Recombinant antibodies for cytoplasmic, nuclear and transmembrane proteins
细胞质、核和跨膜蛋白的重组抗体
基本信息
- 批准号:8796585
- 负责人:
- 金额:$ 32.93万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2014
- 资助国家:美国
- 起止时间:2014-09-30 至 2018-07-31
- 项目状态:已结题
- 来源:
- 关键词:AMPA ReceptorsAffinityAnimalsBindingBrainCell NucleusCellsCytoplasmic ProteinCytoskeletal ProteinsCytoskeletonDLG4 geneEpitopesEventFeedbackFibronectinsGenesGenomeGrantIn VitroIntegral Membrane ProteinKinesinLabelLaboratoriesLifeLocationMessenger RNAMethodsMitochondriaMolecularMolecular MotorsMotorNervous system structureNeuronsNuclear ProteinNuclear ProteinsOrganismPeptidesProtein RegionProteinsRecombinant AntibodyRegulationReportingStructureSynapsesSystemTechniquesTimeTranscription Factor AP-1Transcriptional RegulationWorkbasedensitydesignextracellulargephyrinin vivoinsightnovelpostsynapticprotein transportpublic health relevancereceptorreconstitutionresearch studytraffickingtranscription factor
项目摘要
DESCRIPTION (provided by applicant): The purpose of the experiments proposed in this grant is to generate recombinant antibody-like proteins (intrabodies) that can label specific proteins of all types, including cytoplasmic and nuclear proteins, and extracellular epitopes on transmembrane proteins. Recently we have shown the utility of the intrabody approach by generating probes that recognize the postsynaptic proteins Gephyrin and PSD95. These probes work efficiently and can be used to visualize endogenous proteins in living organisms. However, the design of these intrabodies places constraints on the targets against which they can be made. In particular, target proteins must be fixed to the cytoskeleton of the cell. Here we propose to develop a new strategy for generating intrabodies that does not place any requirements on the type of target protein. To establish the efficacy of this strategy we will generate intrabodies against the motor protein Kinesin 1, the transcription factor AP-1 and an extracellular epitope of the AMPA receptor GluA1.
描述(由申请人提供):本授权中提出的实验的目的是产生重组抗体样蛋白(胞内抗体),其可以标记所有类型的特异性蛋白,包括细胞质和核蛋白以及跨膜蛋白上的细胞外表位。最近,我们已经显示了效用的胞内抗体的方法产生的探针,识别突触后蛋白Gephyrin和PSD 95。这些探针工作效率高,可用于观察生物体内的内源性蛋白质。然而,这些胞内抗体的设计限制了它们可以针对的靶标。特别是,靶蛋白必须固定在细胞的细胞骨架上。在这里,我们建议开发一种新的策略,用于产生胞内抗体,不对靶蛋白的类型提出任何要求。为了建立这种策略的功效,我们将产生针对马达蛋白驱动蛋白1、转录因子AP-1和AMPA受体GluA 1的细胞外表位的胞内抗体。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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DONALD B ARNOLD其他文献
DONALD B ARNOLD的其他文献
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{{ truncateString('DONALD B ARNOLD', 18)}}的其他基金
Photoactivatable systems for controlling transcription and ablating synapses.
用于控制转录和消融突触的光激活系统。
- 批准号:
9927247 - 财政年份:2020
- 资助金额:
$ 32.93万 - 项目类别:
Recombinant antibodies for cytoplasmic, nuclear and transmembrane proteins
细胞质、核和跨膜蛋白的重组抗体
- 批准号:
9113665 - 财政年份:2014
- 资助金额:
$ 32.93万 - 项目类别:
Dynamic mapping of the complete synaptome using recombinant probes
使用重组探针动态绘制完整突触组
- 批准号:
8754412 - 财政年份:2014
- 资助金额:
$ 32.93万 - 项目类别:
Dynamic mapping of the complete synaptome using recombinant probes
使用重组探针动态绘制完整突触组
- 批准号:
9327798 - 财政年份:2014
- 资助金额:
$ 32.93万 - 项目类别:
Recombinant antibodies for cytoplasmic, nuclear and transmembrane proteins
细胞质、核和跨膜蛋白的重组抗体
- 批准号:
8932846 - 财政年份:2014
- 资助金额:
$ 32.93万 - 项目类别:
Recombinant antibodies for cytoplasmic, nuclear and transmembrane proteins
细胞质、核和跨膜蛋白的重组抗体
- 批准号:
9293372 - 财政年份:2014
- 资助金额:
$ 32.93万 - 项目类别:
Molecular probes to visualize endogenous synaptic proteins in vivo
体内内源性突触蛋白可视化的分子探针
- 批准号:
8598703 - 财政年份:2013
- 资助金额:
$ 32.93万 - 项目类别:
Molecular probes to visualize endogenous synaptic proteins in vivo
体内内源性突触蛋白可视化的分子探针
- 批准号:
9038465 - 财政年份:2013
- 资助金额:
$ 32.93万 - 项目类别:
Molecular probes to visualize endogenous synaptic proteins in vivo
体内内源性突触蛋白可视化的分子探针
- 批准号:
9248440 - 财政年份:2013
- 资助金额:
$ 32.93万 - 项目类别:
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