Supplement to the TUSM/T-NEMC Center Core for Neuroscience Research

这是一项补充申请，申请资金以增加现有的仪器设备和人员 NINDS资助的中心核心(塔夫茨神经科学研究中心，CNR)。几个排位赛中锋 新英格兰塔夫茨大学医学院的成员和其他NINDS资助的调查人员 医疗中心(Tuft-NEMC)需要在表达谱中使用高密度Affymetrix基因阵列 学习。尽管在Tuft(Tuft-NEMC表达式数组核心，TEAC)中存在表达式数组核心，但这 设施只包括产生和分析cDNA或寡核苷酸基因阵列的设备；它不包括 为Affymetrix芯片杂交、清洗和扫描提供仪器。事实上，没有一个 塔夫茨大学或塔夫斯-NEMC任意系内的Affymetrix系统。我们提供初步数据和 补充资金购买完整Affymetrix系统的理由。新的Affymetrix系统将 位于现有的TEAC设施内；它将与现有的生物信息学核心很好地吻合， 目前由我们的中心拨款资助。我们的生物信息学核心目前提供微阵列数据分析 为中心成员和其他进行基因图谱研究的调查人员提供服务。除了……之外 工具，此应用程序请求为技术人员提供工资支持以维护新的Affymetrix 系统，并为NINDS和其他调查人员进行实验。技术人员将由以下人员监督 Dale Hunter博士，同时担任生物信息学和TEAC核心设施的主任 作为TEAC工厂的经理兰卫女士。塔夫茨大学引进Affymetrix系统 将补充现有的表达阵列设施，并为研究计划提供显著的附加值 NINDS资助的研究人员和塔夫茨大学和塔夫茨-NEMC的其他科学家。

This is a supplemental application requesting funds to add instrumentation and personnel to an existing NINDS-funded center core (the Tufts Center for Neuroscience Research, CNR). Several qualifying center members and other NINDS-funded investigators at Tufts University School of Medicine and Tufts-New England Medical Center (Tufts-NEMC) have a need to employ high-density Affymetrix gene arrays in expression profiling studies. Although an expression array core exists at Tufts (the Tufts-NEMC Expression Array Core, TEAC), this facility only includes equipment for generating and analyzing cDNA or oligonucleotide gene arrays; it does not provide instrumentation for Affymetrix chip hybridization, washing and scanning. Indeed, there is not a single Affymetrix system within any department of Tufts University or Tufts-NEMC. We provide preliminary data and justifications for supplemental funds to purchase a complete Affymetrix system. The new Affymetrix system will be located within the existing TEAC facility; it will dovetail well with an existing bioinformatics core that is currently funded by our center grant. Our bioinformatics core currently provides microarray data analysis services to center members and other investigators who perform gene-profiling studies. In addition to instrumentation, this application requests salary support for a technician to maintain the new Affymetrix system and conduct experiments for NINDS and other investigators. The technician will be supervised by Dr. Dale Hunter, who serves as director of both the bioinformatics and the TEAC core facilities, as well as Ms. Lan Wei, the manager of the TEAC facility. The introduction of an Affymetrix system at Tufts University will complement existing expression array facilities and provide significant added value to the research programs of NINDS-funded investigators and other scientists at Tufts University and Tufts-NEMC.