Research on inhalation toxicology of environmental chemicals

环境化学品吸入毒理学研究

• 批准号: 9004254
• 负责人: LARRY BOWEN
• 金额: $ 583.83万
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-01-05 至 2016-01-04
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9004254
• 关键词: Animals; Autopsy; Blood; Breathing; Cimicifuga racemosa; Contractor; Contracts; Diacetyl; Free Radicals; Funding; Health; Histopathology; Hyperoxia; Inhalation Toxicology; Intramural Research; Liquid substance; Mainstreaming; Maintenance; Measures; Methods; Molecular Analysis; Mouse Strains; Mus; National Institute of Environmental Health Sciences; National Toxicology Program; Nose; Ovalbumin; Ozone; Phenobarbital; Polychlorinated Biphenyls; Proteins; Protocols documentation; Rat Strains; Rattus; Research; Research Personnel; Research Support; Respiratory Tract Diseases; Testing; Tissues; Tungsten; animal facility; cigarette smoking; cumene; environmental chemical; environmental enrichment for laboratory animals; indium phosphide; indium tin oxide; intravenous injection; neonate; postnatal; pregnant

During FY2015 approximately 18 studies were conducted for 6 investigators in support of NTP research and 3 studies for 3 investigators in support of DIR research. Approximately 1,266 animals (3 mouse strains and 2 rat strains) were received and maintained at the Inhalation Facility for these studies. Of these animals 1,194 were purchased using contract funds and 19 were received as transfers from the NIEHS south campus animal facility. Animals were treated with approximately 13 different test agents by whole body inhalation (hyperoxia, ozone, 2,3-butanedione, 2,3-pentanedione), nose-only inhalation (4-ethyltoluene, cumene, 3-ethyltoluene), intratracheal instillation (tungsten, tungsten suboxide, indium phosphide, indium-tin-oxide, ovalbumin), intravenous injection (promiR-29), and gavage (BDE-47, phenobarbital, PCB-126, black cohosh). Four gavage studies involved treatment of pregnant dams and neonates through postnatal day 22. A study was conducted to evaluate the effects of environmental enrichment on animal health. A study also was conducted to evaluate a method for measuring free radicals in mainstream cigarette smoke (no animals). In addition to animal maintenance and treatment, technicians also performed necropsies, collected bronchoalveolar fluid, blood and tissues for histopathology or molecular analysis, and conducted protein and LDH analyses.

在2015财年期间，为支持NTP研究，6名研究者进行了约18项研究，为支持NTP研究，为3名研究者进行了3项研究。 接收了约1，266只动物（3种小鼠品系和2种大鼠品系），并将其保存在吸入机构用于这些研究。 在这些动物中，1，194只是使用合同资金购买的，19只是从NIEHS南校区动物设施转移过来的。 通过全身吸入用大约13种不同的测试试剂处理动物（高氧、臭氧、2，3-丁二酮、2，3-戊二酮），仅鼻吸入（4-乙基甲苯、枯烯、3-乙基甲苯），气管内滴注（钨、低价氧化钨、磷化铟、氧化铟锡、卵清蛋白），静脉注射（promiR-29）和灌胃（BDE-47、苯巴比妥、PCB-126、黑升麻）。 4项灌胃研究涉及妊娠母鼠和新生儿的给药，直至出生后第22天。 进行了一项研究，以评估环境富集对动物健康的影响。 还进行了一项研究，以评估测量主流香烟烟雾中自由基的方法（无动物）。 除动物维护和处理外，技术人员还进行尸检，收集支气管肺泡液、血液和组织用于组织病理学或分子分析，并进行蛋白质和LDH分析。