Immunohistochemical analysis of tumors

为了获得可能对研究肿瘤类型中特定抗原的存在有用的标本，接受了匿名组织捐赠。我们描述了一种在没有特定设备的情况下生成多肿瘤块的方法。在这些组织中，我们研究了几种内皮抗原的分布：claudin 5、PROX1和VEGFR2(KDR基因产物)。同时检测KBA62和PNL-2抗原在黑色素瘤和其他肿瘤中的分布。我们发现VEGFR2在间叶性癌中对内皮细胞肿瘤具有高度特异性，但恶性间皮瘤是唯一的VEGFR2强阳性的上皮性癌。我们还与NCI的儿科GIST诊所合作，研究了琥珀酸脱氢酶缺乏的胃肠道间质瘤。

As an effort to obtain specimens that might be useful in the study of the presence on specific antigens in tumor types, anonymous tissue donations were accepted. We described a method to generate multitumor blocks without specific equipment. With these tissues, we characterized the distribution of several endothelial antigens: Claudin 5, PROX1, and VEGFR2 (KDR gene product). Also distribution of KBA62 and PNL 2 antigens in melanoma and other tumors was examined. We found VEGFR2 highly specific for endothelial tumors among mesenchymal cancers, but malignant mesotheliomas were found to be unique epithelial cancers strongly positive for VEGFR2. We also examined succinate dehydrogenase deficient gastrointestinal stromal tumors, in collaboration with NCI's pediatric GIST clinic.