Mouse Genetics Core

小鼠遗传学核心

• 批准号: 8651941
• 负责人: Justin L Grobe
• 金额: $ 15.07万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8651941
• 关键词: Accreditation; Animals; Backcrossings; Breeding; Complex; Crossbreeding; Development; Foundations; Generations; Genetic; Genotype; Heterozygote; Institution; Instruction; Knockout Mice; Maintenance; Mission; Modeling; Mus; Production; Quality Control; Research; Research Personnel; Resources; Signal Transduction; The Jackson Laboratory; Transgenic Animals; Transgenic Mice; Transgenic Model; Transgenic Organisms; Universities; animal care; blood pressure regulation; knockout animal; medical schools; mouse model; mutant; offspring; programs; repository; transmission process

The purpose of the mouse genetics core is to provide centralized breeding, genotyping and quality control of transgenic and knockout animals for use by investigators in the program project. In addition, the core will generate new transgenic mouse models needed by Project 2. The core will also provide a mechanism for distribution of new experimental transgenic models to either a national repository (such as the Jackson Laboratory Induced Mutant Resource), as described in our sharing plan, or to other investigators at the University of lowa, Weill Cornell Medical College and outside of either institution. The main responsibilities of the Core will be: a) maintenance of genetic stocks, b) breeding and genotyping transgenic and knockout animals, c) generation of transgenic mice, d) provision of experimental and control mice to investigators, e) unified institutional accreditation for animal use, f) quality control. All the projects in this program will make extensive use of genetically manipulated mice as the primary experimental platform. A comprehensive list of these strains is provided the core narrative. The models were either made by investigators at lowa or WCMC, were obtained from a National Repository (i.e. the Jackson Laboratory), or were obtained from collaborators and consultants. The mouse genetics core will be used to centralize the maintenance of genetic stocks of transgenic and knockout mice used by investigators in the program. The core director will oversee the animal care staff in the setup of all breeding and will be responsible for the maintenance of genetic purity and quality control. The genotyping of animals is one of the primary responsibilities of the Core. Genotyping will be performed for the purposes of: a) identifying heterozygotes from wild type among germline transmission mice, b) identifying transgenic founders and offspring, c) identifying complete models generated by crossbreeding models , d) foundation and production colonies backcrossed onto C57BL/6, and e) quality control. RELEVANCE (See instructions): Core C mission is to facilitate research carried out by the entire PPG and to accelerate discovery by providing a seamless pipeline for the development, acquisition and genotyping of simple and complex mouse models.

小鼠遗传学核心的目的是提供集中的育种、基因分型和质量 控制转基因和基因敲除动物，供项目研究人员使用。此外, 该核心将产生项目2所需的新的转基因小鼠模型。该核心还将提供 将新的实验性转基因模型分发到国家资源库(如 杰克逊实验室诱导的突变资源)，如我们的共享计划中所述，或向其他 爱荷华大学、威尔·康奈尔医学院以及这两所学院以外的研究人员。这个 核心的主要责任将是：a)维持遗传储备，b)育种和基因分型 转基因和基因敲除动物，c)转基因小鼠的产生，d)提供实验和 控制老鼠给调查人员，e)统一动物使用的机构认证，f)质量控制。所有的 该计划中的项目将广泛使用转基因小鼠作为主要的 实验平台。核心叙述中提供了这些压力的全面清单。模特们 是由爱荷华州或WCMC的调查人员制作的，是从国家资料库(即 杰克逊实验室)，或从合作者和顾问那里获得。小鼠的遗传核心将 用于集中维护转基因和基因敲除小鼠的遗传库存 该项目中的调查人员。核心主任将监督所有动物护理人员的设置 并将负责保持遗传纯度和质量控制。基因分型 保护动物是核心组织的主要职责之一。将对这些患者进行基因分型 目的：a)在生殖系传播的小鼠中识别杂合子和野生型，b)识别 转基因创始人和后代，c)鉴定杂交模型产生的完整模型， D)基础菌落和生产菌落回交到C57BL/6，以及e)质量控制。 相关性(请参阅说明)： 核心C任务是促进整个PPG进行的研究，并通过以下方式加速发现 为简单和复杂基因的开发、获取和基因分型提供无缝管道 老鼠模型。