Animal and Microsurgery Core

动物和显微外科核心

• 批准号: 8512664
• 负责人: Maria-Luisa Alegre
• 金额: $ 29.58万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-01 至 2017-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8512664
• 关键词: Anesthetics; Animals; Breeding; Cells; Ensure; Genotype; Health; Heart Transplantation; Histology; Human Resources; Immune; Immunosuppressive Agents; Infection; Maintenance; Microsurgery; Monitor; Mus; Operative Surgical Procedures; Organ; Principal Investigator; Procedures; Protocols documentation; Reagent; Research Personnel; Resources; Role; Services; Source; Spleen; Splenocyte; Techniques; Therapeutic immunosuppression; Thymectomy; Transplant Recipients; Transplantation; Transplantation Tolerance; allograft rejection; animal breeding; cost; heart allograft; lymph nodes; programs; research study; screening

The purpose of the Animal and Microsurgery Core (Core B) is to establish a focused facility that provides investigators in each of the two projects with sufficient breeding of animals with given genotypes and with a standardized cardiac transplantation and tolerance induction procedure for the proposed experiments. Core B will provide a key service role that will enable the Principal Invesfigators and their staff to focus on experimental and scientific efforts. Centralized breeding and screening of the progeny will ensure that animals of required genotypes are available for experiments. Transplantafion performed by a centralized source will standardize the procedures, minimize surgically imposed disparities in the grafts used by the two projects and allow some long-term acceptors generated for Project 1 (studying the induction of tolerance) to be used for Project 2 (studying the maintenance of tolerance) to minimize experimental cost. In addifion, purchases of reagents such as anesthetics and immunosuppressants will be done in bulk for both projects to reduce cost. The specific tasks of Core B will be to: a) Ijreed and genotype animals to be used for both projects; b) transplant heart allografts and perform thymectomies as directecj by the project leaders and their personnel; c) prepare and inject donor splenocytes and administer indicated immunosuppressive therapies; d) monitor the beafing of the transplanted hearts, the health of the transplant recipients, and record fimes of rejecfion; e) sacrifice transplanted animals as directed by the Principal Investigators and their personnel and collect organs for histology, as well as to distribute to other project personnel for phenotypic and functional analysis of immune cells.

动物和显微外科核心（核心 B）的目的是建立一个重点设施，提供 这两个项目的研究人员都充分培育了具有给定基因型和 所提出的实验的标准化心脏移植和耐受诱导程序。核心B 将提供关键的服务角色，使首席研究员及其员工能够专注于 实验和科学努力。集中繁育和筛选后代将确保 所需基因型的动物可用于实验。移植由集中进行 来源将标准化程序，最大限度地减少手术造成的两个移植物的差异 项目并允许为项目 1（研究耐受性的归纳）生成的一些长期接受者 用于项目 2（研究耐受性的维持）以最小化实验成本。另外， 这两个项目将批量采购麻醉剂和免疫抑制剂等试剂 降低成本。核心 B 的具体任务是： a) Ijreed 和基因分型动物用于两者 项目； b) 在项目负责人和他们的指导下进行同种异体心脏移植和胸腺切除术 人员; c) 准备并注射供体脾细胞并进行指定的免疫抑制治疗； d) 监测移植心脏的搏动情况、移植受者的健康状况，并记录移植时间 拒绝； e) 按照首席研究员及其人员的指示处死移植动物，以及 收集器官进行组织学分析，并分发给其他项目人员进行表型和功能分析 免疫细胞分析。