Cytometry Reagent Database and Automated Panel Design Software.

描述(由申请人提供)：本拨款申请中描述的拟议项目，与现有的氟工具相结合，将为细胞学研究试剂创建一个强大的基于云的电子商务市场，满足研究界的关键需求。流式细胞术广泛应用于基础和临床研究。FLORISH LLC将简化实验设计流程，提高流式细胞仪研究的科学探究质量。通过迭代开发，我们将开始实施自动化面板设计系统，以及一个全面的试剂和面板数据库。将云中的这些组件与Fluish的社会计算能力相结合，将帮助用户在多参数流式细胞仪实验设计中追求更大的复杂性，并显著增强他们的科学探索能力。 目标：{(1)提供一个在线云环境，允许研究人员开发有价值的社交网络，同时将强大的科学实验设计工具与核心/实验室管理系统和电子商务在线市场相集成。}(2)开发和实施一个实验设计方案，带有集成的评估指标，将自动表征试剂和仪器依赖之间的相互作用，以考虑面板设计中荧光团分配的复杂性。(3)为流式细胞仪试剂和仪表板创建具有通用本体的启发式算法，以提高试剂使用的透明度，并共享优化的流式细胞仪仪表板的功能。 方法：在我们现有的核心和实验室管理系统的基础上，我们将继续为我们的网站增加功能，使科学家能够轻松地管理和分发有关他们的流式细胞仪经验的关键信息。通过我们汇集了众多试剂供应商目录的数据库，我们将根据公布的来源创建一个设计面板的数据库，以促进面板的高效重复使用和促进数据共享。我们的工作将基于该领域正在创建的新标准，特别是MIFlowCyt、OMIP、ACS和NetCDF，以及在创建用于细胞学的试剂本体方面的创新。我们还将定义开发面板设计的专家系统方法的要求，其中可以包括来自抗原密度、荧光团亮度、光谱重叠和溢出扩散等技术方面的指标，以及特定因素的社会计算方面的指标。 抗体结合使用案例和滴定数据。{所有这些组件都可以在云中在线访问，为流式细胞仪社区提供了一个动态的“生活”环境。}

DESCRIPTION (provided by applicant): The proposed project described in this grant application, combined with existing Fluorish tools, will create a robust cloud-based e-commerce marketplace for cytometry research reagents, addressing key needs in the research community. Flow Cytometry is widely used in basic and clinical research. Fluorish LLC will streamline the experimental design process and enhance the quality of scientific inquiry for flow cytometry research. Through iterative development, we will begin implementing an automated panel design system, and a comprehensive reagent and panel database. Linking these components in the cloud with Fluorish's social computing capabilities, will help users pursue greater complexity in multiparametric flow cytometry experimental design and significantly enhance their scientific exploration. Objectives: {(1) Provide an online cloud environment that allows researchers to develop valuable social networks, while integrating robust scientific experimental design tools, with core/lab management systems, and an e-commerce online marketplace.} (2) Develop and implement an experimental design schema, with integrated evaluation metrics, that will automatically characterize the interaction between reagent and instrument dependencies, to account for the complexities of fluorophore assignments in panel design. (3) Create heuristics with a common ontology for flow cytometry reagents and panels that will allow for greater transparency on use of reagents, and sharing capabilities of optimized flow cytometry panels. Methods: Building on our existing core and lab management systems, we will continue to add functionality to our site, allowing scientists to readily manage and distribute critical informatio regarding their flow cytometry experience. With our aggregated database of numerous reagent vendors' catalogs, we will create a database of designed panels from published sources to facilitate efficient reuse of panels and promote data sharing. We will base our work on nascent standards being created in the field, specifically MIFlowCyt, OMIP, ACS, and NetCDF, as well as innovation in the creation of a reagent ontology for cytometry. We will also define requirements for the development of an expert system approach to panel design, that can include metrics from technical aspects of antigen density, fluorophore brightness, spectral overlap, and spillover spreading, as well as, the social computing aspects of factoring in specific antibody conjugate use cases with titration data. {All of these components can be accessed in the cloud online, providing a dynamic, 'living' environment for the flow cytometry community.}