Acquisition of Octet Biolayer Interferometry system for MIT biophysics facility

为麻省理工学院生物物理设施采购 Octet Biolayer 干涉测量系统

• 批准号: 8640541
• 负责人: Barbara Imperiali
• 金额: $ 18.04万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-05-01 至 2015-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8640541
• 关键词: Avidity; Biological; Biological Process; Biology; Biophysics; Communities; Complex; Dissociation; Engineering; Equilibrium; Equipment; Faculty; Foundations; Funding; In Vitro; Interferometry; Life; Ligand Binding; Ligands; Location; Nucleic Acids; Process; Proteins; Reaction; Research; Running; Sampling; Solutions; Specificity; System; Time; high throughput analysis; instrument; instrumentation; macromolecule; professor; programs; small molecule

PROJECT SUMMARY All living processes are regulated by the dynamic interactions of proteins with small molecule ligands, partner proteins, nucleic acids, or other macromolecules. Instrumentation that enables quantitative analysis of the specificities and avidities of these interactions in vitro provides a physical foundation for understanding the intricate networks of reactions that contribute to complex biological processes. In this proposal, eleven faculty in the Departments of Biology and Biological Engineering at MIT request funding for the acquisition of an Octet RED96 instrument for the analysis of biomolecular interactions. This instrumentation exploits biolayer interferometry (BLI) to provide real time information on the binding of ligands in solution to immobilized molecules. The instrumentation accommodates a 96-well plate format for high- throughput analysis and is exceptionally versatile. Ligands can range from small molecules (>150 Da) up to very large protein or nucleic acid oligomers, and the acquisition of association and dissociation rate constants rapidly provides equilibrium constants over a broad dynamic range (10 pM - 1 mM) with very efficient sample requirements. Community access to hands-on use of this equipment will advance the pace and sophistication of multiple research programs and will be guaranteed by the central location of the instrument in the shared Biophysical Instrumentation Facility (BIF) in Building 68 of the MIT campus, which is run by a full-time technician (Ms. Debby Pheasant) and is directed by Professor Barbara Imperiali. The oversight committee for the acquisition and administration of all equipment in the BIF comprises Professors Barbara Imperiali (Chair), Robert Sauer, Amy E. Keating, Jacquin Niles and Ms. Debby Pheasant. !

项目概要 所有生命过程都受到蛋白质与小分子的动态相互作用的调节 配体、伴侣蛋白、核酸或其他大分子。仪器仪表可实现 体外这些相互作用的特异性和亲和力的定量分析提供了 理解复杂的反应网络的物理基础，这些反应有助于 复杂的生物过程。在这项提案中，生物系和生物系的 11 名教员 麻省理工学院的生物工程部门请求资助购买 Octet RED96 仪器 用于分析生物分子相互作用。该仪器利用生物层 干涉测量 (BLI) 可提供溶液中配体结合的实时信息 固定化分子。该仪器可容纳 96 孔板格式，用于高 吞吐量分析，用途广泛。配体可以是小分子 (>150 Da) 直至非常大的蛋白质或核酸寡聚体，并获得关联 和解离速率常数快速提供广泛动态范围内的平衡常数 范围（10 pM - 1 mM），样品要求非常高效。社区获得实践机会 使用该设备将提高多个研究项目的速度和复杂性 并将由仪器位于共享生物物理中心的中心位置来保证 麻省理工学院校园 68 号楼的仪器设施 (BIF)，由一名全职人员负责管理 技术员（Debby Pheasant 女士），由 Barbara Imperiali 教授指导。监督 BIF 内所有设备的采购和管理委员会包括 Barbara Imperiali 教授（主席）、Robert Sauer、Amy E. Keating、Jacquin Niles 和女士。 黛比雉鸡。 ！

项目成果

De Novo Discovery of High-Affinity Peptide Binders for the SARS-CoV-2 Spike Protein.

• DOI: 10.1021/acscentsci.0c01309
• 发表时间: 2021-01-27
• 期刊: ACS central science
• 影响因子: 18.2
• 作者: Pomplun S;Jbara M;Quartararo AJ;Zhang G;Brown JS;Lee YC;Ye X;Hanna S;Pentelute BL
• 通讯作者: Pentelute BL

Generation of Thermally Stable Affinity Pairs for Sensitive, Specific Immunoassays.

生成热稳定亲和对，用于灵敏、特异性的免疫测定。

• DOI: 10.1007/978-1-0716-2285-8_21
• 发表时间: 2022
• 期刊: Methods in molecular biology (Clifton, N.J.)
• 作者: Corless,Elliot;Hao,Yining;Jia,Huan;Kongsuphol,Patthara;Tay,DousabelMY;Ng,SayYong;Sikes,HadleyD
• 通讯作者: Sikes,HadleyD

Salt Effect Accelerates Site-Selective Cysteine Bioconjugation.

• DOI: 10.1021/acscentsci.6b00180
• 发表时间: 2016-09-28
• 期刊: ACS CENTRAL SCIENCE
• 影响因子: 18.2
• 作者: Dai, Peng;Zhang, Chi;Welborn, Matthew;Shepherd, James J.;Zhu, Tianyu;Van Voorhis, Troy;Pentelute, Bradley L.
• 通讯作者: Pentelute, Bradley L.

Discovery of Nucleic Acid Binding Molecules from Combinatorial Biohybrid Nucleobase Peptide Libraries.

• DOI: 10.1021/jacs.0c08964
• 发表时间: 2020-11-18
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: Pomplun S;Gates ZP;Zhang G;Quartararo AJ;Pentelute BL
• 通讯作者: Pentelute BL

A reactive peptide interface for site-selective cysteine bioconjugation.

• DOI: 10.1039/d1cc00095k
• 发表时间: 2021-04-04
• 期刊: Chemical communications (Cambridge, England)
• 作者: Tuang S ;Dieppa-Matos D ;Zhang C ;Shugrue CR ;Dai P ;Loas A ;Pentelute BL
• 通讯作者: Pentelute BL