Cytometry Reagent Database and Automated Panel Design Software.

细胞计数试剂数据库和自动面板设计软件。

• 批准号: 8646039
• 负责人: Isaiah Hankel
• 金额: $ 18.59万
• 依托单位: FLUORISH, LLC
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-05-01 至 2015-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8646039
• 关键词: Accounting; Achievement; Address; Algorithm Design; Algorithms; Antibodies; Antigens; Applications Grants; Area; Basic Science; Behavior; Biological Assay; Cataloging; Catalogs; Cells; Clinical Immunology; Clinical Research; Communities; Complex; Computer software; Cytometry; Data; Databases; Dependency; Development; Environment; Evaluation; Experimental Designs; Expert Systems; Flow Cytometry; Fluorochrome; Goals; Growth; Immune Targeting; Immune system; Immunophenotyping; Indium; Knowledge; Laboratories; Life; Link; Marketing; Methods; Metric; Modeling; Monitor; Online Systems; Ontology; Phenotype; Positioning Attribute; Process; Published Comment; Publishing; Quality Control; Reagent; Research; Research Infrastructure; Research Personnel; Resources; Scientist; Site; Social Network; Software Design; Solutions; Source; Staining method; Stains; System; Technology; Testing; Time; Titrations; Training Support; Vendor; Work; antibody conjugate; base; cloud based; commercial application; data sharing; database design; density; design; e-commerce; experience; experimental analysis; fluorophore; heuristics; indexing; innovation; instrument; interest; novel; novel vaccines; public health relevance; real world application; social; success; tool; vaccine development; wasting

DESCRIPTION (provided by applicant): The proposed project described in this grant application, combined with existing Fluorish tools, will create a robust cloud-based e-commerce marketplace for cytometry research reagents, addressing key needs in the research community. Flow Cytometry is widely used in basic and clinical research. Fluorish LLC will streamline the experimental design process and enhance the quality of scientific inquiry for flow cytometry research. Through iterative development, we will begin implementing an automated panel design system, and a comprehensive reagent and panel database. Linking these components in the cloud with Fluorish's social computing capabilities, will help users pursue greater complexity in multiparametric flow cytometry experimental design and significantly enhance their scientific exploration. Objectives: {(1) Provide an online cloud environment that allows researchers to develop valuable social networks, while integrating robust scientific experimental design tools, with core/lab management systems, and an e-commerce online marketplace.} (2) Develop and implement an experimental design schema, with integrated evaluation metrics, that will automatically characterize the interaction between reagent and instrument dependencies, to account for the complexities of fluorophore assignments in panel design. (3) Create heuristics with a common ontology for flow cytometry reagents and panels that will allow for greater transparency on use of reagents, and sharing capabilities of optimized flow cytometry panels. Methods: Building on our existing core and lab management systems, we will continue to add functionality to our site, allowing scientists to readily manage and distribute critical informatio regarding their flow cytometry experience. With our aggregated database of numerous reagent vendors' catalogs, we will create a database of designed panels from published sources to facilitate efficient reuse of panels and promote data sharing. We will base our work on nascent standards being created in the field, specifically MIFlowCyt, OMIP, ACS, and NetCDF, as well as innovation in the creation of a reagent ontology for cytometry. We will also define requirements for the development of an expert system approach to panel design, that can include metrics from technical aspects of antigen density, fluorophore brightness, spectral overlap, and spillover spreading, as well as, the social computing aspects of factoring in specific antibody conjugate use cases with titration data. {All of these components can be accessed in the cloud online, providing a dynamic, 'living' environment for the flow cytometry community.}

描述（由申请人提供）：本资助申请中描述的拟议项目与现有的Fluorish工具相结合，将为细胞计数研究试剂创建一个强大的基于云的电子商务市场，以满足研究社区的关键需求。流式细胞术广泛应用于基础和临床研究。Fluorish LLC将简化实验设计过程，提高流式细胞术研究的科学探究质量。通过迭代开发，我们将开始实施自动化试剂盒设计系统以及全面的试剂和试剂盒数据库。将云中的这些组件与Fluorish的社交计算能力相连接，将有助于用户在多参数流式细胞术实验设计中追求更高的复杂性，并显着增强他们的科学探索。 目的：{（1）提供在线云环境，使研究人员能够开发有价值的社交网络，同时将强大的科学实验设计工具与核心/实验室管理系统和电子商务在线市场集成。} (2)开发和实施一个实验设计方案，与综合评价指标，将自动表征试剂和仪器依赖性之间的相互作用，以解释面板设计中荧光团分配的复杂性。(3)为流式细胞仪试剂和样本组创建具有通用本体的分析学，从而提高试剂使用的透明度，并共享优化流式细胞仪样本组的功能。 研究方法：在我们现有的核心和实验室管理系统的基础上，我们将继续为我们的网站添加功能，使科学家能够随时管理和分发有关其流式细胞术经验的关键信息。我们将通过汇集众多试剂供应商目录的数据库，从已发布的来源创建设计样本组的数据库，以促进样本组的高效重复使用并促进数据共享。我们的工作将基于该领域正在创建的新生标准，特别是MIFlowCyt，OMIP，ACS和NetCDF，以及创建细胞计数试剂本体的创新。我们还将定义专家系统方法开发的要求，以面板设计，可以包括从抗原密度，荧光团亮度，光谱重叠和溢出扩散的技术方面的指标，以及，在具体的因素的社会计算方面。 抗体偶联物使用案例和滴定数据。{All这些组件中的一部分可以在云中在线访问，为流式细胞术社区提供动态的“生活”环境。