Tissue Engineering of New Hair Formation

描述（由申请人提供）：本提案的目标是开发可从游离干细胞形成新毛发的重建皮肤。利用分离的多电位真皮和表皮干细胞制造毛囊的研究取得了进展。然而，在临床应用取得重大进展之前，还需要填补一些空白。最近，我们大大改进了Lichti和Stenn之前的检测方法，使其成为一种更简单的毛发形成检测方法，具有临床正确的外观和高通量检测的潜力。我们的一般假设是，多能性皮肤干细胞可以通过分子重编程产生，毛发原基的排列和模式可以受环境条件的调节。利用新开发的“平面毛发形成程序”，我们能够运用我们在羽毛图案形成方面的专业知识来调节毛发原基的数量和大小。我们还将运用我们最近在毛干细胞宏观环境调控方面的经验来提高毛发形成的效率。最近在其他器官中的分子重编程取得了成功，这激发了人们的兴奋。这种简单的分析将允许我们进行高通量分析，以鉴定对成人皮肤细胞重编程为形成毛发的真皮和表皮细胞重要的分子或分子组合。这项技术的发展将给那些遭受严重创伤和脱发的人带来新的希望。

DESCRIPTION (provided by applicant): The objective of this proposal is to develop reconstituted skin that can form new hairs from dissociated stem cells. Progress has been made in producing hair follicles using dissociated multi-potential dermal and epidermal stem cells. However, couple gaps need to be filled before significant progress in clinical applications can be made. Recently, we have improved significantly the previous assays by Lichti and Stenn into a simpler hair forming assay with clinically proper appearance and potential for a high throughput assay. Our general hypothesis is that multi-potent skin stem cells can be generated by molecular reprogramming, and that arrangement and patterning of hair primordia can be regulated by environmental conditions. With the newly developed "planar hair forming procedure", we are able to apply our expertise in feather pattern formation to regulate the number and size of hair primordia. We will also apply our recent experience on macro-environment regulation of hair stem cells to improve the efficiency of hair formation. Recent success of molecular re-programmig in other organs has inspired excitement. This simple assay will allow us to do high throughput analyses to identify molecules or combinations of molecules important to reprogram adult skin cells into hair forming dermal and epidermal cells. The development of this technology will bring new hope to those who suffer from severe wounds and alopecia.