ELECTROPHYSIOLOGY - TUSM/T-NEMC CENTER FOR NEUROSCIENCE RESEARCH

电生理学数据通常被认为是证明细胞/电路生理学和行为之间因果关系的金标准。然而，对于一个缺乏多年培训的研究者来说，产生初步数据是一个令人生畏的前景，而这些培训是培养一个称职的独立电生理学家所必需的。电生理学研究还需要在人员和设备方面进行相当大的前期投资，这进一步阻碍了研究人员进入电生理学竞技场。当EBC首次成立时，塔夫茨大学的电生理学社区很小（考克斯和邓拉普是该学院唯一的电生理学家），但仍有大量的教师寻求将电生理学方法应用于他们的研究项目。因此，EBC的建立是为了促进电生理学在塔夫茨神经科学研究实验室中的更广泛应用。这一使命的实现比我们想象的要彻底得多（详见C5.2.3i）。 自2003年CNR成立以来，塔夫茨的电生理学社区发生了巨大的变化， 部分原因是EBC的成功。几位研究者在《电生理学入门》中接受了介绍。 EBC。值得注意的是，其中四个（Feig，Galper，Jacob，Rios）现在在他们的实验室里有学生和/或研究员， 独立地在他们自己的实验室或EBC中的装置上进行这样的实验。此外，最近神经科学部门的扩张进一步改变了CNR用户的人口统计数据。电生理记录现在是考克斯、邓拉普、杜拉、菲格、加尔珀、海顿、雅各布、马奎尔、莫斯、里奥斯和特里默实验室的关键。随着电生理学社区的增长，（i）需要访问EBC现有设置的用户数量正在接近稳定状态，（ii）大多数EBC用户经过充分培训，不仅可以独立进行实验，还可以帮助培训未来的实验室人员，以及（iii）随着程序采用更多的行为焦点，对体内刺激/记录的需求越来越大。这种演变正在推动EBC的变化（如下所述），使我们能够继续满足塔夫茨神经科学界的电生理需求。

Electrophysiological data are often considered the gold standard for demonstrating a causal relationship between cell/circuit physiology and behavior. Generating preliminary data is a daunting prospect, however, for an investigator who lacks the years of training necessary to produce a competent, independent electrophysiologist. Electrophysiological studies also require a considerable up-front investment in personnel and equipment, further discouraging investigators from entering the electrophysiological arena. When the EBC was first established, the electrophysiology community at Tufts was small (Cox and Dunlap being the only electrophysiologists on the faculty), yet there were significant numbers of faculty seeking to bring electrophysiological approaches to bear on their research projects. The EBC was, thus, established to promote more widespread use of electrophysiology in neuroscience research laboratories at Tufts. This mission has been realized more completely than we thought possible (see C5.2.3i for details). Tufts' electrophysiology community has changed dramatically since the CNR was established in 2003, in part due to the success of the EBC. Several investigators received their introduction to electrophysiology in the EBC. Remarkably, four of these (Feig, Galper, Jacob, Rios) now have students and/or fellows in their labs who independently perform such experiments on setups in their own labs or in the EBC. In addition, the recent Neuroscience Department expansion has further changed the demographics of CNR users. Electrophysiological recording is now key in the labs of Cox, Dunlap, Dulla, Feig, Galper, Haydon, Jacob, Maguire, Moss, Rios, and Trimmer. With this growth of the electrophysiology community, (i) the number of users needing access to EBC's existing setups is approaching a steady-state, (ii) the majority of EBC users are sufficiently trained to not only carry out their experiments independently, but to help train future lab personnel, and (iii) there is increasing demand for in vivo stimulation/recording as programs adopt a more behavioral focus. This evolution is driving changes in the EBC (described below) to allow us to continue to meet the electrophysiological needs of the Tufts neuroscience community.