Visualizing the cardiac conduction network using MR microscopy and diffusion

使用 MR 显微镜和扩散可视化心脏传导网络

基本信息

批准号：
8903567
负责人：
JOHN Robert FORDER
金额：
$ 66.21万
依托单位：
UNIVERSITY OF FLORIDA
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-09-01 至 2016-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8903567
关键词：
Age Anatomy Anisotropy Apex of the Heart Arrhythmia Atlases Brain Cardiac Cardiac Myocytes Cardiac ablation Cardiomyopathies Cells Clinical Clinical Management Congenital Heart Defects Contracts Contrast Media Coupled Data Data Set Detection Diabetes Mellitus Diffusion Female Fiber Florida Future Gender Health Heart Heart Arrest Heart Atrium Heart Diseases Histology Hour Image In Vitro Incidence Internet Intervention Investigation Ischemia Laboratories Lead Left Length Life Location Magnetic Resonance Magnetic Resonance Imaging Maps Methods Microscopy Modeling Monitor Muscle Cells Muscle Contraction Myocardial Contraction Myocardial tissue Myocardium Nature Noise Nutrient Oryctolagus cuniculus Publications Purkinje Cells Relaxation Resolution Running Sampling Series Sex Characteristics Signal Transduction Staining method Stains Structure Structure of purkinje fibers System Techniques Temperature Testing Time Tissue Viability Tissues Validation Ventricular Ventricular septum Work base clinically significant data acquisition design improved in vivo light microscopy male mathematical model neurofilament oxygen transport research study sudden cardiac death tissue fixing young adult

项目摘要

DESCRIPTION (provided by applicant): The heart is responsible for transporting oxygen and nutrients to all tissues in the body, and it performs this function through the coordinated contraction of muscle cells. When coordination of these contractions occurs, the heart fibrillates, or stops beating. The systems of specialized cells that are responsible for this coordination are the cardiac conducting network, commonly referred to as the Purkinje network. These specialized muscle cells rapidly conduct signals to contract, so that the heart tissue at the base of the heart contracts at nearly the same time as tissue at the apex of the heart. Although we have known about these specialized cells for over a hundred years, the only way we are currently able to see them is if we remove the heart, and examine pieces of it under light microscopy. These current techniques are labor intensive, and result in destruction of tissue. We have developed a non-invasive means to determine the anatomical location of these specialized cells using magnetic resonance microscopy and diffusion imaging. Using magnets at the National High Field Magnet Laboratory in Gainesville, Florida, we have obtained the highest resolution MR images to date of myocardial tissue (7um isotropic resolution), and performed diffusion imaging at resolutions down to 40um. We are able to visualize the atrial-ventricular node, conducting bundle, left and right bundle branches, and subsequent fibers that extend down the ventricular septum of the heart and into the ventricular cavities (as free-running Purkinje fibers). We propose to further improve our ability to discriminate these fibers from cardiac muscle cells, and to construct atlases of the conduction network to aid in future modeling. We will also compare the anatomy of this network in both male and female young adults, since the incidence of sudden cardiac death is dramatically different between the genders at this age.

描述（由申请人提供）：心脏负责将氧气和养分运输到体内的所有组织，并且通过肌肉细胞的协调收缩来执行此功能。当发生这些收缩的协调时，心脏颤动，或停止跳动。负责这种协调的专门细胞的系统是心脏传导网络，通常称为Purkinje网络。这些专门的肌肉细胞迅速进行信号以收缩，以使心脏底部的心脏组织与心脏顶点的组织几乎同时收缩。尽管我们已经知道了这些专门的细胞已有一百多年了，但目前能够看到它们的唯一途径是消除心脏，并在光学显微镜下检查其中的部分。这些当前的技术是劳动密集型的，并导致组织破坏。我们已经开发了一种非侵入性手段，可以使用磁共振显微镜和扩散成像来确定这些专业细胞的解剖位置。在佛罗里达州盖恩斯维尔的国家高场磁铁实验室使用磁铁，我们获得了迄今为止心肌组织（7UM各向同性分辨率）的最高分辨率MR图像，并在降至40UM的分辨率下进行了扩散成像。我们能够可视化心房的室里节点，导电束，左右束分支，以及随后延伸到心脏心室隔膜并进入心室腔（作为自由运行的Purkinje纤维）的纤维。我们建议进一步提高我们将这些纤维与心脏肌肉细胞区分开的能力，并构建传导网络的地图，以帮助将来建模。我们还将比较男性和女性年轻人中该网络的解剖结构，因为在这个年龄段的性别之间，心脏猝死的发生率截然不同。