Vector Core

爱荷华大学的载体核心集成到多个基因治疗项目中，旨在研究肺、眼、脑、肝、心脏和肌肉的疾病，以及心血管和神经肌肉系统的研究。此外，在心血管中心、黄斑变性中心和囊性纤维化中心使用的基因转移技术的开发中，矢量核心公司的工作人员也是积极的参与者。由此产生的与来自不同学科的多名研究人员的互动允许思想、技术进步和载体设计方面的创新。载体核心设施的总体目标是支持研究人员使用基因转移技术。这包括与首席调查员和工作人员协商，开发新的载体，为功能和纯度而产生的载体的协作测试，以及载体的常规准备(包括质量控制)。在媒介设计和生成的所有阶段，媒介核心工作人员和调查人员都保持着密切联系。因此，核心既是基因转移研究的研究和开发设施，也是常规载体准备的服务设施。作为其服务的一部分，载体核心提供重组腺病毒、腺相关病毒(AAV)和逆转录病毒(包括慢病毒)的纯化和浓缩制剂。该设施还将提供获得标准细胞系、表达质粒和重组报告病毒的途径。 核心小组的主要职责是： 重组载体的制备 ？传播媒介 ？维护现有媒介种群数据库 维护现有表达载体数据库；开发新的表达载体 发展新的病毒生产方法 ·就新媒介的设计和开发提供咨询和援助

Vector Core The Vector Core at the University of Iowa is integrated into multiple gene therapy projects directed at the study of diseases of the lung, eye, brain, liver, heart and muscle, as well as at the study of the cardiovascular and neuromuscular systems. Also, Vector Core staff are active participants in the development of gene transfer technologies used by the Cardiovascular, Macular Degeneration, and Cystic Fibrosis Centers. The resulting interaction with multiple investigators from various disciplines allows for cross-fertilization of ideas, technical advancements, and innovations in vector designs. The overall objective of the Vector Core facility is to support investigators in the use of gene transfer technologies. This includes consultation with the Principle Investigator and staff, development of novel vectors, collaborative testing of the vectors generated for function and purity, and routine preparation of vectors (including quality control). The Vector Core staff and investigators are in close contact through all phases of vector design and generation. Thus, the Core serves both as a research and development facility for gene transfer studies and a service facility for routine vector preparation. As a part of its service, the Vector Core provides purified and concentrated preparations of recombinant adenovirus, adeno-associated virus (AAV), and retrovirus (including lentivirus). This facility will also provide access to standard cell lines, expression plasmids, and stocks of recombinant reporter viruses. The main responsibilities of the Core will be: ¿ Preparation of recombinant vectors ¿ Dissemination of vectors ¿ Maintainance of database of available vector stocks ¿ Maintainance of database of available expression vectors; development of new expression vectors ¿ Development of novel methods for virus production ¿ Consultation on and assistance in the design and development of novel vectors