The Safety of Channel Catfish Pituitary Administered via Intraperitoneal Injectio

斑点叉尾鮰垂体腹腔注射的安全性

• 批准号: 8722120
• 负责人: Patricia Simmons Gaunt
• 金额: $ 4.48万
• 依托单位: MISSISSIPPI STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-02-01 至 2017-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8722120
• 关键词: Safety; Channel; Catfish; Pituitary; Administered

Project Summary The use of hormones to induce spawning in fish is critical to the success of many U.S hatchery/fisheries programs. During the course of normal spawning operations at a hatchery, it may be necessary to handle and examine individual fish weekly over a 6 - 8 week period. Such procedures can be extremely stressful to valuable brood stock, and compromise their health. Successful hormone treatment can reduce handling requirements to a single hormone administration followed by gamete collection, thereby greatly reducing overall fish handling. Common carp pituitary (CCP) is the most widely used drug to induce final gamete maturation (spawning) in fish. Channel Catfish Pituitary (CP) has also been found to be similarly effective in inducing ovulation and spermiation. Although CP has not been widely used to date, it is readily available in the MS Delta. The US catfish industry is interested in obtaining a CP label to induce final gamete maturation in channel catfish (Icatlurus punctatus) for the production of hybrid catfish (Ictalurus punctatus x Ictalurus furcatus). The specific objective of the proposed project is to evaluate the safety of CP administered via intraperitoneal (IP) injection to adult female channel catfish (Ictalurus punctatus)/group at 0¿, 1¿ (12 mg), 2¿ (24 mg), and 3¿ (36 mg) the maximum proposed efficacious dose of CP/kg fish body weight (bw). Ten fish per group will be injected on Days 1 and 2. On Day 3 they will be strip spawned then humanely euthanized. The egg quality will be evaluated and the fish evaluated by pathology and histopathology. The study will be conducted at the Thad Cochran National Warmwater Aquaculture Center Wet Lab in Stoneville MS. The study will be conducted here because the facility has (1) a ready accessible source of channel catfish brood stock (2) sufficient resources to conduct the study e.g. space, water, tanks, necropsy and histopathology facilities, as well as personnel to administer treatment and collect required data) and (3) demonstrated commitment to adhere to the study protocol and other relevant guidelines. The study will be conducted under an FDA CVM accepted pivotal Target Animal Safety (TAS) study protocol (Study Protocol Number CP-12-TAS-CCF) and is intended to provide FDA CVM with sufficient data to complete the TAS portion requirements for approval of CP use in channel catfish. Ultimately the data generated in the study will be used to support a supplemental New Animal Drug Application for the use of CP as a spawning aid in female channel catfish.

项目摘要 使用激素诱导鱼类产卵是许多美国鱼类成功的关键 孵化场/渔业项目。在孵化场的正常产卵过程中，它可能 有必要在6-8周内每周处理和检查每条鱼。是这样的 手术可能会给宝贵的幼仔带来极大的压力，并危及它们的健康。 成功的激素治疗可以将操作要求减少到一次激素注射。 随之而来的是配子收集，从而大大减少了整体鱼类处理。鲤鱼脑下垂体 CCP是最广泛使用的诱导鱼类最终配子成熟(产卵)的药物。渠道 研究还发现，叉尾鱼脑下垂体(CP)在诱导排卵和 受精。尽管CP到目前为止还没有被广泛使用，但它在MS Delta中很容易得到。 美国鲶鱼业对获得CP标签以诱导最终配子成熟感兴趣 斑点叉尾鱼 Ictalurus Furcatus)。 拟议项目的具体目标是评估通过以下途径给予的CP的安全性 成年雌性斑点叉尾鱼(Ictalurus Marktatus)腹腔注射(Ip)0°，1°(12)组 Mg)、2？(24毫克)和3？(36毫克)每公斤鱼体重中CP的最大建议有效剂量 (BW)。在第1天和第2天，每组注射10条鱼。在第3天，它们将被剥离产卵 人道的安乐死。将对鸡蛋质量进行评估，并通过病理和组织学对鱼类进行评估 组织病理学。 这项研究将在Thad Cochran国家Warmwater水产养殖中心进行 密西西比州斯通维尔的湿实验室研究将在这里进行，因为该设施有(1)准备好 可获得的斑点叉尾鱼鱼种来源(2)有足够的资源进行研究，例如 空间、水、坦克、尸检和组织病理学设施以及管理人员 治疗和收集所需数据)和(3)表明对坚持研究的承诺 协议和其他相关指南。 这项研究将在FDA CVM认可的Pivotal Target动物安全(TAS)下进行 研究协议(研究协议编号CP-12-TAS-CCF)，旨在向FDA CVM提供 足够的数据完成TAS部分的要求，以批准在斑点叉尾鱼中使用CP。 最终，研究中产生的数据将被用来支持一种补充的新动物药物 CP作为雌性斑点叉尾鱼产卵助产剂的应用