Histology and Comparative Pathology Core

组织学和比较病理学(CORE C)将提供U19 CMCR的四个项目，授予一个全面的组织病理学设施，帮助研究人员进行所需的组织学分析，以分析干细胞在改善辐射诱导的胃肠道综合征(RIGS)、辐射诱导的造血综合征(RIHS)和辐射诱导的皮肤综合征(RICS)的结局中所起的作用。 该核心将就样品的准备和处理以及可用于不同研究的技术和程序提供咨询。中心将为表型分析提供尸检和组织病理学服务，以及全面的组织学服务 该核心提供的服务将包括：方案设计、尸检，包括组织固定、组织进行石蜡切片、冷冻包埋、标准和专门染色以及免疫组织化学分析。它还将包括利用标准化分析方法对组织样本进行组织病理学评估。 核心的关键人员将是主任和组织学技术人员。主任将负责监督核心的所有活动，就不同的方法和程序提供咨询意见，解释结果，并编写组织病理学报告和对数据的解释。技术人员将为手头的研究准备和处理适当的组织样本，执行一些专门的技术和程序(例如免疫组织化学和特殊染色)。 意义：适当的组织样品准备(固定、加工、修剪和染色)对本计划项目中的所有项目都是至关重要的，因为它允许研究干细胞技术对辐射诱导损伤结果的体内影响。

The Histology and Comparative Pathology (Core C) will provide the four projects of this U19 CMCR grant a comprehensive histopathology facility that will assist the investigators in the histological analysis required for the investigators to analyze the role of stem cells in improving the outcome of radiation induce gastrointestinal syndrome (RIGS), Radiation induced hematopoietic syndrome (RIHS), and Radiation-induced cutaneous syndrome (RICS). The core will provide advice on preparation and processing of samples, as well as on the techniques and procedures available for the different studies. The Core will provide necropsy and histopathology services for phenotypic analysis along with a full range of histological services The services offered by the core will include: protocol design, necropsy, including tissue fixation, tissue proceeding to paraffin block, frozen embedding, standard and specialized stains and immunohistochemieal analyses. It will also include histopathological evaluation of tissue samples utilizing a standardized method of analysis. The key personnel of the core will be the director and the histology technical staff. The director will be responsible for supervising all the activities of the core, offering advice on the different methods and procedures, interpretation of the results, and generating histopathology reports and interpretations of the data. The technical staff will prepare and process tissue samples as appropriate for the study at hand, perform some of the specialized techniques and procedures (e.g. immunohistochemistry and special stains). Significance: Appropriate tissue sample preparation (fixation, processing, trimming and staining) is essential to all projects in this Program Project as it allow the study of the in vivo impact of stem cell technologies on the outcome of radiation-induced injury.