Role of UNC-89 (obscurin) in sarcomere assembly and maintenance.

UNC-89（obscurin）在肌节组装和维护中的作用。

• 批准号: 8632004
• 负责人: GUY Martin BENIAN
• 金额: $ 36.14万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-04-08 至 2019-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8632004
• 关键词: Adaptor Signaling Protein; Animal Model; Ankyrins; Antibodies; Binding; Biochemical; Biochemistry; Biological Process; Caenorhabditis elegans; Calcium; Calcium Signaling; Cardiomyopathies; Characteristics; Chronic Disease; Complex; Coupling; Cullin 1; Cullin Proteins; Development; Diabetes Mellitus; Elasticity; Elderly; Embryo; Enzymes; Family member; Filament; Genetic Models; Grant; Heart; Homologous Gene; Human; Immobilization; Kidney Failure; Knockout Mice; Lead; Link; Locomotion; MEL Gene; Maintenance; Malignant Neoplasms; Mediating; Medical; Microtubules; Molecular; Molecular Genetics; Mus; Muscle; Muscle Contraction; Muscular Atrophy; Muscular Dystrophies; Myofibrils; Myopathy; Myosin ATPase; Nematoda; PH Domain; Pathogenesis; Phosphotransferases; Physiological; Play; Protein Kinase; Protein phosphatase; Proteins; Regulation; Reporting; Role; SH3 Domains; Sarcomeres; Sarcoplasmic Reticulum; Scaffolding Protein; Signal Pathway; Signal Transduction; Signaling Protein; Stretching; Striated Muscles; Structure; Tertiary Protein Structure; Ubiquitin; Ubiquitination; base; connectin; copine; cullin-3; human disease; insight; katanin; loss of function; mouse model; mutant; novel; obscurin; polypeptide; protein degradation; public health relevance; scaffold; titin kinase; ubiquitin-protein ligase

Muscle sarcomeres contain a number of giant polypeptides (0.7-4 MDa). Much is currently known about the function of the largest of these polypeptides, vertebrate titin. Much less is known about the newest vertebrate member of this family, obscurin. The pathogenesis of one form of muscular dystrophy may involve obscurin. Obscurin is the homolog of UNC-89 in C. elegans. Essential features of UNC-89 as a signaling / scaffolding protein crucial for A-band/M-line assembly were discovered before obscurin was reported. To understand how UNC-89 is localized, and how it functions, we are taking a systematic approach for identifying and studying the function of its binding partners. We discovered that the Ig1-3 segment of UNC-89 interacts with CPNA-1, a copine domain protein. Although CPNA-1 is not required for initial assembly of UNC-89 at the M-line, it is required for its retention. CPNA-1 is located at both M-lines and dense bodies, whereas UNC-89 is located only at M-lines. Therefore, we hypothesize that there are proteins which direct assembly of UNC-89 solely to the M-line. UNC-89 Ig9-11 interacts with CUL-1 (cullin 1), and UNC-89 Ig2-3 interacts with MEL-26, a substrate recognition protein for CUL-3 (cullin 3). Cullins are scaffolds for assembly of the ubiquitination machinery. One function of the CUL-3/MEL-26 complex is to promote degradation of MEI-1 (katanin). Independently, the coI of this proposal, Stephan Lange, found that in mouse heart, degradation of sAnk1.5 is promoted by a cullin 3 substrate recognition protein, KCTD6, and this is dependent upon obscurin. We hypothesize that UNC-89 (obscurin) negatively regulates the activity of cullin complexes in muscle. We further hypothesize that UNC-89 interacts with other cullins or cullin 3 adaptor proteins, and there are substrates in addition to MEI-1. In humans, muscle atrophy is associated with immobilization, chronic diseases and advanced age. Since in muscle atrophy, degradation of sarcomeric proteins is upregulated, our studies have medical relevance. Also, our collaborator and coI of this grant, Ken Norman, has found that a key function of obscurin for organization of the SR, is conserved for UNC-89. In addition, UNC-89 was found to have a physiological role in EC coupling, and this involves the conserved RacGEF, VAV-1. We hypothesize that UNC-89 and VAV-1 interact, and that VAV-1 localization and function is dependent on UNC-89. Finally, we hypothesize that the binding partners and functional mechanisms discovered for UNC-89 in C. elegans are biologically relevant for mammalian striated muscle. Specific aims are: (1) determine mechanisms which direct assembly of UNC-89 solely to the M-line; (2) determine if other cullins interact with UNC-89, if there are additional substrates for MEL-26, and determine the biochemical effects of the UNC-89 to MEL-26 interaction; (3) define the biochemistry and functional consequence of interaction between UNC-89 and myosin; (4) investigate the molecular mechanism underlying UNC-89's role in calcium regulation; and (5) determine if functional interactions and molecular mechanisms identified for nematode UNC-89 are conserved for mammalian obscurin.

肌节含有大量巨多肽（0.7-4 MDa）。目前，人们对