmaniasis, and cancer of the blood. In AP3, we will contribute to the overall goals of this ICBG in a number of ways. Working closely with AP1, we will conduct intensive sampling of bacterial symbionts from insects collected from a range of biomes in Brazil. Strains will be catalogue and their 16S rRNA sequenced. Using multilocus sequence typing, we will examine the diversity and host specificity of strains. For 500 strains per year identified to be of highest interest, we will determine both their secondary metabolite potential as well as the ecological context associated with their ability to produce natural products. To do this, we will perform ecological assays between bacterial symbionts and fungal antagonists associate with their insect host, conduct genomic sequencing and analyses, and do in vivo imaging mass spectrometry. Finally, we will provide training to young biologists in fieldwork on bacterial symbionts of insects, tropical microbial ecology, and bioinformatics. Training will include hosting Brazilian students at the University of Wisconsin-Madison, as well as training students from US labs on conducting field research in Brazil.

项目总结/文摘