Mouse Genetics Core

小鼠遗传学核心的目的是提供集中育种，基因分型和质量 控制转基因和基因敲除动物，供研究人员在项目中使用。此外，本发明还提供了一种方法， 核心将产生项目2所需的新的转基因小鼠模型。核心还将提供一个 新的实验性转基因模型分发到国家储存库（如 杰克逊实验室诱导的突变资源），如我们的共享计划中所述，或其他 研究人员在爱荷华大学，威尔康奈尔医学院和外部机构。的 核心的主要职责是：a）保持遗传种群，B）育种和基因分型 转基因和基因敲除动物，c）转基因小鼠的产生，d）提供实验和 对研究者控制小鼠，e）动物使用的统一机构认证，f）质量控制。所有的 该计划中的项目将广泛使用基因操纵小鼠作为主要的 实验平台核心叙述提供了这些菌株的全面列表。模型 由爱荷华州或WCMC的调查人员制作，从国家知识库（即 杰克逊实验室），或从合作者和顾问处获得。小鼠遗传学核心将 用于集中维护转基因和基因敲除小鼠的遗传储备， 节目中的调查员。核心主任将监督动物护理人员在所有的设置 育种，并将负责保持遗传纯度和质量控制。基因分型 保护动物是内核的主要职责之一将进行基因分型， 目的：a）在生殖系传递小鼠中鉴定来自野生型的杂合子，B）鉴定 转基因建立者和后代，c）鉴定通过杂交育种模型产生的完整模型， d）与C57 BL/6回交的基础和生产菌落，和e）质量控制。 相关性（参见说明）： 核心C使命是促进整个PPG开展的研究，并通过以下方式加速发现 为开发、获取和基因分型简单和复杂的 小鼠模型。

The purpose of the mouse genetics core is to provide centralized breeding, genotyping and quality control of transgenic and knockout animals for use by investigators in the program project. In addition, the core will generate new transgenic mouse models needed by Project 2. The core will also provide a mechanism for distribution of new experimental transgenic models to either a national repository (such as the Jackson Laboratory Induced Mutant Resource), as described in our sharing plan, or to other investigators at the University of lowa, Weill Cornell Medical College and outside of either institution. The main responsibilities of the Core will be: a) maintenance of genetic stocks, b) breeding and genotyping transgenic and knockout animals, c) generation of transgenic mice, d) provision of experimental and control mice to investigators, e) unified institutional accreditation for animal use, f) quality control. All the projects in this program will make extensive use of genetically manipulated mice as the primary experimental platform. A comprehensive list of these strains is provided the core narrative. The models were either made by investigators at lowa or WCMC, were obtained from a National Repository (i.e. the Jackson Laboratory), or were obtained from collaborators and consultants. The mouse genetics core will be used to centralize the maintenance of genetic stocks of transgenic and knockout mice used by investigators in the program. The core director will oversee the animal care staff in the setup of all breeding and will be responsible for the maintenance of genetic purity and quality control. The genotyping of animals is one of the primary responsibilities of the Core. Genotyping will be performed for the purposes of: a) identifying heterozygotes from wild type among germline transmission mice, b) identifying transgenic founders and offspring, c) identifying complete models generated by crossbreeding models , d) foundation and production colonies backcrossed onto C57BL/6, and e) quality control. RELEVANCE (See instructions): Core C mission is to facilitate research carried out by the entire PPG and to accelerate discovery by providing a seamless pipeline for the development, acquisition and genotyping of simple and complex mouse models.