G-CSF prevents male infertility after chemotherapy.

项目摘要。精原干细胞是哺乳动物睾丸中的成体干细胞， 精子发生和对男性生育力至关重要。癌症后SSC丢失导致的男性不育症 治疗（例如，化疗和放疗）是许多癌症幸存者的生活质量问题。在这 根据这项提议，我们将测试一种新的方法，在癌症治疗后保留男性生育能力， 注射细胞因子粒细胞集落刺激因子（G-CSF）。在目标1中，我们将证实G-CSF 给药预防化疗后精子发生和生育能力的丧失，并确定最佳的 治疗方案，以最大限度地发挥这些有益的效果。在目标2中，我们将确定G-CSF是否 治疗在SSC水平起作用，并将开始解决其保护作用机制。如果发现 有效的G-CSF治疗将完全消除对有风险的侵入性干预的需要，因此， 显著改善癌症治疗后保留男性生育能力的临床范例的潜力。

Project Summary. Spermatogonial stem cells (SSCs) are adult stem cells in mammalian testes that maintain spermatogenesis and are essential for male fertility. Male infertility resulting from SSC loss after cancer treatments (e.g., chemotherapy and radiation) is a quality-of-life issue for many cancer survivors. In this proposal, we will test a novel approach to preserving male fertility after cancer treatment that employs injections of the cytokine granulocyte colony-stimulating factor (G-CSF). In Aim 1, we will confirm that G-CSF administration prevents loss of spermatogenesis and fertility after chemotherapy and determine the optimal treatment regimen to maximize these beneficial effects. In Aim 2, we will determine whether G-CSF treatments act at the level of SSCs and will begin to address its protective mechanism of action. If found to be effective, G-CSF treatment would completely obviate the need for risky invasive interventions, and thus, has the potential to significantly improve the clinical paradigm for preserving male fertility after cancer treatment.