Establishing a Role for Kinesin-8 in Mammalian Germ Line Development

描述（由申请人提供）：这个小型研究项目的重点是驱动蛋白8的作用，Kif 18 A，它在细胞分裂中具有重要功能，广泛表达，但在发育中的生殖系中是独特的。哺乳动物Kif 18 A的突变导致生殖细胞耗尽并最终导致不孕;然而，突变小鼠的不孕症并不完全穿透，并且取决于菌株背景。因此，在Kif 18 A消融的背景下，存在改善不孕症的遗传因素。Kif 18 a在生殖系发育中的独特作用最近才被揭示，Kif 18 a在生殖系中的功能机制尚未建立。这个小型研究项目的总体目标是提供建立Kif 18 A在生殖系发育中发挥作用的机制所需的基本生物学数据，并创建一个遗传数据集，用于发现调节Kif 18 A依赖性生殖细胞耗竭的分子途径。这些数据将有助于一个更大的研究计划的基础，专注于生殖细胞分裂的哺乳动物生殖系，其中基因组完整性的维护是不可或缺的配子发生，关键是，生育力的专业化。

DESCRIPTION (provided by applicant): This small research project focuses on the role of kinesin 8, Kif18A, which has an essential function in cell division and is broadly expressed, yet is uniquely required in the developing germ line. Mutations in mammalian Kif18A lead to germ cell depletion and ultimately infertility; however, infertility in mutant mice is not fully penetrat and is dependent on strain background. Therefore, there are genetic factors that ameliorate infertility in the context of Kif18A ablation. The unique role of Kif18a in germ line development has only recently been revealed and a mechanism for Kif18a function in the germ line has not been established. The overall goal of this small research project is to provide the basic biological data required to establish the mechanism by which Kif18A functions in germ line development and to create a genetic dataset for the discovery of the molecular pathways that modulate Kif18A dependent germ cell depletion. These data will contribute to the foundation of a larger research program focused on the specialization of germ cell division in the mammalian germ line, where maintenance of genome integrity is integral to gametogenesis and, critically, fertility.