On-the-Fly Field-potential Sensing Electrode Track based NSC sorting for brain re

基于动态场电位传感电极轨迹的 NSC 脑再分类

• 批准号: 8712598
• 负责人: John Collins
• 金额: $ 35万
• 依托单位: BIOPICO
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-05 至 2016-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8712598
• 关键词: Affect; Algorithms; Alzheimer' s Disease; American; Amplifiers; Amyotrophic Lateral Sclerosis; Area; Arizona; Asses; Biological Assay; Brain; California; Cell Separation; Cell Signaling Process; Cell Therapy; Cell model; Cells; Cellular Morphology; Clinical; Clinical Laboratory Techniques; Complex; Computer software; County; Databases; Derivation procedure; Detection; Development; Differentiation Antigens; Direct Costs; Disease; Electrodes; Family; Feasibility Studies; Fluorescent Dyes; Gene Expression; Genetic; Genomics; Geometry; Glass; Goals; Health; Height; Human; Huntington Disease; Individual; Label; Laboratories; Lead; Lesion; Manuals; Measurement; Mental disorders; Methods; Metric; Microelectrodes; Microfluidics; Microscope; Modification; Morphologic artifacts; Neuraxis; Neurodegenerative Disorders; Neurons; Neurosciences; Noise; Optics; Oranges; Organ; Parkinson Disease; Patients; Pediatric Hospitals; Performance; Phase; Pluripotent Stem Cells; Polymers; Productivity; Protocols documentation; Pump; Quantitative Reverse Transcriptase PCR; Regenerative Medicine; Replacement Therapy; Research; Research Institute; Research Personnel; Response to stimulus physiology; Risk; Sampling; Signal Transduction; Somatic Cell; Sorting - Cell Movement; Specificity; Spinal cord injury; Stem cells; Sterility; Stimulus; Stroke; System; Techniques; Technology; Teratoma; Therapeutic; Time; Tissues; Translating; Translations; Undifferentiated; Universities; Validation; Width; abstracting; base; brain research; brain tissue; cell type; clinical application; cost; design; electric impedance; epigenetic marker; fluorexon; fly; human disease; improved; induced pluripotent stem cell; nanosystems; nerve stem cell; nervous system disorder; novel; pluripotency; prevent; programs; prototype; public health relevance; remyelination; research study; response; simulation; stem cell biology; tool; verification and validation

DESCRIPTION (provided by applicant): "'On-the-Fly Field-potential Sensing Electrode Track' (OFFSET) based NSC sorting for brain research". Abstract: The past decade has acquired a profound paradigm shift in high throughput tools for brain research with the advent of programming techniques in micro/nano systems, stem cells, signal processing and genomics. One area in which these technologies have opened new avenues has been the application of pluripotent stem cells to study neurodegenerative diseases. The derivation of patient-specific reprogrammed somatic cells enables immunologically compatible viable brain tissue for use in studying disease development, creating therapies for neurological disorders and developing perfect models for the cells of the central nervous system that are harmed in the diseases. The major challenge in translating stem cell biology into such useful tissue is the establishment of effective separation methods to isolate differentiated cells and exclude cells that hinder graft performance or lead to teratoma formation. Unfortunately, conventional separation techniques for stem cells such as microscope-assisted manual isolation, FACS and MACS require intensive labor, exogenous labeling or genetic modification is not suitable for such clinical applications. Therefore, Biopico Systems teams with the researchers from University of California, Irvine for their expertise in microfluidics, the Children's Hospital of Orange County (CHOC) Research Institute for their expertise in iPS cells therapy, and Arizona State University for their expertis in neuronal stimulus recording using microelectrode array in order to demonstrate the technical feasibility of a label-free electrical field potential marker based cell sorting for brain research With our understanding of the practical constraints of the system components from the laboratories of our collaborators, we propose to develop complex therapeutic technology for research and practice. We envision developing a family of systems to sort neural stem cells for several applications to lead technological transformation in neuroscience.

描述（由申请人提供）：“用于脑研究的基于NSC分选的‘即时场电位感测电极轨迹’（OFFSET）”。摘要：在过去的十年中，随着微/纳米系统、干细胞、信号处理和基因组学中编程技术的出现，大脑研究的高通量工具发生了深刻的范式转变。这些技术开辟了新途径的一个领域是应用多能干细胞研究神经退行性疾病。患者特异性重编程体细胞的衍生使免疫相容的活脑组织能够用于研究疾病发展，创造神经系统疾病的治疗方法，并为在疾病中受损的中枢神经系统细胞开发完美的模型。将干细胞生物学转化为这种有用的组织的主要挑战是建立有效的分离方法来分离分化的细胞并排除阻碍移植物性能或导致畸胎瘤形成的细胞。遗憾的是，用于干细胞的常规分离技术如显微镜辅助的手动分离、FACS和MACS需要密集的劳动，外源标记或遗传修饰不适合于此类临床应用。因此，Biopico Systems与来自加州大学欧文分校的研究人员合作，他们在微流体方面的专业知识，橙子县儿童医院（CHOC）研究所在iPS细胞治疗方面的专业知识，和亚利桑那州州立大学，他们在使用微电极阵列记录神经元刺激方面的专业知识，以证明标记的技术可行性，基于自由电场电位标记的细胞分选用于脑研究随着我们对合作者实验室系统组件的实际限制的理解，我们建议开发用于研究和实践的复杂治疗技术。我们设想开发一系列系统来对神经干细胞进行分类，以应用于神经科学的技术转型。