Meniscus Regeneration by Endogenous Stem/Progenitor Cells

内源干/祖细胞的半月板再生

• 批准号: 8697945
• 负责人: LISA A FORTIER
• 金额: $ 101.24万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-05-01 至 2019-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8697945
• 关键词: Adult; Allografting; American; Animal Model; Arthritis; Biomedical Engineering; Bone Marrow; Cadaver; Cell Culture Techniques; Cell Differentiation process; Cell Separation; Cell Transplantation; Cells; Clinical; Collagen; Collagen Type I; Collagen Type II; Cues; Data; Defect; Degenerative polyarthritis; Derivation procedure; Distal; Femur; Fibrocartilages; Flow Cytometry; Goals; Growth Factor; Healed; Histology; Human; Immunohistochemistry; In Vitro; Incidence; Incubated; Injury; Intervertebral disc structure; Joints; Knee; Knee joint; Knowledge; Leg Bones; Life; Magnetic Resonance; Mechanics; Medicine; Meniscus structure of joint; Mesenchymal; Mesenchymal Stem Cells; Natural regeneration; Orthopedics; Pain; Patients; Phenotype; Play; Population; Pre-Clinical Model; Property; Proteins; Recombinants; Recruitment Activity; Research Project Grants; Resected; Resort; Rheumatoid Arthritis; Risk; Role; Sampling; Shapes; Sheep; Shock; Source; Stem cells; Stress; Structure; Surface; Synovial Membrane; Temporomandibular Joint; Tendon structure; Testing; Thigh structure; Time; Tissues; Transforming Growth Factors; Transplantation; Trauma; United States; absorption; bone; computerized; connective tissue growth factor; efficacy testing; healing; in vivo; meetings; pathogen; public health relevance; regenerative therapy; response; scaffold; spatiotemporal; stem; tibia; transmission process

DESCRIPTION (provided by applicant): The meniscus is a semi-lunar and wedge-shaped fibrocartilaginous structure between the distal femoral condyle and the proximal tibial plateaus in the knee joint. Meniscus plays indispensable roles in joint congruence, shock absorption, and stress transmission. Meniscus injuries do not heal spontaneously. Clinically, over one million patients undergo meniscectomy each year in the United States. Allograft transplantation from cadavers is the primary clinical substitute of resected meniscus, but suffers from donor shortage, pathogen transmission, immunorejection and tissue mis-match. Meniscectomy, with or without allograft transplantation, alleviates pain at best, but significantly increases the incidence of osteoarthritis later in life. By 2020, a total of 67 million Americans will suffer fro arthritis. Not surprisingly, regeneration of knee meniscus is an aspiring goal in orthopedic medicine, but has encountered multiple barriers. No regenerative therapies exist for meniscus injuries at this time. One of the critical barriers of meniscus regeneration is our insufficient knowledge of meniscus cells, known as fibrochondrocytes whose origin and lineage derivation are poorly understood. Our preliminary data demonstrate that 1) fibrochondrocytes were derived from adult stem/progenitor cells by step-wise induction with two recombinant human growth factors that are both necessary and sufficient for induction of fibrochondrocytes in vitro and in vivo; 2) spatiotemporal release of these two growth factors induced de novo formation of multiphase fibrocartilage tissues in anatomically correct bioscaffolds that replaced partially resected knee meniscus in vivo in a preclinical model. Host endogenous cells were recruited into anatomically correct, microporous scaffolds and produced primarily type I collagen in the outer region, type II collagen in the inner region and blended type I and II collagens in the intermediate zone of the regenerated meniscus tissues. The overall objectives of this proposal are to optimize strategies for the recruitment of host endogenous cells and differentiation into fibrochondrocytes in our existing pre-clinical model of meniscus regeneration, without cell transplantation. Our overarching hypothesis is that spatiotemporal delivery of specific bioactive cues regulates the recruitment and fibrochondrogenic differentiation of endogenous cells, including stem/progenitor cells, towards meniscus regeneration. No regenerative therapies exist for fibrocartilage defects in intervertebral disks, tendon-bone junction and the temporomandibular joint. The planned studies will identify fibrochondrocyte populations that are pivotal for meniscus regeneration, and may have implications for the regeneration of other fibrocartilage structures. The planned cell recruitment strategies may be applicable in the regeneration of other tissues.

描述（申请人提供）：半月板是位于股骨远端髁和胫骨近端平台之间的半月形楔形纤维软骨结构