Research on inhalation toxicology of environmental chemicals

在2015财年，为支持NTP研究的6名研究者进行了大约18项研究，为支持DIR研究的3名研究者进行了3项研究。大约1,266只动物（3个小鼠品系和2个大鼠品系）被接收并维持在吸入设施进行这些研究。在这些动物中，1194只是用合同资金购买的，19只是从NIEHS南校区动物设施转移过来的。实验采用全身吸入（高氧、臭氧、2,3-丁二酮、2,3-戊二酮）、纯鼻吸入（4-乙基甲苯、异丙烯、3-乙基甲苯）、气管内滴注（钨、亚氧化钨、磷化铟、氧化铟锡、卵白蛋白）、静脉注射（promiR-29）和灌胃（BDE-47、苯巴比妥、多氯联苯-126、黑升麻）等方法对动物进行了大约13种不同的试验剂处理。四项灌胃研究涉及孕鼠和新生儿在出生后22天的治疗。对环境富集对动物健康的影响进行了研究。还进行了一项研究，以评估一种测量主流香烟烟雾中自由基的方法（无动物）。除了动物维护和治疗外，技术人员还进行了尸检，收集了支气管肺泡液、血液和组织进行组织病理学或分子分析，并进行了蛋白质和LDH分析。

During FY2015 approximately 18 studies were conducted for 6 investigators in support of NTP research and 3 studies for 3 investigators in support of DIR research. Approximately 1,266 animals (3 mouse strains and 2 rat strains) were received and maintained at the Inhalation Facility for these studies. Of these animals 1,194 were purchased using contract funds and 19 were received as transfers from the NIEHS south campus animal facility. Animals were treated with approximately 13 different test agents by whole body inhalation (hyperoxia, ozone, 2,3-butanedione, 2,3-pentanedione), nose-only inhalation (4-ethyltoluene, cumene, 3-ethyltoluene), intratracheal instillation (tungsten, tungsten suboxide, indium phosphide, indium-tin-oxide, ovalbumin), intravenous injection (promiR-29), and gavage (BDE-47, phenobarbital, PCB-126, black cohosh). Four gavage studies involved treatment of pregnant dams and neonates through postnatal day 22. A study was conducted to evaluate the effects of environmental enrichment on animal health. A study also was conducted to evaluate a method for measuring free radicals in mainstream cigarette smoke (no animals). In addition to animal maintenance and treatment, technicians also performed necropsies, collected bronchoalveolar fluid, blood and tissues for histopathology or molecular analysis, and conducted protein and LDH analyses.