Recombinant Virus and Vector Core

重组病毒和载体核心

• 批准号: 8541141
• 负责人: YAN YUAN
• 金额: $ 27万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-05-08 至 2018-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8541141
• 关键词: Arts; B-Lymphocytes; Cells; Complementary DNA; Consultations; Databases; Engineering; Event; Generations; Genetic Recombination; Goals; Grant; Herpesviridae; Human Herpesvirus 8; Infection; Instruction; Laboratories; Lentivirus; Methods; Molecular; Mutagenesis; Mutation; Online Systems; Personal Communication; Production; Reagent; Recombinants; Resources; Role; Services; Site; Suggestion; Viral Genome; Viral Proteins; Viral Vector; Virion; Virus; base; design; expression vector; large scale production; mutant; particle; programs; recombinant virus; repository; response; small hairpin RNA; vector

SUMMARY (See instructions): The Recombineering and Virus Production core will be responsible for 1) recombinant virus design consultation, 2) vector and viral reagent repository; 3) generation of site-directed mutations in Bacmid KSHV, and 4) large scale production of recombinant and wild-type KSHV. These goals will be accomplished by a centralized laboratory, providing a set of standardized services, including state ofthe art methods in herpes virus, molecular engineering and recombination-based mutagenesis (i.e. Kan-SacB, Kan-rpsL, Gal-K, and LoxP-kan for conditional knock-outs), production of recombinant Lenti-virus expression vectors for cDNA and shRNA, and production of KSHV from transfected 293 cells (recombinant viruses), GFP tagged viruses with specific, mutations, or BCBL1 cells (wild-type virus). Since it is expected that this program project will generate unique resources for research, the core will be responsible for storage of recombinant viruses, bacmids, reagents for recombineering and Lenti-virus production, as well as management of adequate databases that users can access and search via a web-based browser. Expertise in all aspects of protein and virus production will be provided to the projects for the lifetime of grant support.

总结（参见说明）：工程和病毒生产核心将负责1）重组病毒设计咨询，2）载体和病毒试剂库; 3）在Bacmid KSHV中产生定点突变，以及4）重组和野生型KSHV的大规模生产。这些目标将由一个中央实验室完成，提供一系列标准化服务，包括疱疹病毒、分子工程和基于重组的诱变的最新方法（即Kan-SacB、Kan-rpsL、Gal-K和LoxP-kan用于条件性敲除），用于cDNA和shRNA的重组慢病毒表达载体的产生，以及从转染的293细胞（重组病毒）、具有特异性突变的GFP标记的病毒或BCBL 1细胞（野生型病毒）产生KSHV。由于预计该计划项目将产生独特的研究资源，核心将负责重组病毒，细菌，重组工程和慢病毒生产试剂的存储，以及用户可以通过基于Web的浏览器访问和搜索的适当数据库的管理。在赠款支持的生命周期内，将向这些项目提供蛋白质和病毒生产各方面的专门知识。