Functional connectivity of isolated neuronal circuits in primary visual cortex

初级视觉皮层中孤立神经元回路的功能连接

• 批准号: 8784091
• 负责人: Esther Richler
• 金额: $ 5.33万
• 依托单位: SALK INSTITUTE FOR BIOLOGICAL STUDIES
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-16 至 2017-09-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8784091
• 关键词: Address; Affect; Animals; Antibodies; Attention; Autistic Disorder; Autopsy; Biological Neural Networks; Brain; Calcium; Cells; Cerebral cortex; Characteristics; Complex; DNA; Data; Disease; Distant; Electroporation; Frequencies; Functional disorder; Health; Image; In Vitro; Infection; Interneurons; Label; Life; Light; Link; Location; Maps; Measures; Mental Depression; Methodology; Methods; Mus; Nervous System Physiology; Neuromuscular Diseases; Neurons; Output; Paralysed; Parkinson Disease; Parvalbumins; Pattern; Photic Stimulation; Play; Population; Positioning Attribute; Process; Property; Publishing; Rabies; Rabies virus; Research; Rodent; Role; Schizophrenia; Sensory; Shapes; Somatostatin; Source; Staining method; Stains; Structure; Synapses; System; Testing; Visual; Visual Cortex; Work; area striata; base; cell type; design; excitatory neuron; hippocampal pyramidal neuron; in vivo; information processing; inhibitory neuron; insight; novel; orientation selectivity; postsynaptic; preference; presynaptic; public health relevance; receptive field; research study; response; two-photon; visual information; visual stimulus

DESCRIPTION (provided by applicant): The function of the nervous system is dependent on complex interactions between networks of neurons. Understanding how these networks function in both health and disease is dependent on understanding the function of the fine-scale connections between neurons. The research proposed here is aimed at revealing the functions of specific connections between similar and different types of neurons in the same or different cortical layer. A rabies-based tracing system will reveal the direct monosynaptic connections between neurons in the primary visual cortex of mice. Visual stimulation combined with calcium imaging will then reveal the visual response properties of connected neurons. This method will be the first to simultaneously assess both connectivity and function in a live animal without limitations on the distance between connected cells. The cortical sources and functional contributions of excitatory and inhibitory inputs to single functionally characterized excitatory neurons will be uncovered, and the principles by which a single neuron integrates many inputs to produce a single functional output will be studied. The results of these experiments will shed light on various hypotheses about the functional roles of fine-scale connections in cortical information processing.

描述(申请人提供)：神经系统的功能依赖于神经元网络之间的复杂相互作用。要了解这些网络如何在健康和疾病中发挥作用，取决于了解神经元之间的细微联系的功能。这项研究旨在揭示相同或不同皮质层中相似和不同类型神经元之间特定联系的功能。一个基于狂犬病的追踪系统将揭示小鼠初级视皮层神经元之间的直接单突触联系。视觉刺激结合钙离子成像将揭示连接神经元的视觉反应特性。这种方法将首次同时评估活体动物的连接性和功能，而不受连接细胞之间距离的限制。兴奋性和抑制性输入对单个功能特征的兴奋性神经元的皮质来源和功能贡献将被揭示，并将研究单个神经元整合多个输入以产生单一功能输出的原理。这些实验的结果将阐明关于细小尺度连接在皮层信息处理中的功能作用的各种假说。