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Coherent light scattering for early detection of retinal disease

相干光散射用于早期检测视网膜疾病

基本信息

批准号：
8626404
负责人：
Adam Wax
金额：
$ 18.96万
依托单位：
DUKE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-03-01 至 2016-02-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8626404
关键词：
Animal Model Animals Barrett Esophagus Biological Markers Cell Nucleus Cells Cellular Structures Characteristics Clinical Cone Data Detection Development Devices Diagnosis Diagnostic Disease Disease Progression Dysplasia in Barrett&apos s Esophagus Early Diagnosis Epithelial Cells Epithelium Esophageal Eye Future Glaucoma Goals Health Human Image Imaging Techniques Imaging technology Individual Interferometry Knock-out Life Measurement Measures Methods Metric Modality Modeling Morphology Mus Nerve Degeneration Normal tissue morphology Nuclear Ocular Pathology Ophthalmoscopes Optical Coherence Tomography Optics Organizational Change Pathology Patients Pharmaceutical Preparations Photoreceptors Premalignant Cell Public Health Research Resolution Retina Retinal Retinal Degeneration Retinal Diseases Retinitis Pigmentosa Rhodopsin Scanning Staging Structure System Techniques Technology Thick Time Tissues Treatment Efficacy Visual system structure adaptive optics base clinical application density design improved in vivo light scattering meetings non-invasive imaging novel optical imaging portability prototype public health relevance research study retinal nerve fiber layer retinal neuron retinal rods screening

项目摘要

DESCRIPTION (provided by applicant): The overall objective of this proposal is to develop a novel in vivo optical imaging system that will quantify structural biomarkers of retinal degenerative diseases. The state-of-the-art technology is measurement of the thickness of retina layers by spectral domain optical coherence tomography (SDOCT). However, while SDOCT can resolve histological layers, it lacks the resolution to measure cellular features. In recent SDOCT studies, only a modest correlation between the thickness of the retinal nerve fiber layer and the progression of glaucoma has been shown, with similar findings seen between the thickness of the photoreceptor layer and progression of retinitis pigmentosa. This reflects the fact that thickness of an individual retina layer does not necessarily correlate with the healt or anatomical condition of its constituent cells. We have shown that angle-resolved low coherence interferometry (a/LCI), a light scattering method that incorporates the depth resolution of OCT, can acquire depth-resolved morphological measurements of esophageal epithelial cells, which allows in vivo early detection of dysplasia in Barrett's Esophagus patients Unlike other non-invasive imaging techniques, a/LCI allows direct and specific measurements of the nuclear morphology of epithelial cells within intact, unstained tissues. We have shown that a/LCI can detect changes in organization of cells in the retinal layer due to pathology. We now propose to apply a/LCI to the diagnosis of ocular diseases by developing an a/LCI imaging system that is compatible with a scanning ophthalmoscope in order to execute depth-resolved measurements of the nuclear morphology of retinal neurons. Here we will seek to formulate accurate quantitative biomarkers that predict the onset and progression of neurodegenerative ocular pathologies based on these structural and organizational changes. In this project, (1) we will implement a prototype a/LCI system to enable in vivo studies of the retina and (2) apply the system to a detailed time course study of a murine model of progressive retinal degeneration to characterize the changes in both size and organization of photoreceptor structures as a function of disease progression. Upon successful completion of this exploratory research study, there will be justification to pursue development of a clinical a/LCI device for application to humans.

描述（由申请人提供）：本提案的总体目标是开发一种新型体内光学成像系统，该系统将量化视网膜退行性疾病的结构生物标志物。最先进的技术是通过光谱域光学相干断层扫描（SDOCT）测量视网膜层的厚度。然而，虽然SDOCT可以分辨组织学层，但它缺乏测量细胞特征的分辨率。在最近的SDOCT研究中，仅显示了视网膜神经纤维层厚度与青光眼进展之间的适度相关性，在感光层厚度与视网膜色素变性进展之间观察到类似的发现。这反映了单个视网膜层的厚度不一定与其组成细胞的健康或解剖学状况相关的事实。我们已经证明，角度分辨低相干干涉测量（a/LCI），一种结合OCT深度分辨率的光散射方法，可以获得食管上皮细胞的深度分辨形态测量，这使得在体内早期检测巴雷特食管患者的异型增生。与其他非侵入性成像技术不同，a/LCI允许直接和特异性测量完整、未染色组织内上皮细胞的核形态。我们已经表明，a/LCI可以检测由于病理学导致的视网膜层细胞组织的变化。我们现在建议通过开发与扫描检眼镜兼容的a/LCI成像系统来将a/LCI应用于眼部疾病的诊断，以便执行视网膜神经元的核形态的深度分辨测量。在这里，我们将寻求制定准确的定量生物标志物，预测神经退行性眼病的发病和进展的基础上，这些结构和组织的变化。在这个项目中，（1）我们将实现一个原型a/LCI系统，使视网膜的体内研究和（2）应用该系统进行性视网膜变性的小鼠模型的详细的时间过程研究，以表征作为疾病进展的函数的光感受器结构的大小和组织的变化。成功完成本探索性研究后，将有理由继续开发临床a/LCI器械用于人体。

项目成果

期刊论文数量（3）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Noise characterization of supercontinuum sources for low-coherence interferometry applications.

DOI：
10.1364/josaa.31.002703
发表时间：
2014-12-01
期刊：
Journal of the Optical Society of America. A, Optics, image science, and vision
影响因子：
0
作者：
Brown WJ;Kim S;Wax A
通讯作者：
Wax A

In vivo analysis of burns in a mouse model using spectroscopic optical coherence tomography.