Direct single nucleotide mapping of genomic CpG marks

基因组 CpG 标记的直接单核苷酸作图

• 批准号: 8651509
• 负责人: Saulius Klimasauskas
• 金额: $ 13.28万
• 依托单位: VILNIAUS UNIVERSITETAS
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-04-12 至 2016-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8651509
• 关键词: Area; Asthma; Autistic Disorder; Boxing; Brain; Cells; Complex; CpG dinucleotide; Cytosine; DNA; DNA Methylation; DNA Structure; DNA biosynthesis; DNA-Directed DNA Polymerase; Diabetes Mellitus; Economics; Epigenetic Process; Generations; Genome; Genomic DNA; Genomics; Human; Individual; Invaded; Malignant Neoplasms; Mammals; Maps; Methods; Methyltransferase; Modification; Morphologic artifacts; Mus; Names; Neurons; Nucleotide Mapping; Nucleotides; Oxygenases; Pattern; Polymerase; Positioning Attribute; Process; Protocols documentation; Pyrimidine; Reaction; Regulation; Research; Resolution; Role; Sampling; Schizophrenia; Signal Transduction; Site; Techniques; base; bisulfite; cost effective; demethylation; embryonic stem cell; epigenome; epigenomics; genome sequencing; genome-wide; genome-wide analysis; human disease; human tissue; mammalian genome; new technology; public health relevance; single molecule; tool

DESCRIPTION (provided by applicant): Over the past decade, epigenetic phenomena claimed a central role in cell regulatory processes and proved important factors for understanding complex human diseases. One of the best understood epigenetic mechanisms is DNA methylation. In the mammalian genome, cytosines (C) were long known to exist in two functional states: unmethylated or methylated at the 5-position of the pyrimidine ring (5mC). Recent studies of genomic DNA from the human and mouse brain, neurons and from mouse embryonic stem cells found that a substantial fraction of 5mC in CpG dinucleotides is converted to 5-hydroxymethylcytosine (hmC) by the action of oxygenases. These findings provided important clues in a long elusive mechanism of active DNA demethylation and bolstered a fresh wave of studies in the area of epigenetic regulation in mammals. However, such studies are hindered by the shortcomings of available experimental techniques for genome-wide epigenome analysis. This project is dedicated to developing a break-through technique for genome-wide analysis of the modification status of CpG dinucleotides that combines single-base resolution with targeted and economic sequencing of the genome.

描述（由申请人提供）： 在过去的十年中，表观遗传现象声称在细胞调控过程中发挥了核心作用，并证明了理解复杂人类疾病的重要因素。最好理解的表观遗传机制之一是DNA甲基化。在哺乳动物基因组中，胞嘧啶（C）长期以来已知以两种功能状态存在：未甲基化或在嘧啶环的5位甲基化（5 mC）。最近对来自人类和小鼠脑、神经元和小鼠胚胎干细胞的基因组DNA的研究发现，CpG二核苷酸中的5 mC的大部分通过加氧酶的作用转化为5-羟甲基胞嘧啶（hmC）。这些发现为长期难以捉摸的主动DNA去甲基化机制提供了重要线索，并支持了哺乳动物表观遗传调控领域的新一轮研究。然而，这种研究受到现有全基因组表观基因组分析实验技术的缺点的阻碍。该项目致力于开发一种突破性的技术，用于对CpG二核苷酸的修饰状态进行全基因组分析，该技术将单碱基分辨率与基因组的靶向和经济测序相结合。