Research on inhalation toxicology of environmental chemicals

环境化学品吸入毒理学研究

• 批准号: 8807775
• 负责人: LARRY BOWEN
• 金额: $ 695.47万
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-01-05 至 2014-01-04
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8807775
• 关键词: Acids; Animals; Autopsy; Breathing; Chemicals; Cimicifuga racemosa; Contractor; Contracts; Diacetyl; Funding; Histopathology; Inhalation Toxicology; Intramural Research; Intraperitoneal Injections; Liquid substance; Maintenance; Molecular Analysis; Mouse Strains; Mus; National Institute of Environmental Health Sciences; National Toxicology Program; Oropharyngeal; Proteins; Protocols documentation; Rat Strains; Rattus; Research; Research Personnel; Research Support; Respiratory Tract Diseases; Stem cells; System; Testing; Tissues; Toluidines; Tungsten; animal facility; cigarette smoking; environmental chemical; indium phosphide; indium tin oxide; intravenous injection; pulmonary function; tetrabromobisphenol A

During FY2014 approximately 22 studies were conducted for 7investigators in support of NTP research and 3 studies for 2 investigators in support of DIR research. Approximately 2000 animals (6 mouse strains and 4 rat strains) were received and maintained at the Inhalation Facility for these studies. Of these animals aproximately 1,000 were purchased using contract funds and 1,000 were received as transfers from the EPA animal facility and the NIEHS south campus animal facility. Animals were treated with approximately 18 different test agents (hypercapnic mixtures, 2,3-butanedione, 2,3-pentanedione, indium phosphide, indium-tin-oxide, perfluorooctanoic acid, tungsten, tungsten suboxide, tetrabromobisphenol A, DE-71, p-toluidine, DMPT, BDE47, black cohosh, cigarette smoke, and with stem cells from chemical- treated mice. Test agents were administered by whole-body inhalation, intratracheal instillation, oropharyngeal aspiration, intravenous injection, intraperitoneal injection, and gavage. In addition to animal maintenance and treatment, technicians also performed necropsies, collected bronchoalveolar fluid, and tissues for histopathology or molecular analysis, conducted protein and LDH analyses and evaluated pulmonary function using the Flexivent and Buxco systems.

2014财年期间，7名研究者开展了约22项研究以支持NTP研究，2名研究者开展了3项研究以支持NTP研究。接收了约2000只动物（6只小鼠品系和4只大鼠品系），并将其保存在吸入机构用于这些研究。在这些动物中，约1，000只是使用合同资金购买的，1，000只是从EPA动物机构和NIEHS南校区动物机构转移过来的。用大约18种不同的测试剂（高碳酸混合物、2，3-丁二酮、2，3-戊二酮、磷化铟、氧化铟锡、全氟辛酸、钨、低氧化钨、四溴双酚A、DE-71、对甲苯胺、DMPT、BDE 47、黑升麻、香烟烟雾）和来自化学处理的小鼠的干细胞处理动物。通过全身吸入、气管内滴注、口咽抽吸、静脉内注射、腹膜内注射和灌胃给予测试试剂。除动物维护和处理外，技术人员还进行尸检，收集支气管肺泡液和组织用于组织病理学或分子分析，进行蛋白质和LDH分析，并使用Atriovent和Buxco系统评价肺功能。