Development and function in mucociliary epithelia
粘液纤毛上皮的发育和功能
基本信息
- 批准号:8714042
- 负责人:
- 金额:$ 37.85万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2013
- 资助国家:美国
- 起止时间:2013-08-15 至 2017-05-31
- 项目状态:已结题
- 来源:
- 关键词:AirAnimal ModelAnimalsApicalArchitectureAsthmaAutomobile DrivingBioinformaticsBiologicalBiological ModelsBiological ProcessBiologyBloodBreathingCarbon DioxideCellsCellular biologyChronic Obstructive Airway DiseaseCiliaComputational BiologyDataDefectDevelopmentDiseaseEpithelialEpitheliumExcisionGasesGene ExpressionGene TargetingGenesGeneticGenomicsGermGoalsHomeostasisImageLeadLifeLinkLung diseasesMammalsMesenchymal Stem CellsModelingMorphogenesisMucous body substanceOxygenPatientsPopulationPositioning AttributeProcessRFX regulatory factorRoleSpeedStructureSurfaceSystemTimeTissuesTransgenic Organismscell behaviorcell typecilium biogenesisfascinateflygenetic manipulationimprovedin vivoin vivo Modelin vivo imaginglung developmentlung repairmovienovelnovel strategiesparticleprogenitorpublic health relevanceregenerative therapyrepairedresearch studytooltranscription factor
项目摘要
DESCRIPTION (provided by applicant): The mucociliary epithelium holds a central position in both normal and pathological airway biology, as it provides both the interface for air exchange and the first line of defense against inhaled agents. Here, we will study the transcription factor RFX2, which we have shown is essential for two crucial aspects of mucociliary epithelial development and maturation. 1) Our preliminary data demonstrate an essential role for RFX2 in motile ciliogenesis. We will combine a novel model system, in vivo imaging, and a unique suite of genomic and bioinformatic approaches to study the role of RFX2 in the multi-ciliated cells. 2) Newly-born multi-ciliated cells arise from basally-located mesenchymal stem cells that must migrate apically and insert into the epithelium. This crucial process in mucociliary biology remains almost totally undefined, but we find that RFX2 is essential for this insertion. We will again combine genomics, bioinformatics and in vivo imaging to study the role of RFX2 in this context. By rapidly determining the functions of several new genes involved in distinct processes in mucociliary epithelial development, the aims in this project will provide critical new
depth to our understanding of these essential tissues. Moreover, by linking a single transcription factor to the control of two such disparate aspects of mucociliary epithelial biology, the experiments here will add crucial new breadth to our understanding as well. Impact: Experiments proposed here will lead to a more detailed understanding of the cell biology and genetics of mucociliary epithelia. The results will aid in the development of regenerative therapies aimed at repairing or restoring damaged tissue and improving mucus clearance in patients with airway disease.
描述(由申请人提供):粘膜纤毛上皮在正常和病理气道生物学中占据中心地位,因为它提供了空气交换的界面和对抗吸入剂的第一道防线。在这里,我们将研究转录因子RFX2,我们已经证明这是必不可少的两个关键方面的粘液纤毛上皮的发展和成熟。1)我们的初步数据表明RFX2在能动纤毛发生中起重要作用。我们将结合联合收割机一个新的模型系统,在体内成像,和一套独特的基因组和生物信息学的方法来研究RFX2在多纤毛细胞中的作用。2)新生的多纤毛细胞来自于位于基底的间充质干细胞,其必须向顶端迁移并插入上皮。在粘液纤毛生物学的这一关键过程仍然几乎完全不确定,但我们发现RFX2是必不可少的插入。我们将再次结合联合收割机基因组学,生物信息学和体内成像研究RFX2在这方面的作用。通过快速确定参与粘膜纤毛上皮发育不同过程的几个新基因的功能,该项目的目标将提供关键的新信息。
我们对这些重要组织的理解。此外,通过将单个转录因子与粘液纤毛上皮生物学的两个不同方面的控制联系起来,这里的实验也将为我们的理解增加至关重要的新广度。影响:这里提出的实验将导致更详细地了解粘膜纤毛上皮细胞的细胞生物学和遗传学。研究结果将有助于开发再生疗法,旨在修复或恢复受损组织并改善气道疾病患者的粘液清除。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
John B Wallingford其他文献
A new standard nomenclature for proteins related to Apx and Shroom
- DOI:
10.1186/1471-2121-7-18 - 发表时间:
2006-04-14 - 期刊:
- 影响因子:2.700
- 作者:
Olivier Hagens;Andrea Ballabio;Vera Kalscheuer;Jean-Pierre Kraehenbuhl;M Vittoria Schiaffino;Peter Smith;Olivier Staub;Jeff Hildebrand;John B Wallingford - 通讯作者:
John B Wallingford
John B Wallingford的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('John B Wallingford', 18)}}的其他基金
Control of collective cell movement by planar cell polarity signaling
通过平面细胞极性信号控制集体细胞运动
- 批准号:
10225582 - 财政年份:2020
- 资助金额:
$ 37.85万 - 项目类别:
Control of collective cell movement by planar cell polarity signaling
通过平面细胞极性信号控制集体细胞运动
- 批准号:
10704441 - 财政年份:2020
- 资助金额:
$ 37.85万 - 项目类别:
Control of collective cell movement by planar cell polarity signaling
通过平面细胞极性信号控制集体细胞运动
- 批准号:
10042185 - 财政年份:2020
- 资助金额:
$ 37.85万 - 项目类别:
Control of collective cell movement by planar cell polarity signaling
通过平面细胞极性信号控制集体细胞运动
- 批准号:
10622573 - 财政年份:2020
- 资助金额:
$ 37.85万 - 项目类别:
Control of collective cell movement by planar cell polarity signaling
通过平面细胞极性信号控制集体细胞运动
- 批准号:
10403992 - 财政年份:2020
- 资助金额:
$ 37.85万 - 项目类别:
Dynamics of vertebrate planar cell polarity proteins
脊椎动物平面细胞极性蛋白的动力学
- 批准号:
8986583 - 财政年份:2015
- 资助金额:
$ 37.85万 - 项目类别:
Control of collective cell movement by planar cell polarity signaling
通过平面细胞极性信号控制集体细胞运动
- 批准号:
9127983 - 财政年份:2015
- 资助金额:
$ 37.85万 - 项目类别:
Development and function in mucociliary epithelia
粘液纤毛上皮的发育和功能
- 批准号:
9099911 - 财政年份:2013
- 资助金额:
$ 37.85万 - 项目类别:
Development and function in mucociliary epithelia
粘液纤毛上皮的发育和功能
- 批准号:
10658656 - 财政年份:2013
- 资助金额:
$ 37.85万 - 项目类别:
相似海外基金
Quantification of Neurovasculature Changes in a Post-Hemorrhagic Stroke Animal-Model
出血性中风后动物模型中神经血管变化的量化
- 批准号:
495434 - 财政年份:2023
- 资助金额:
$ 37.85万 - 项目类别:
Bioactive Injectable Cell Scaffold for Meniscus Injury Repair in a Large Animal Model
用于大型动物模型半月板损伤修复的生物活性可注射细胞支架
- 批准号:
10586596 - 财政年份:2023
- 资助金额:
$ 37.85万 - 项目类别:
A Comparison of Treatment Strategies for Recovery of Swallow and Swallow-Respiratory Coupling Following a Prolonged Liquid Diet in a Young Animal Model
幼年动物模型中长期流质饮食后吞咽恢复和吞咽呼吸耦合治疗策略的比较
- 批准号:
10590479 - 财政年份:2023
- 资助金额:
$ 37.85万 - 项目类别:
Small animal model for evaluating the impacts of cleft lip repairing scar on craniofacial growth and development
评价唇裂修复疤痕对颅面生长发育影响的小动物模型
- 批准号:
10642519 - 财政年份:2023
- 资助金额:
$ 37.85万 - 项目类别:
Diurnal grass rats as a novel animal model of seasonal affective disorder
昼夜草鼠作为季节性情感障碍的新型动物模型
- 批准号:
23K06011 - 财政年份:2023
- 资助金额:
$ 37.85万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Longitudinal Ocular Changes in Naturally Occurring Glaucoma Animal Model
自然发生的青光眼动物模型的纵向眼部变化
- 批准号:
10682117 - 财政年份:2023
- 资助金额:
$ 37.85万 - 项目类别:
A whole animal model for investigation of ingested nanoplastic mixtures and effects on genomic integrity and health
用于研究摄入的纳米塑料混合物及其对基因组完整性和健康影响的整体动物模型
- 批准号:
10708517 - 财政年份:2023
- 资助金额:
$ 37.85万 - 项目类别:
A Novel Large Animal Model for Studying the Developmental Potential and Function of LGR5 Stem Cells in Vivo and in Vitro
用于研究 LGR5 干细胞体内外发育潜力和功能的新型大型动物模型
- 批准号:
10575566 - 财政年份:2023
- 资助金额:
$ 37.85万 - 项目类别:
Elucidating the pathogenesis of a novel animal model mimicking chronic entrapment neuropathy
阐明模拟慢性卡压性神经病的新型动物模型的发病机制
- 批准号:
23K15696 - 财政年份:2023
- 资助金额:
$ 37.85万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
The effect of anti-oxidant on swallowing function in an animal model of dysphagia
抗氧化剂对吞咽困难动物模型吞咽功能的影响
- 批准号:
23K15867 - 财政年份:2023
- 资助金额:
$ 37.85万 - 项目类别:
Grant-in-Aid for Early-Career Scientists