Highly resilient, hydrophilic bioelastomers for engineering vocal fold tissue

用于工程声带组织的高弹性、亲水性生物弹性体

基本信息

  • 批准号:
    8628100
  • 负责人:
  • 金额:
    $ 47.24万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2012
  • 资助国家:
    美国
  • 起止时间:
    2012-03-20 至 2017-02-28
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Impaired voice production holds significant implications for individual health and wellness, social and occupational function, and societal productivity; the societal costs of voice problems in teachers alone have been estimated to be of the order of $2.5 billion annually in the United States. The development of materials for the treatment of vocal fold disorders, however, has been hampered by the stringent mechanical requirements of the vocal fold, which include the ability to both sustain deformation at frequencies as high as 1000Hz, and also completely recoil after transient stretch up to 200%. To date, despite widespread efforts in the development of materials scaffolds for the tissue engineering of the vocal fold, no materials with the required mechanical properties have been identified. We propose a comprehensive bioengineering approach to this problem. We will employ new elastomeric scaffolds based on the insect protein, resilin, which is the primary energy store in the sound-producing, jumping, and flight organs of insects, and demonstrates unmatched resilience (recovery after stretch) after deformation at frequencies up to 4000Hz. We will employ modular recombinant methods to generate resilin- like polypeptides (RLPs) that can be engineered to carry biologically active domains without compromising the mechanical properties of the resilin domain, and in which independent tuning of multiple properties of these matrices, including mechanical properties, cell binding, and degradation, is possible. We will culture human mesenchymal stem cells (hMSCs) in these matrices under both static and dynamic conditions, and employ a suite of oscillatory rheology, tensile testing, and high-frequency torsional-wave methods to characterize the mechanical properties of cell-encapsulated constructs. Histological, immunohistological, western blot, and gene expression techniques will be employed to confirm the differentiation of hMSCs and the production of vocal fold extracellular matrix. These studies will inform our choices of cell/materials constructs for injection into the vocal folds of rabbits to ameliorate vocal fold scarring. Our investigations wil thus contribute to the development of methods to characterize and culture materials at high frequencies, as well as yield a new class of materials that may optimize the regeneration of vocal fold tissue. Our approaches ultimately will be useful as a general platform in the design of materials for mechanically demanding regenerative medicine applications.
描述(由申请人提供):发声受损对个人健康和保健、社会和职业功能以及社会生产力具有重大影响;据估计,在美国,仅教师的声音问题每年造成的社会成本就达 25 亿美元。然而,用于治疗声带疾病的材料的开发受到声带严格的机械要求的阻碍,其中包括在高达 1000Hz 的频率下维持变形的能力,以及在瞬时拉伸高达 200% 后完全反冲的能力。迄今为止,尽管在声带组织工程材料支架的开发方面做出了广泛的努力,但尚未发现具有所需机械性能的材料。我们提出了解决这个问题的综合生物工程方法。我们将采用基于昆虫蛋白 Resilin 的新型弹性支架,它是昆虫发声、跳跃和飞行器官中的主要能量储存,并在高达 4000Hz 的频率变形后表现出无与伦比的弹性(拉伸后恢复)。我们将采用模块化重组方法来生成节肢动物样多肽(RLP),这些多肽可以被设计为携带生物活性结构域,而不损害节肢动物结构域的机械特性,并且可以独立调节这些基质的多种特性,包括机械特性、细胞结合和降解。我们将在静态和动态条件下在这些基质中培养人间充质干细胞(hMSC),并采用一套振荡流变学、拉伸测试和高频扭转波方法来表征细胞封装结构的机械性能。将采用组织学、免疫组织学、蛋白质印迹和基因表达技术来确认hMSC的分化和声带细胞外基质的产生。这些研究将为我们选择注射到兔子声带以改善声带疤痕的细胞/材料结构提供信息。因此,我们的研究将有助于开发高频表征和培养材料的方法,并产生一类可以优化声带组织再生的新型材料。我们的方法最终将成为机械要求较高的再生医学应用材料设计的通用平台。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Kristi L Kiick其他文献

Kristi L Kiick的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Kristi L Kiick', 18)}}的其他基金

Collagen-mediated approaches to improve the local delivery and hypothermic release of osteoarthritis therapeutics
胶原介导的方法改善骨关节炎治疗药物的局部递送和低温释放
  • 批准号:
    10595325
  • 财政年份:
    2023
  • 资助金额:
    $ 47.24万
  • 项目类别:
COATs: Collagen-mimetic peptide and therapeutic gene-modified collagens for cell-mediated healing of diabetic foot ulcers
COAT:胶原模拟肽和治疗性基因修饰胶原,用于细胞介导的糖尿病足溃疡愈合
  • 批准号:
    10459594
  • 财政年份:
    2021
  • 资助金额:
    $ 47.24万
  • 项目类别:
COATs: Collagen-mimetic peptide and therapeutic gene-modified collagens for cell-mediated healing of diabetic foot ulcers
COAT:胶原模拟肽和治疗性基因修饰胶原,用于细胞介导的糖尿病足溃疡愈合
  • 批准号:
    10629445
  • 财政年份:
    2021
  • 资助金额:
    $ 47.24万
  • 项目类别:
COATs: Collagen-mimetic peptide and therapeutic gene-modified collagens for cell-mediated healing of diabetic foot ulcers
COAT:胶原模拟肽和治疗性基因修饰胶原,用于细胞介导的糖尿病足溃疡愈合
  • 批准号:
    10317733
  • 财政年份:
    2021
  • 资助金额:
    $ 47.24万
  • 项目类别:
Highly resilient, hydrophilic bioelastomers for engineering vocal fold tissue
用于工程声带组织的高弹性、亲水性生物弹性体
  • 批准号:
    8811116
  • 财政年份:
    2012
  • 资助金额:
    $ 47.24万
  • 项目类别:
Highly resilient, hydrophilic bioelastomers for engineering vocal fold tissue
用于工程声带组织的高弹性、亲水性生物弹性体
  • 批准号:
    8445249
  • 财政年份:
    2012
  • 资助金额:
    $ 47.24万
  • 项目类别:
Highly resilient, hydrophilic bioelastomers for engineering vocal fold tissue
用于工程声带组织的高弹性、亲水性生物弹性体
  • 批准号:
    8295660
  • 财政年份:
    2012
  • 资助金额:
    $ 47.24万
  • 项目类别:
UDE COBRE: ARTIFICIAL GLYCOPROTEINS FOR APPLICATIONS IN MATERIALS AND BIOLOGY
UDE COBRE:用于材料和生物学应用的人造糖蛋白
  • 批准号:
    7960412
  • 财政年份:
    2009
  • 资助金额:
    $ 47.24万
  • 项目类别:
UDE COBRE: ARTIFICIAL GLYCOPROTEINS FOR APPLICATIONS IN MATERIALS AND BIOLOGY
UDE COBRE:用于材料和生物学应用的人造糖蛋白
  • 批准号:
    7720759
  • 财政年份:
    2008
  • 资助金额:
    $ 47.24万
  • 项目类别:
UDE COBRE: ARTIFICIAL GLYCOPROTEINS FOR APPLICATIONS IN MATERIALS AND BIOLOGY
UDE COBRE:用于材料和生物学应用的人造糖蛋白
  • 批准号:
    7381975
  • 财政年份:
    2006
  • 资助金额:
    $ 47.24万
  • 项目类别:

相似国自然基金

greenwashing behavior in China:Basedon an integrated view of reconfiguration of environmental authority and decoupling logic
  • 批准号:
  • 批准年份:
    2024
  • 资助金额:
    万元
  • 项目类别:
    外国学者研究基金项目

相似海外基金

RUI: CAS-MNP: Molecular Behavior at Colloidal/Aqueous Interfaces of Heterogeneous Nano- and Micro-Plastics - Binding Interactions and Effect of Aging
RUI:CAS-MNP:异质纳米和微米塑料胶体/水界面的分子行为 - 结合相互作用和老化效应
  • 批准号:
    2304814
  • 财政年份:
    2023
  • 资助金额:
    $ 47.24万
  • 项目类别:
    Standard Grant
Synthesis of Liquid-Crystalline Viologen Compounds with Flexible Ion Binding Sites and Their Photo-Responsive Behavior
具有灵活离子结合位点的液晶紫罗碱化合物的合成及其光响应行为
  • 批准号:
    17K14532
  • 财政年份:
    2017
  • 资助金额:
    $ 47.24万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Identifying Neurosensory Solutions to the Binding Problem in Animal Behavior
确定动物行为中约束问题的神经感觉解决方案
  • 批准号:
    1452831
  • 财政年份:
    2015
  • 资助金额:
    $ 47.24万
  • 项目类别:
    Continuing Grant
Development of cryptand molecules of multiple ligation with allosteric binding behavior
具有变构结合行为的多重连接的穴状配体分子的开发
  • 批准号:
    21550045
  • 财政年份:
    2009
  • 资助金额:
    $ 47.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The biological role of odorant-binding proteins in insect behavior
气味结合蛋白在昆虫行为中的生物学作用
  • 批准号:
    21688003
  • 财政年份:
    2009
  • 资助金额:
    $ 47.24万
  • 项目类别:
    Grant-in-Aid for Young Scientists (A)
Control of interfacial behavior through lipid domain formation, ligand-receptor binding and their synergetic effect
通过脂质域形成、配体-受体结合及其协同效应控制界面行为
  • 批准号:
    0828046
  • 财政年份:
    2008
  • 资助金额:
    $ 47.24万
  • 项目类别:
    Continuing Grant
Occurence and Mechanisms of Antibody-Antigen Allosteric Binding Behavior
抗体-抗原变构结合行为的发生和机制
  • 批准号:
    6727039
  • 财政年份:
    2004
  • 资助金额:
    $ 47.24万
  • 项目类别:
Evaluation of anticoagulant behavior of thrombin-inhibiting polymer with fibrinolytic factor-binding sites and application for biomaterials
具有纤溶因子结合位点的凝血酶抑制聚合物的抗凝行为评价及其在生物材料中的应用
  • 批准号:
    14580840
  • 财政年份:
    2002
  • 资助金额:
    $ 47.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
AnaIysis of dynamic behavior of ribonuclease upon ligand binding using high resolution NMR
使用高分辨率 NMR 分析配体结合时核糖核酸酶的动态行为
  • 批准号:
    12672088
  • 财政年份:
    2000
  • 资助金额:
    $ 47.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Shear Resisting Behavior of Reinforced Concrete Columns Under Biaxial Binding-Shear and Varying Axial Load
双轴绑剪和变轴荷载作用下钢筋混凝土柱的抗剪性能
  • 批准号:
    63460169
  • 财政年份:
    1988
  • 资助金额:
    $ 47.24万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了