Preclinical Optical Imaging System Scintillator and Pinhole Insert

临床前光学成像系统闪烁体和针孔插入物

• 批准号: 8823458
• 负责人: Gregory Scott Mitchell
• 金额: $ 28.56万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-25 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8823458
• 关键词: Animal Model; Animals; Area; Basic Science; Biological Process; Biology; Bioluminescence; Caliber; Cherry - dietary; Collimator; Computer software; Consult; Data; Data Analyses; Deposition; Development; Disease model; Evaluation; Event; Experimental Designs; Exposure to; Film; Functional Imaging; Gamma Rays; Geometry; Goals; Height; Image; Imaging Phantoms; Imaging Techniques; Institution; Kidney; Light; Methods; Modality; Monte Carlo Method; Morphology; Nuclear; Optics; Performance; Pharmaceutical Preparations; Photons; Positron-Emission Tomography; Production; Property; Protocols documentation; Radiation; Radiation Monitoring; Radioisotopes; Relative (related person); Research; Research Personnel; Resolution; Running; Scanning; Scanning Electron Microscopy; Simulate; Speed; Structure; System; Systems Development; Testing; Thick; Thyroid Gland; Tomography, Computed, Scanners; Visible Radiation; Work; absorption; attenuation; base; charge coupled device camera; clinical practice; cost; design; detector; flexibility; fluorescence imaging; high throughput screening; human disease; imaging probe; improved; instrumentation; light emission; light transmission; molecular imaging; monitoring device; novel; novel therapeutics; optical imaging; pre-clinical; public health relevance; radiotracer; simulation; single photon emission computed tomography; tool; transmission process; uptake

DESCRIPTION (provided by applicant): Preclinical molecular imaging is used in studying animal models of human disease, evaluating new therapeutic drugs, and in carrying out research in basic biology. Optical imaging techniques, such as bioluminescence and multispectral fluorescence imaging, currently are widely used for preclinical functional imaging despite their depth dependent limitations on quantitation, resolution and sensitivity. Nuclear imaging techniques, SPECT and PET, are more closely translational. Dedicated preclinical SPECT and PET systems provide quite good imaging capability, but can be expensive, difficult (for non-experts), and slow to use. Optical systems are widely available, relatively affordable, easy to operate, and quick (for setup, imaging, and data analysis; i.e. high-throughput). For some imaging tasks a dedicated SPECT system would be essential, but for many studies a high-throughput acquisition of projection images would be sufficient and gains from cost reduction and speed could be significant. High resolution planar images of gamma-ray emitting radiotracers can be obtained by retrofitting a small animal optical imaging system (such as an IVIS Spectrum (Perkin-Elmer)) with a pinhole collimator and a large area CsI:Tl scintillator with a novel morphology. Recent progress at Radiation Monitoring Devices (RMD) has led to thick, transparent, crystalline microcolumnar structure (CMS) scintillator detectors of CsI(Tl) that simultaneously provide high gamma-ray absorption efficiency, high intrinsic spatial resolution, and bright light emission. The ability to use an existing commercial preclinical optical imaging system to rapidly acquire planar gamma ray images with good spatial resolution of one or possibly multiple animals would be a new and useful tool for high throughput screening of molecular imaging probes. Adding a nuclear imaging capability to the many existing preclinical optical imaging systems is an appealing opportunity for greatly expanded access to that modality of molecular imaging. The goal of this proposal is to build and characterize an insert which can be placed in an existing optical imaging system, where the insert uses a collimator and scintillator to enable gamma rays to be imaged by the existing sensitive CCD camera. The collimators used will be from a commercial SPECT imaging system. In this project we will: (1) develop protocols for production of large (15 cm diameter) thick (up to 4 mm) CMS CsI(Tl) films with improved light transmission; (2) simulate collimator and detector configurations using the GATE software package; (3) produce three large CMS detectors for use in an optical system insert; (4) fabricate an insert with flexible geometry for a variety of collimator and scintillator configurations, and perform phantom imaging studies to characterize the system; and (5) conduct two proof-of-concept animal imaging studies to demonstrate the potential for dynamic imaging and for high resolution imaging of a smaller field of view.

描述（申请人提供）：临床前分子成像用于研究人类疾病的动物模型，评估新的治疗药物，并进行基础生物学研究。光学成像技术，如生物发光和多光谱荧光成像，目前被广泛用于临床前功能成像，尽管它们的深度依赖于定量，分辨率和灵敏度的限制。核成像技术，SPECT和PET，是更密切的翻译。专用的临床前SPECT和PET系统提供了相当好的成像能力，但是可能是昂贵的、困难的（对于非专家）并且使用缓慢。光学系统是广泛可用的，相对负担得起，易于操作，快速（用于设置，成像和数据分析;即高通量）。对于某些成像任务，专用SPECT系统将是必不可少的，但对于许多研究，投影图像的高通量采集将是足够的，并且从成本降低和速度中获得的收益可能是显著的。γ射线发射放射性示踪剂的高分辨率平面图像可以通过改装具有针孔准直器的小动物光学成像系统（诸如IVIS Spectrum（Perkin-Elmer））和具有微透镜的大面积CsI：Tl闪烁体来获得。 新形态学辐射监测装置（RMD）的最新进展已经导致CsI（Tl）的厚的、透明的、结晶微柱结构（CMS）闪烁体探测器，其同时提供高伽马射线吸收效率、高固有空间分辨率和明亮的光发射。使用现有的商业临床前光学成像系统快速获取一个或可能多个动物的具有良好空间分辨率的平面伽马射线图像的能力将是用于分子成像探针的高通量筛选的新的有用工具。将核成像能力添加到许多现有的临床前光学成像系统是极大地扩展对该分子成像模态的访问的有吸引力的机会。本提案的目标是构建和表征可以放置在现有光学成像系统中的插入物，其中插入物使用准直器和闪烁体来使伽马射线能够由现有灵敏CCD相机成像。使用的准直器将来自商业SPECT成像系统。在这个项目中，我们将：（1）开发用于生产具有改进的光透射率的大（15 cm直径）厚（高达4 mm）CMS CsI（Tl）膜的方案;（2）使用GATE软件包模拟准直器和检测器配置;（3）生产用于光学系统插入物的三个大CMS检测器;（4）制造具有用于各种准直器和闪烁体的灵活几何形状的插入物 配置，并执行体模成像研究以表征系统;以及（5）进行两项概念验证动物成像研究，以证明动态成像和较小视场高分辨率成像的潜力。