Live Cell Imaging and Protein Analytical Core

在准备即将到来的计划项目拨款（POl）更新的背景下，在过去的五年里，我们开发了新颖的尖端成像方法，建立了与0x43分析相关的先进蛋白质分析方法，并进一步培训了越来越多的项目人员进行一般Ca2+成像和0x43蛋白质分析。我们在完整血管内皮细胞的双Ca2+/N0检测方面也发展了相当多的专业知识，所有这些方法现在都日益成为该联合POl项目的核心。在目前的申请中，将通过这个新的核心B继续对现有人员和新人员进行持续培训，并对成像方法应用进行持续的技术支持，并将为成像显微镜增加持续的仪器技术支持。此外，Core将为基于Western blot和Co-IP的细胞蛋白分析提供培训支持

In the context of preparing for this upcoming Program Project Grant (POl) renewal, over the last five years we have developed novel and cutting edge imaging methodology and established advanced protein analytical methods related to 0x43 analysis, as well as further training an expanding group of project personnel in general Ca2+ imaging and 0x43 protein analysis. We have also developed considerable expertise in dual Ca2+/N0 detection in endothelial cells of intact vessels and all these methods are now increasingly central to the projects of this combined POl. In the current application, continued training of current and new personnel and ongoing technical support of imaging methods application will continue through this new Core B and ongoing instrument technical support will be added for imaging microscopy. In addition, the Core will provide training support for cell protein analysis by Western blot based and Co-IP procedures as well as new antibody and technical assay development for all four projects. As a coordinating unit, the Core will offer further support to enhance data collection uniformity by relieving project personnel of the burden of preparing frozen stocks of validated pooled endothelial cells for imaging, so ensuring both high qualities of cell preparations providing uniformity of data acquisition between the projects. Where opportunities for reagent purchase can improve cost savings, or at least ensure more abundant reagents are fully used, the Core will coordinate those supplies to secure savings.