NRI: Paraellel, Independent Control of Microrobots for Microassembly of Tissues

NRI：并行、独立控制用于组织微组装的微型机器人

• 批准号: 8517118
• 负责人: Aaron Ohta
• 金额: $ 8.56万
• 依托单位: UNIVERSITY OF HAWAII AT MANOA
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-01 至 2015-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8517118
• 关键词: Address; Affect; Biological; Biological Sciences; Biomedical Research; Caring; Cell Separation; Cell model; Cells; Coculture Techniques; Communities; Computer software; Cultured Cells; Development; Dimensions; Engineering; Goals; Growth; Health; Human body; Hydrogels; In Vitro; Individual; Location; Magnetism; Medical; Medical Research; Methods; Microbubbles; Micromanipulation; Mission; National Institute of Biomedical Imaging and Bioengineering; Optical Methods; Optics; Organ; Pattern; Pharmacotherapy; Process; Protocols documentation; Research; Research Personnel; Robot; Robotics; Shapes; Solutions; Sorting - Cell Movement; System; Time; Tissue Engineering; Tissues; Work; cell assembly; cell behavior; cell type; drug discovery; drug testing; flexibility; implantation; improved; in vitro Model; in vivo; magnetic field; micromanipulator; millimeter; novel; research study; scaffold; sonoporation; tissue/cell culture; tool

DESCRIPTION (provided by applicant): The research objective of this proposal is to develop and use optically controlled microbubbles as a microrobotic system that will be used for the microassembly of artificial tissues. The optical control platform developed here will be able to control at least 50 independent microrobots in parallel, which is ten times that of current microrobotic systems. The microrobots will be able to cooperate to accomplish micromanipulation and micro-assembly tasks that can be applied to the manipulation and micro-assembly of biological cells. The micro-assembled cells can be used to create cell and tissue cultures that can be used for artificial tissue growth, drug discovery, and drug testing. The proposed project will focus on the following specific aims: 1) parallel, independent actuation of at least 50 bubble microrobots using automated optical control; 2) microassembly of cell-laden hydrogels using the bubble microrobots; 3) Cell patterning, sorting, and sonoporation using the bubble microrobots; 4) Microassembly of cells using the microrobots to form artificial tissues. The proposed cellular microassembly for tissue engineering will address an obstacle for the development of drug therapies: cells used for drug testing are not fully representative of in vivo cell behavior. The microrobots proposed here will enable the precise yet flexible manipulation and micro-assembly of cells for building artificial tissues, creating more realistic in vitro model, and streamlining the process of developing new drug therapies. The proposed project aligns well with the mission of the National Institute of Biomedical Imaging and Bioengineering (NIBIB). This project integrates engineering and life sciences to create microrobotic systems that can advance basic medical research and medical care. The microrobotic systems will benefit medical researchers by providing them with new tools, enabling previously unrealizable protocols and experiments. The creation of artificial tissues can improve drug discovery and testing, leading to higher-quality medical care.

描述（由申请人提供）：本提案的研究目标是开发和使用光控微泡作为用于人工组织微组装的微型机器人系统。这里开发的光学控制平台将能够并行控制至少50个独立的微型机器人，这是目前微型机器人系统的十倍。微机器人将能够合作完成微操作和微组装任务，可应用于生物细胞的操作和微组装。微组装的细胞可用于创建细胞和组织培养物，其可用于人工组织生长、药物发现和药物测试。拟议的项目将集中在以下具体目标：1）使用自动化光学控制平行，独立驱动至少50个气泡微机器人; 2）使用气泡微机器人微组装载有细胞的水凝胶; 3）使用气泡微机器人进行细胞图案化，分选和声致孔; 4）使用微机器人微组装细胞以形成人工组织。拟议的组织工程细胞微组装将解决药物治疗发展的障碍：用于药物测试的细胞不能完全代表体内细胞行为。本文提出的微型机器人将能够精确而灵活地操纵和微组装细胞，用于构建人工组织，创建更逼真的体外模型，并简化开发新药物疗法的过程。拟议的项目符合国家生物医学成像和生物工程研究所（NIBIB）的使命。该项目将工程和生命科学相结合，创造出能够推进基础医学研究和医疗保健的微型机器人系统。微型机器人系统将为医学研究人员提供新的工具，使他们能够实现以前无法实现的协议和实验。人造组织的创造可以改善药物发现和测试，从而带来更高质量的医疗保健。