An Integrated Cross-Correlative Environmental Specimen Holder Platform for Optica

Optica 的集成互相关环境样品架平台

• 批准号: 8123925
• 负责人: Daan Hein Alsem
• 金额: $ 15.12万
• 依托单位: HUMMINGBIRD PRECISION MACHINE COMPANY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-30 至 2012-03-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8123925
• 关键词: Area; Biological; Biological Process; Biological Sciences; Cell physiology; Cells; Cellular Structures; Containment; Cytoskeleton; Electric Wiring; Electron Microscope; Electron Microscopy; Ensure; Environment; Film; Freezing; Frequencies; Gases; Health Sciences; Heating; Housing; Image; In Situ; Incubated; Individual; Investigation; Light Microscope; Liquid substance; Location; Macromolecular Complexes; Membrane; Microfluidics; Microscope; Microscopy; Modern Medicine; Morphologic artifacts; Optics; Pharmaceutical Preparations; Phase; Positioning Attribute; Preparation; Regulation; Research; Resolution; Sampling; Scanning; Secure; Solutions; Specimen; Structure; Surface; System; Techniques; Temperature; Vacuum; Whole Organism; abstracting; base; cryogenics; empowered; feeding; imaging modality; insight; interest; light microscopy; nanoscale; research study; single molecule; transmission process

DESCRIPTION (provided by applicant): To gain a comprehensive understanding of cellular structures and function, correlative light and electron microscopy has been widely utilized to facilitate the acquisition of both macroscopic view of the whole organism and the localization of single molecules or macromolecular complexes. However, current correlative microscopy solutions require extensive sample preparation before biological specimens can be investigated using EM, which potentially altering the relevant structures and inducing image artifacts. Therefore, this project is aimed to develop a universal continuous-flow environmental specimen holder system for the light microscope (LM), the scanning and transmission electron microscope (SEM and TEM) with biofunctionable sample substrate and micron- precision location reference system for correlative microscopy to take advantage of multiple imaging modalities spanning a range of spatial scales and frequencies. This powerful and versatile correlative microscopy specimen holder platform enables environmental LM and EM, accurate temperature regulation (20:C to 80:C), and empowers microfluidic experiments being conducted inside the chamber, where biological samples are incubated or drug treated. It also allows sample imaging across LM, SEM and TEM platforms without additional manipulation. The proposed cross- correlated specimen holder platform offers the capability to transform any existing individual microscopes into the most complete correlative microscopy system that permits the entire microscopy field joining forces to not only uncover a great deal of insights and research the most urgent advances in modern medicine, but more importantly open new doors and opportunities to the entire life and health sciences. PUBLIC HEALTH RELEVANCE: An Integrated Cross-Correlative Environmental Specimen Holder Platform for Optical, Scanning and Transmission Electron Microscopes Relevance The environmental cross-correlative light and electron microscopy characterization techniques that will be developed in this project will have a direct impact on the understanding of biological processes. It targets the cross-correlative nanometer-scale imaging of biological structures in their native liquid environment, as well as the ability to observe biological processes with high resolution as they are occurring. This new correlative approach, which represents a giant leap forward compared to current correlative microscopy techniques using cryogenic freezing, will be used to gain a comprehensive understanding of cellular structures and function.

描述（由申请人提供）：为了全面了解细胞结构和功能，相关的光和电子显微镜已被广泛用于促进对整个生物体的宏观视图的获取以及单分子或单分子复合物的定位。但是，当前的相关显微镜解决方案需要大量的样品制备，然后才能使用EM研究生物样品，这可能会改变相关结构并诱导图像伪影。因此，该项目的目的是开发用于光学显微镜（LM）的通用连续环境标本持有器系统，扫描和传输电子显微镜（SEM和TEM），具有可生物功能的样品底物以及相关显微镜的微观固定位置参考系统，以利用多个成像模态范围范围范围范围和频率范围。这个功能强大且多才多艺的相关样品架平台可以使环境LM和EM，准确的温度调节（20：C至80：C），并赋予在室内进行的微流体实验，并在室内进行生物样品，并处理过生物学样品或药物处理。它还允许跨LM，SEM和TEM平台进行样品成像，而无需其他操纵。拟议的交叉相关标本持有人平台提供了将任何现有的单个显微镜转换为最完整的相关显微镜系统的能力，该系统允许整个显微镜磁场联合起色不仅可以揭示大量的见解，并研究现代医学的最紧迫进步，而且更重要的是，更重要的是为整个生活和健康科学开放新的门和机会。 公共卫生相关性：用于光学，扫描和传输电子显微镜相关性的综合跨性能环境标本架平台环境交叉功能光和电子显微镜表征技术将在该项目中开发，将对对生物学过程的理解产生直接影响。它针对生物学结构在其天然液体环境中的生物结构的互相关纳米尺度成像，以及在发生高分辨率的生物过程中的能力。与使用低温冻结相比，这种新的相关方法代表了与当前相关显微镜技术相比，它将用于对细胞结构和功能的全面理解。