Histology and Comparative Pathology Core

组织学和比较病理学核心

• 批准号: 8013182
• 负责人: RANI S SELLERS
• 金额: $ 17.15万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2015-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8013182
• 关键词: Animal Model; Animals; Autopsy; Comparative Pathology; Control Groups; Cutaneous; Data; Freezing; Goals; Grant; Hand; Hematopoietic; Histology; Histopathology; Histopathology Facility; Human Resources; Image; Immunohistochemistry; Injury; Intestines; Lesion; Maintenance; Methods; Outcome; Paraffin; Preparation; Procedures; Process; Protocols documentation; Radiation; Radiation Syndromes; Reagent; Reporting; Research Personnel; Role; Sampling; Services; Staining method; Stains; Stem cells; Syndrome; Techniques; Tissue Fixation; Tissue Sample; Tissues; Training; design; fluorescence microscope; gastrointestinal; improved; in vivo; member; programs; research study; sample fixation; skin lesion; stem cell technology; stem cell therapy

The Histology and Comparative Pathology (Core C) will provide the four projects of this U19 CMCR grant a comprehensive histopathology facility that will assist the investigators in the histological analysis required for the investigators to analyze the role of stem cells in improving the outcome of radiation induce gastrointestinal syndrome (RIGS), Radiation induced hematopoietic syndrome (RIHS), and Radiation-induced cutaneous syndrome (RICS). The core will provide advice on preparafion and processing of samples, as well as on the techniques and procedures available for the different studies. The Core will provide necropsy and histopathology services for phenotypic analysis along with a full range of histological services The services offered by the core will include: protocol design, necropsy, including tissue fixation, tissue proceeding to paraffin block, frozen embedding, standard and specialized stains and immunohistochemieal analyses. It will also include histopathological evaluafion of tissue samples utilizing a standardized method of analysis. The kev personnel of the core will be the director and the histology technical staff. The director will be responsible for supervising all the activities of the core, offering advice on the different methods and procedures, interpretafion of the results, and generating histopathology reports and interpretations of the data. The technical staff will prepare and process tissue samples as appropriate for the study at hand, perform some of the specialized techniques and procedures (e.g. immunohistochemistry and special stains). Significance: Appropriate tissue sample preparation (fixation, processing, trimming and staining) is essential to all projects in this Program Project as it allow the study of the in vivo impact of stem cell technologies on the outcome of radiation-induced injury.

组织学和比较病理学（核心C）将提供本U19 CMCR的四个项目 授予一个全面的组织病理学设施，将协助研究人员在组织学分析 研究人员需要分析干细胞在改善辐射诱导的结果中的作用， 胃肠道综合征（RIGS），放射诱导的造血综合征（RIHS），和 放射性皮肤综合征（RICS）。 该中心将提供关于样品的收集和处理以及技术方面的建议。 以及不同研究可用的程序。中心将提供尸检和组织病理学检查 表型分析服务沿着全方位的组织学服务 核心提供的服务将包括：方案设计，尸检，包括组织固定，组织 进行石蜡块、冷冻包埋、标准和专用染色， 化学分析。它还将包括组织样本的组织病理学评价， 标准化的分析方法。 核心的kev人员将是主任和组织学技术人员。导演将是 负责监督核心的所有活动，就不同的方法提供咨询意见， 程序，结果的解释，以及生成组织病理学报告和数据的解释。 技术人员将根据当前研究的需要制备和处理组织样本， 专业技术和程序（如免疫组织化学和特殊染色）。 意义：适当的组织样本制备（固定、处理、修剪和染色） 对本计划项目中的所有项目至关重要，因为它允许研究干细胞在体内的影响 技术对辐射损伤后果的影响。