Elucidating a molecular pathway for synaptic vesicle maintenance and degradation

阐明突触小泡维持和降解的分子途径

• 批准号: 8765805
• 负责人: Clarissa Leigh Waites
• 金额: --
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-01-01 至 2014-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8765805
• 关键词: Affect; Alzheimer' s Disease; American; Biochemistry; Caring; Carrier Proteins; Cell Death; Cell physiology; Communication; Complex; Data; Degradation Pathway; Disease; Drosophila genus; Elderly; Electron Microscopy; Endocytosis; Etiology; Eukaryotic Cell; Event; Exhibits; Future; Gene Expression Profiling; Glutamates; Goals; Hippocampus (Brain); Homeostasis; Huntington Disease; Image; Immunofluorescence Microscopy; Life; Lysosomes; Maintenance; Mediating; Mediator of activation protein; Membrane Protein Traffic; Membrane Proteins; Molecular; Monitor; Monomeric GTP-Binding Proteins; Multivesicular Body; Nerve Degeneration; Nervous system structure; Neurodegenerative Disorders; Neuromuscular Junction; Neurons; Parkinson Disease; Pathway interactions; Phenotype; Physiological; Process; Productivity; Property; Protein Biosynthesis; Proteins; RNA Interference; Recycling; Regulation; Role; Signal Transduction; Site; Sorting - Cell Movement; Structure; Synapses; Synaptic Vesicles; System; Techniques; Testing; Ubiquitination; Vesicle; Work; age related; base; cost; insight; loss of function; neuronal cell body; neurotransmitter release; novel; presynaptic; protein degradation; protein function; public health relevance; rab GTP-Binding Proteins; research study; response; trafficking; ubiquitin-protein ligase

DESCRIPTION (provided by applicant): Synapses not only support stable signaling between neurons over the course of months to years, but they also have the remarkable capacity to rapidly change their response properties based on inputs from other neurons. These functions depend upon the exquisite regulation of protein synthesis, trafficking, and degradation, collectively termed 'protein homeostasis' or 'proteostasis'. A critical aspect of synaptic proteostasis is the maintenance of synaptic vesicle (SV) pools within presynaptic boutons ('SV-stasis'). SV pools support the sustained release of neurotransmitter by maintaining a local reservoir of proteins to facilitate vesicle recycling [3, 4]. Moreover, SV loss precedes synapse degeneration and cell death in many forms of neurodegeneration [5- 8], suggesting that disruption of SV-stasis triggers more widespread degenerative processes. Understanding how SV-stasis is maintained and regulated will therefore provide critical insights into the etiology of neurodegenerative diseases such as Alzheimer's and Parkinson's. However, while the molecules that regulate SV exo/endocytosis have been extensively studied, those that regulate SV maintenance and degradation remain almost entirely unknown. The overall goal of this proposal is to elucidate the molecular pathway that mediates SV protein degradation in mammalian glutamatergic neurons. Our previous studies have identified three potential components of this pathway (the E3 ubiquitin ligase Siah1, the endosomal sorting complex required for transport (ESCRT) system, and the small GTPase Rab35), and this project will evaluate their roles in facilitating SV protein degradation. We will further assess whether pathological activation of this pathway disrupts SV-stasis and triggers synapse degeneration. In Aim 1, we will test whether Siah1 is a key mediator of SV protein ubiquitination. We propose that its over-activation induces hyper-ubiquitination and degradation of SV proteins, followed by SV loss and synapse degeneration, while its inhibition or knockdown leads to increased SV pool size and stability. To test this hypothesis, we will use biochemistry, immunofluorescence microscopy, live imaging, and electron microscopy to assess effects of Siah1 gain- or loss-of-function on SV protein abundance and turnover. In Aim 2, we will determine whether ubiquitinated SV proteins are targeted to lysosomes via the ESCRT pathway. Here, we will use the techniques from Aim 1 together with knockdown of key ESCRT proteins to examine whether the ESCRT pathway is essential for SV protein degradation under normal and pathological conditions. In Aim 3, we will evaluate whether Rab35 mediates SV protein degradation and functions upstream of the ESCRT pathway. We will again use techniques from Aim 1, together with Rab35 gain- and loss-of-function, to reveal whether Rab35 sorts SV membrane proteins into endosomal intermediates, promoting their entry into an ESCRT-dependent degradative pathway. Together, these studies will provide novel insights into how SV-stasis is maintained, and how its dysregulation contributes to synapse degeneration and the etiology of neurodegenerative disease.

描述（由申请人提供）：突触不仅在数月至数年的过程中支持神经元之间稳定的信号传递，而且它们还具有根据其他神经元的输入快速改变其反应特性的显着能力。这些功能依赖于对蛋白质合成、运输和降解的精细调节，统称为“蛋白质稳态”或“蛋白质稳态”。突触蛋白停滞的一个关键方面是突触前钮扣内突触囊泡（SV）池的维持（“SV停滞”）。SV池通过维持局部蛋白质储存库来促进囊泡循环，从而支持神经递质的持续释放[3,4]。此外，在许多形式的神经退行性变中，SV的丧失先于突触变性和细胞死亡[5- 8]，这表明SV停滞的破坏会引发更广泛的退行性过程。因此，了解sv -郁积是如何维持和调节的，将为了解其病因提供重要的见解