Cytometry Reagent Database and Automated Panel Design Software.

描述（由申请人提供）：本拨款申请中描述的拟议项目，结合现有的Fluorish工具，将创建一个强大的基于云的细胞分析研究试剂电子商务市场，解决研究界的关键需求。流式细胞术广泛应用于基础和临床研究。fluish LLC将简化实验设计过程，提高流式细胞术研究的科学探究质量。通过迭代开发，我们将开始实现一个自动化的面板设计系统，以及一个全面的试剂和面板数据库。将云中的这些组件与Fluorish的社会计算能力相连接，将帮助用户在多参数流式细胞术实验设计中追求更大的复杂性，并显着增强他们的科学探索。

DESCRIPTION (provided by applicant): The proposed project described in this grant application, combined with existing Fluorish tools, will create a robust cloud-based e-commerce marketplace for cytometry research reagents, addressing key needs in the research community. Flow Cytometry is widely used in basic and clinical research. Fluorish LLC will streamline the experimental design process and enhance the quality of scientific inquiry for flow cytometry research. Through iterative development, we will begin implementing an automated panel design system, and a comprehensive reagent and panel database. Linking these components in the cloud with Fluorish's social computing capabilities, will help users pursue greater complexity in multiparametric flow cytometry experimental design and significantly enhance their scientific exploration. Objectives: {(1) Provide an online cloud environment that allows researchers to develop valuable social networks, while integrating robust scientific experimental design tools, with core/lab management systems, and an e-commerce online marketplace.} (2) Develop and implement an experimental design schema, with integrated evaluation metrics, that will automatically characterize the interaction between reagent and instrument dependencies, to account for the complexities of fluorophore assignments in panel design. (3) Create heuristics with a common ontology for flow cytometry reagents and panels that will allow for greater transparency on use of reagents, and sharing capabilities of optimized flow cytometry panels. Methods: Building on our existing core and lab management systems, we will continue to add functionality to our site, allowing scientists to readily manage and distribute critical informatio regarding their flow cytometry experience. With our aggregated database of numerous reagent vendors' catalogs, we will create a database of designed panels from published sources to facilitate efficient reuse of panels and promote data sharing. We will base our work on nascent standards being created in the field, specifically MIFlowCyt, OMIP, ACS, and NetCDF, as well as innovation in the creation of a reagent ontology for cytometry. We will also define requirements for the development of an expert system approach to panel design, that can include metrics from technical aspects of antigen density, fluorophore brightness, spectral overlap, and spillover spreading, as well as, the social computing aspects of factoring in specific antibody conjugate use cases with titration data. {All of these components can be accessed in the cloud online, providing a dynamic, 'living' environment for the flow cytometry community.}