An Integrated Cross-Correlative Environmental Specimen Holder Platform for Optica

Optica 的集成互相关环境样品架平台

• 批准号: 8738682
• 负责人: Daan Hein Alsem
• 金额: $ 50万
• 依托单位: HUMMINGBIRD PRECISION MACHINE COMPANY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-30 至 2016-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8738682
• 关键词: Biological; Biological Process; Biological Sciences; Cell physiology; Cells; Cellular Structures; Ceramics; Charge; Containment; Development; Documentation; Dose; Electron Microscope; Electron Microscopy; Electrons; Environment; Environmental Engineering technology; Evaluation; Film; Freezing; Frequencies; Gases; Government; Health Sciences; Image; Incubated; Individual; Instruction; Laboratories; Light Microscope; Liquid substance; Location; Macromolecular Complexes; Marketing; Microfluidics; Microscope; Microscopy; Modern Medicine; Morphologic artifacts; Persons; Pharmaceutical Preparations; Phase; Positioning Attribute; Preparation; Regulation; Research; Research Personnel; Resolution; Sampling; Scanning; Science; Solutions; Specimen; Structure; Surface; System; Techniques; Temperature; Testing; Whole Organism; Work; abstracting; comparative; cryogenics; design; electron radiation; empowered; imaging modality; insight; light microscopy; nanoscale; prototype; public health relevance; research study; seal; single molecule; transmission process

DESCRIPTION (provided by applicant): To gain a comprehensive understanding of cellular structures and function, correlative light and electron microscopy has been widely utilized to facilitate the acquisition of both macroscopic view of the whole organism and the localization of single molecules or macromolecular complexes. However, current correlative microscopy solutions require extensive sample preparation before biological specimens can be investigated using EM, which potentially alters the relevant structures and induces image artifacts. Therefore, this Phase II project is aimed to develop and bring to market a universal continuous-flow environmental specimen holder system for the light microscope (LM) and the scanning and transmission electron microscope (SEM and TEM) with a biofunctionable sample substrate and a micron-precision location reference system for correlative microscopy to take advantage of multiple imaging modalities spanning a range of spatial scales and frequencies. This powerful and versatile correlative microscopy specimen holder platform will enable environmental LM and EM, allow accurate temperature regulation (from below 0 C to 100 C), and empower microfluidic experiments conducted inside the chamber, where biological samples are incubated or drug treated. It will also allow sample imaging across LM, SEM, and TEM platforms without additional manipulation. In Phase I we have successfully created a working prototype of the system. The proposed cross-correlated specimen holder platform offers the capability to transform any existing individual microscopes into a complete correlative microscopy system that permits the entire microscopy field to join forces to not only uncover many insights and research the most urgent advances in modern medicine, but more importantly to open new doors and offer opportunities to the entire field of life and health sciences.

描述（由申请人提供）：为了全面了解细胞结构和功能，相关的光和电子显微镜已被广泛用于促进对整个生物体的宏观视图的获取以及单分子或单分子复合物的定位。但是，当前的相关显微镜解决方案需要大量的样品制备，然后才能使用EM研究生物样品，这可能会改变相关的结构并诱导图像伪影。因此，该第二阶段项目的目的是开发和推销用于光学显微镜（LM）的通用连续流环境样品持有器系统，以及具有可实现多个成像模量范围范围范围范围范围范围的相关位置的扫描和传输电子显微镜（SEM和TEM），并具有可实现生物功能的样品底物和可相关显微镜的微观位置参考系统。这个功能强大且多功能相关的显微镜标本固定器平台将使环境LM和EM能够进行准确的温度调节（从低于0 C至100 C），并赋予在室内进行的微流体实验，并在其中进行生物样品，并在其中孵育或药物处理生物学样品。它还将允许在LM，SEM和TEM平台上进行样品成像，而无需其他操纵。在第一阶段，我们成功地创建了系统的工作原型。拟议的跨相关标本架平台提供了将任何现有的单个显微镜转换为完整的相关显微镜系统的能力，该系统使整个显微镜领域都可以连接起来，不仅要揭开许多见解，并研究了许多现代医学的最紧迫进步，而且更重要的是，为整个生活和健康科学领域提供了新的新门，并为整个领域提供机会。