Mechanisms of fibrosis exacerbation by trichloroethylene in hepatic autoimmunity

三氯乙烯加重肝脏自身免疫纤维化的机制

• 批准号: 8770165
• 负责人: James P Luyendyk
• 金额: $ 24.34万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8770165
• 关键词: Air; Applications Grants; Autoimmune Process; Autoimmune Responses; Autoimmunity; Biological Markers; Blood; Breathing; CCR1 gene; CCR5 gene; Carcinogens; Cholangitis; Collagen; Complement; Consumption; Deposition; Detection; Development; Dose; Enzymes; Epidemiologic Studies; Exposure to; Fibrosis; Gene Expression Profile; Genes; Goals; Hazardous Waste Sites; Health Hazards; Hepatic; Human; Incidence; Individual; Kidney; Leukocytes; Link; Liver; Liver Failure; Liver Fibrosis; Liver diseases; Mediating; Metabolic; Metabolism; Modeling; Molecular; Morbidity - disease rate; Mus; Non obese; Outcome Study; Pathogenesis; Pathway interactions; Patients; Predisposition; Prevalence; Primary biliary cirrhosis; Process; RANTES; RANTES receptor; RNA Sequences; Research; Research Personnel; Risk; Rodent; T-Lymphocyte; Testing; Therapeutic; Time; Tissues; Toxic Environmental Substances; Toxic effect; Trichloroethylene; United States; Urine; base; bile duct; chemokine; cytokine; diabetic; drinking water; effective therapy; environmental chemical exposure; exposed human population; gene environment interaction; human disease; impaired capacity; improved; injured; liver function; liver inflammation; liver injury; liver transplantation; meetings; mortality; mouse model; programs; public health relevance; response; superfund site; transcriptomics

DESCRIPTION (provided by applicant): Human exposure to trichloroethylene (TCE) remains a pressing concern in the United States and abroad. The categorization of TCE in recent years as a human carcinogen has further emphasized the need to completely define the potential of TCE exposure to both cause and exacerbate human disease. Strong experimental evidence from rodent studies links TCE exposure to the development of autoimmune liver disease. This connection is substantiated by epidemiological studies indicating an increased prevalence of primary biliary cirrhosis, an autoimmune liver disease, near hazardous waste sites contaminated with TCE. However, there is an insufficient understanding of the risks of TCE exposure in patients with concurrent hepatic autoimmunity, and no existing delineation of the mechanisms whereby TCE influences the progression of autoimmune liver disease. The long-term goal is to define the mechanisms whereby TCE exacerbates liver fibrosis induced by persistent autoimmune-mediated liver damage. The investigators found that exposure of mice to an occupationally-relevant TCE exposure level significantly increased liver fibrosis in mice with coexisting hepatic autoimmunity. The overall objective of these studies is to determine the effect of TCE exposure on the progression of liver fibrosis in non-obese diabetic (NOD).c3c4 mice, a mouse model of autoimmune cholangitis, and concurrently to define the impact of autoimmunity in NOD.c3c4 mice on TCE metabolism. Their central hypothesis is that TCE exposure exacerbates liver fibrosis in hepatic autoimmunity by promoting hepatic synthesis of RANTES and that TCE toxicity is further exacerbated by altered TCE metabolism in autoimmune liver disease. The investigators' grant application includes a comprehensive analysis of the dose- and time-dependent impact of TCE exposure on the pathogenesis of autoimmune liver disease in NOD.c3c4 mice. Reciprocally, these studies draw on the expertise of the investigative team to determine the effect of hepatic autoimmunity on TCE metabolism. To enable identification of mechanistic biomarkers of TCE exposure in hepatic autoimmunity, the proposed studies utilize RNA sequencing to identify genes differentially dysregulated by TCE exposure in liver and blood leukocytes. Moreover, the investigators will determine the effect of the RANTES receptor (CCR1/CCR5) antagonist Met- RANTES on the exacerbation of liver fibrosis elicited by TCE exposure in NOD.c3c4 mice. The expected outcome of these studies would be an improved understanding and detection of individual susceptibility to deleterious effects of TCE. Moreover, these studies would provide currently unavailable therapeutic strategies to counteract the exacerbation of fibrotic responses by TCE in individuals with autoimmunity.

描述（由申请人提供）：人类暴露于三氯乙烯（TCE）仍然是美国和国外的一个紧迫问题。近年来将三氯乙烯归类为人类致癌物进一步强调了需要完全确定三氯乙烯暴露导致和加剧人类疾病的可能性。来自啮齿动物研究的强有力的实验证据将TCE暴露与自身免疫性肝病的发展联系起来。流行病学研究证实了这一联系，表明在受三氯乙烯污染的危险废物场附近，原发性胆汁性肝硬化（一种自身免疫性肝病）的发病率增加。然而，目前对TCE暴露在并发肝脏自身免疫性疾病患者中的风险认识不足，也没有描述TCE影响自身免疫性肝病进展的机制。长期目标是确定TCE加重持续性自身免疫介导的肝损伤诱导的肝纤维化的机制。研究人员发现，将小鼠暴露于职业相关的TCE暴露水平显著增加了伴有肝脏自身免疫的小鼠的肝纤维化。这些研究的总体目标是确定TCE暴露对非肥胖糖尿病（NOD）.c3c4小鼠（一种自身免疫性胆管炎小鼠模型）肝纤维化进展的影响，同时确定NOD. c3 c4小鼠自身免疫对TCE代谢的影响。他们的中心假设是TCE暴露通过促进肝脏RANTES的合成而加剧了肝脏自身免疫中的肝纤维化，并且TCE毒性通过自身免疫性肝病中TCE代谢的改变而进一步加剧。研究人员的资助申请包括全面分析TCE暴露对NOD.c3c4小鼠自身免疫性肝病发病机制的剂量和时间依赖性影响。反过来，这些研究借鉴了研究团队的专业知识，以确定肝脏自身免疫对TCE代谢的影响。为了能够识别肝脏自身免疫中TCE暴露的机制生物标志物，拟议的研究利用RNA测序来识别肝脏和血液白细胞中TCE暴露差异失调的基因。此外，研究人员将确定RANTES受体（CCR 1/CCR 5）拮抗剂Met-RANTES对NOD.c3c4小鼠中TCE暴露引起的肝纤维化恶化的影响。这些研究的预期结果将是更好地理解和检测个体对三氯乙烯有害影响的易感性。此外，这些研究将提供目前不可用的治疗策略，以抵消TCE在自身免疫性个体中的纤维化反应加剧。